宽频电磁脉冲辐照引起大鼠生殖系统的损伤与修复
Effect of wide-band electromagnetic pulses on the reproductive system of rat and subsequent recovery
- 辐射研究与辐射工艺学报 2017年35卷第1期 页码：10203
- 作者机构：
1.华东师范大学物理与材料科学学院生物物理研究所上海 200241
- 作者简介：
[ "刘合, 男, 1992年9月出生, 2014年6月毕业于苏州大学, 现为华东师范大学在读硕士研究生, 生物物理专业, E-mail:kerwinlh@gmail.com", "LIU He (male) was born in September 1992, and graduated from Soochow University in June 2014. Now he is a mastercandidate in East China Normal University, majoring in Biolophysics, E-mail: kerwinlh@gmail.com" ]
王向晖, 博士, 副教授, E-mail:xhwang@phy.ecnu.edu.cnPh. D. WANG Xianghui, E-mail:xhwang@phy.ecnu.edu.cn
- 基金信息：国家重点基础研究基金项目(2011CB503701);国家自然科学基金青年项目(31600675)
DOI：10.11889/j.1000-3436.2017.rrj.35.010203
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刘合, 雷鸣嘉, 张杰, 等. 宽频电磁脉冲辐照引起大鼠生殖系统的损伤与修复[J]. 辐射研究与辐射工艺学报, 2017,35(1):10203.
He LIU, Mingjia LEI, Jie ZHANG, et al. Effect of wide-band electromagnetic pulses on the reproductive system of rat and subsequent recovery[J]. Journal of Radiation Research and Radiation Processing, 2017,35(1):10203.
刘合, 雷鸣嘉, 张杰, 等. 宽频电磁脉冲辐照引起大鼠生殖系统的损伤与修复[J]. 辐射研究与辐射工艺学报, 2017,35(1):10203. DOI： 10.11889/j.1000-3436.2017.rrj.35.010203.
He LIU, Mingjia LEI, Jie ZHANG, et al. Effect of wide-band electromagnetic pulses on the reproductive system of rat and subsequent recovery[J]. Journal of Radiation Research and Radiation Processing, 2017,35(1):10203. DOI： 10.11889/j.1000-3436.2017.rrj.35.010203.
摘要
采用酶联免疫吸附法测定血清睾酮、精子顶体酶、睾丸组织中超氧化物歧化酶和丙二醛含量；CASA系统检测精子的活力指标；TUNEL法检测睾丸组织中生精细胞的凋亡情况。结果表明，大鼠生殖系统所受到的即时损伤与修复与辐照脉冲的场强和脉冲数有关，场强越高，脉冲数越大，损伤越明显，修复越困难。结果提示，在实验条件下，场强的影响比脉冲次数的影响更显著。
Abstract
The testosterone, superoxide dismutase (SOD), malondialdehyde (MDA), and acrosin activity were measured by ELISA. The cell apoptosis of testicular was observed by TUNEL. The sperm motility was analyzed by CASA. The results showed that the harms caused by W-EMP exposure, and the subsequent recovery were dependent on the intensity and pulse number of W-EMP. With the increase of electric intensity and pulse number the impact of W-EMP exposure increased, which leaded to a slower recovery rate. The results indicates that compared with the pulse number, the electric intensity in present work has much stronger impact on the reproductive system.
关键词
宽频谱电磁脉冲生殖系统精子活力血清睾酮顶体酶
Keywords
Wide-band electromagnetic pulseReproductive systemSperm motilitySerum testosteroneAcrosin
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