重离子束辐照选育高产植物乳酸菌

王雨辰; 王曙阳; 董妙音; 许富强; 胡伟; 陈积红; 李文建; 韩舜愈; 刘敬

doi:10.11889/j.1000-3436.2017.rrj.35.010401

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重离子束辐照选育高产植物乳酸菌

辐射技术的应用 | 更新时间：2021-12-10

- 重离子束辐照选育高产植物乳酸菌
- Study on screening and mutation breeding of high-yield Lactobacillus plantarum by heavy ion irradiation
- 辐射研究与辐射工艺学报 2017年35卷第1期页码：10401
- 作者机构：
  
  1.甘肃农业大学食品科学与工程学院兰州 730070
  2.中国科学院近代物理研究所生物物理室兰州 730000
  3.甘肃省辐照诱变育种工程实验室兰州 730000
- 作者简介：
  
  [ "王雨辰, 女, 1994年10月出生, 2014年6月毕业于上海大学, 现为甘肃农业大学在读硕士研究生, 在中国科学院近代物理研究所做联合培养项目, 营养与食品卫生学专业, 主要从事重离子辐照诱变育种基础研究, E-mail:wangyuchener@impcas.ac.cn", "WANG Yuchen (female) was born in October 1994, and graduated from Shanghai University in June 2014. Now she is amaster candidate in Gansu Agriculture University, combined training of the postgraduate by Institute of Modern Physics, ChineseAcademy of Sciences, majoring in nutrition and food hygiene and focusing on basic study on mutation breeding. E-mail:wangyuchener@impcas.ac.cn" ]
  王曙阳, 博士, 副研究员, E-mail:wangsy@impcas.ac.cnPh.D. WANG Shuyang, associate professor, E-mail:wangsy@impcas.ac.cn
- 基金信息：
  
  国家自然科学基金(11305225);中国科学院科技服务网络计划项目(KFJ-EW-STS-086);西藏草业专项(Y106460XZO)
- DOI：10.11889/j.1000-3436.2017.rrj.35.010401
  中图分类号：
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王雨辰, 王曙阳, 董妙音, 等. 重离子束辐照选育高产植物乳酸菌[J]. 辐射研究与辐射工艺学报, 2017,35(1):10401.

Yuchen WANG, Shuyang WANG, Miaoyin DONG, et al. Study on screening and mutation breeding of high-yield Lactobacillus plantarum by heavy ion irradiation[J]. Journal of Radiation Research and Radiation Processing, 2017,35(1):10401.
王雨辰, 王曙阳, 董妙音, 等. 重离子束辐照选育高产植物乳酸菌[J]. 辐射研究与辐射工艺学报, 2017,35(1):10401. DOI： 10.11889/j.1000-3436.2017.rrj.35.010401.

Yuchen WANG, Shuyang WANG, Miaoyin DONG, et al. Study on screening and mutation breeding of high-yield Lactobacillus plantarum by heavy ion irradiation[J]. Journal of Radiation Research and Radiation Processing, 2017,35(1):10401. DOI： 10.11889/j.1000-3436.2017.rrj.35.010401.

摘要

采用重离子束,12,C,6+,对乳酸菌出发菌株JTL进行辐照选育。确定吸收剂量300 Gy时最优，在该吸收剂量下，出发菌种的正突变率和致死率均达到最高，分别为34.4%、80%。通过酸斑法初筛和发酵复筛法，选育出高产酸的3株突变菌株（JTL2、JTL3、JTL5），其发酵液中乳酸含量比出发菌株JTL提高了41.29%~49.64%。同时，经过传代实验证实，3株菌株具有良好的遗传稳定性。研究表明，利用重离子束,12,C,6+,辐照处理乳酸菌可以得到高产酸的突变菌株，诱变效果显著。

Abstract

12,C,6+, ion beam was used to irradiate strains of ,Lactobacillus plantarum, JTL, and the results showed that the strain could get the highest positive mutation rate and lethality with 34.4% and 80% at the optimal absorbed dose of 300 Gy. The strains which could produce higher lactic acid were screened by selective MRS mediums and fermentation experiments. Finally, three mutations JTL2, JTL3 and JTL5 were obtained through the two screening steps of selecting plate and fermentation, and the contents of lactic acid in this three fermentation broth were greater than the original strain by 41.29% to 49.64%. The pass-generation test indicated that the heredity character of these mutations were stable. It is demonstrated that the lactic acids produced by ,Lactobacillus plantarum, JTL were remarkably improved, and heavy ion beam is an efficient radiation resource for microorganism breeding.

关键词

乳酸菌重离子束辐照诱变育种

Keywords

Lactobacillus plantarumHeavy ion beamIrradiationMutation breeding

references

金世琳 . 乳酸菌的科学与技术 . 中国乳制品工业 , 1998 . 26 (2 ):14 -16 . http://www.cnki.com.cn/Article/CJFDTOTAL-RPGY802.004.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-RPGY802.004.htm, .

Shilin JIN . Science and technology of lactic acid bacteria . China Diary Industry , 1998 . 26 (2 ):14 -16 . http://www.cnki.com.cn/Article/CJFDTOTAL-RPGY802.004.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-RPGY802.004.htm, .

X J Li , L Y Yue , X F Guan , 等 . The adhesion of putative probiotic lactobacillus to cultured epithelial cells and porcine intestinal mucus . Journal of Applied Microbiology , 2008 . 104 (4 ):1082 -1091 . DOI:10.1111/j.1365-2672.2007.03636.xhttp://doi.org/10.1111/j.1365-2672.2007.03636.x.

G J O Jansen . Mutator activity in uvs mutants of Aspergillus nidulans . Molecular and General Genetics , 1972 . 116 (1 ):47 -50 . DOI:10.1007/BF00334259http://doi.org/10.1007/BF00334259.

孙洁 , 吕加平 , 刘鹭 , 等 . N+注入诱变高自溶度的乳酸菌突变株 . 核农学报 , 2010 . 24 (4 ):684 -688. .

Jie SUN , Jiaping LYU , Lu LIU , 等 . Screening of Lactic acid bacteria with high autolysis rate by N+-implantation . Journal of Nuclear Agricultural Science , 2010 . 24 (4 ):684 -688. .

刘月英 , 赵士豪 , 关中波 . 泡菜乳酸菌株的诱变选育 . 食品科学 , 2008 . 29 (9 ):431 -433 . http://www.cnki.com.cn/Article/CJFDTOTAL-SPKX200809099.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-SPKX200809099.htm, .

Yueying LIU , Shihao ZHAO , Zhongbo GUAN . Mutation screening of lactobacillus for production of radish pickle . Food Science , 2008 . 29 (9 ):431 -433 . http://www.cnki.com.cn/Article/CJFDTOTAL-SPKX200809099.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-SPKX200809099.htm, .

宋云 , 张怀渝 , 畅志坚 . 离子束用于诱变育种的研究进展 . 分子植物育种 , 2004 . 2 (2 ):301 -305 . http://www.cnki.com.cn/Article/CJFDTOTAL-FZZW200402034.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-FZZW200402034.htm, .

Yun SONG , Huaiyu ZHANG , Zhijian CHANG . Progress of mutation breeding with ion beam implantation . Molecular Plant Breeding , 2004 . 2 (2 ):301 -305 . http://www.cnki.com.cn/Article/CJFDTOTAL-FZZW200402034.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-FZZW200402034.htm, .

Yiguang CHEN , Wenjun LI , Lihua XU , 等 . Isolation and phylogenetic analysis of one actinomycete strain YIM 90022 exhibiting anticancer activity . Acta Microbiologica Sinica (in China) , 2006 . 46 (5 ):696 -701 . https://www.researchgate.net/publication/6628071_Isolation_and_phylogenetic_analysis_of_one_actinomycete_strain_YIM_90022_exhibiting_anticancer_activityhttps://www.researchgate.net/publication/6628071_Isolation_and_phylogenetic_analysis_of_one_actinomycete_strain_YIM_90022_exhibiting_anticancer_activity, .

朱传合 , 贺亚男 , 路福平 , 等 . N+离子注入阿维拉霉素产生菌诱变效应的研究 . 核技术 , 2006 . 29 (8 ):609 -613. .

Chuanhe ZHU , Yanan HE , Fuping LU , 等 . Study on mutagenic effects of avilamycin-producing strain induced by N+ ion implantation . Nuclear Techniques , 2006 . 29 (8 ):609 -613. .

袁成凌 , 余增亮 . 低能离子束在生物技术中的应用研究 . 中国生物工程杂志 , 2003 . 23 (4 ):57 -61 . http://www.cnki.com.cn/Article/CJFDTOTAL-SWGJ200304012.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-SWGJ200304012.htm, .

Chengling YUAN , Zengliang YU . The grand progress of low-energy ion beam in life sciences . China Biotechnology , 2003 . 23 (4 ):57 -61 . http://www.cnki.com.cn/Article/CJFDTOTAL-SWGJ200304012.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-SWGJ200304012.htm, .

余增亮 . 离子束生物技术引论 , :合肥安徽科学技术出版社 , 1998 . 67 -105. .

Zengliang YU . Introduction to ion beam biotechnology , :Hefei Anhui Education Press , 1998 . 67 -105. .

Zengliang YU . Introduction to ion beam biotechnology , :New York Springer Press , 2006 . 127 -128. .

蒋彬 , 罗正明 . 辐射治疗能区内电子束在轻介质中的平均能量计算 . 辐射研究与辐射工艺学报 , 1994 . 12 (4 ):232 -238 . http://www.cnki.com.cn/Article/CJFDTOTAL-FYFG404.008.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-FYFG404.008.htm, .

Bin JIANG , Zhengming LUO . The calculation of mean energy for electron beam in the energy range of radio therapy in light media . Journal of Radiation Research and Radiation Processing , 1994 . 12 (4 ):232 -238 . http://www.cnki.com.cn/Article/CJFDTOTAL-FYFG404.008.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-FYFG404.008.htm, .

胡伟 , 陈积红 , 张珍 , 等 . 重离子辐照柠檬酸菌株的诱变选育 . 辐射研究与辐射工艺学报 , 2012 . 30 (1 ):53 -57 . http://www.cnki.com.cn/Article/CJFDTOTAL-FYFG201201011.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-FYFG201201011.htm, .

Wei HU , Jihong CHEN , Zhen ZHANG , 等 . Mutation breeding of Aspergillus niger for producing citric acid by heavy ion beam . Journal of Radiation Research and Radiation Processing , 2012 . 30 (1 ):53 -57 . http://www.cnki.com.cn/Article/CJFDTOTAL-FYFG201201011.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-FYFG201201011.htm, .

樊永红 , 王丽 , 柳丹 , 等 . 米根霉发酵液中乳酸含量的测定方法研究 . 生物技术 , 2007 . 17 (1 ):54 -55 . http://www.cnki.com.cn/Article/CJFDTOTAL-SWJS200701018.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-SWJS200701018.htm, .

Yonghong FAN , Li WANG , Dan LIU , 等 . Studies on analytical methods of lactic acid content in fermentationbroth . Biotechnology , 2007 . 17 (1 ):54 -55 . http://www.cnki.com.cn/Article/CJFDTOTAL-SWJS200701018.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-SWJS200701018.htm, .

吴庆华 , 陈积红 , 张珍 , 等 . X射线对嗜热乳杆菌产L-乳酸的选育研究 . 食品工业科技 , 2015 . 36 (3 ):116 -118 . http://www.cnki.com.cn/Article/CJFDTOTAL-SPKJ201503025.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-SPKJ201503025.htm, .

Qinghua WU , Jihong CHEN , Zhen ZHANG , 等 . Breeding study of Lactobacillus thermophilius induced by X-ray for L-lactic acid production . Science and Technology of Food Industry , 2015 . 36 (3 ):116 -118 . http://www.cnki.com.cn/Article/CJFDTOTAL-SPKJ201503025.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-SPKJ201503025.htm, .

惠友权 , 黄建新 , 孔锁贤 . 离子注入选育α-乙酰乳酸脱羧酶菌株 . 西北大学学报(自然科学版) , 2001 . 31 (3 ):251 -254 . http://www.cnki.com.cn/Article/CJFDTOTAL-XBDZ200103021.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-XBDZ200103021.htm, .

Youquan HUI , Jianxin HUANG , Suoxian KONG . Selective breeding of microbial strain with high yield of α-acetolactate decarboxylase by ion implantation . Journal of Northwest University (Natural Science Edition) , 2001 . 31 (3 ):251 -254 . http://www.cnki.com.cn/Article/CJFDTOTAL-XBDZ200103021.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-XBDZ200103021.htm, .

虞龙 , 许安 . 低能离子注入在Vc高产菌株选育中的应用 . 激光生物学报 , 1999 . 8 (3 ):217 -220 . http://www.cnki.com.cn/Article/CJFDTOTAL-JGSW199903014.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-JGSW199903014.htm, .

Long YU , An XU . The application of low energy ion implantation in breeding of high yield Vc strains . Acta Laser Biology Sinica , 1999 . 8 (3 ):217 -220 . http://www.cnki.com.cn/Article/CJFDTOTAL-JGSW199903014.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-JGSW199903014.htm, .

杜严华 , 丘冠英 , 李国红 , 等 . 低能离子束及γ辐照对小菜蛾颗粒体病毒的影响及剂量效应关系的拟合分析 . 生物物理学报 , 1997 . 13 (2 ):267 -272 . http://www.cnki.com.cn/Article/CJFDTOTAL-SWWL702.018.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-SWWL702.018.htm, .

Yanhua DU , Guanying QIU , Guohong LI , 等 . The fitting analysis of low energy ion implantation and γ-radiation cause the effect of Plutella xylostella Granulosis virus . Acta Biophysica Sinica , 1997 . 13 (2 ):267 -272 . http://www.cnki.com.cn/Article/CJFDTOTAL-SWWL702.018.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-SWWL702.018.htm, .

宋道军 , 姚建铭 , 邵春林 , 等 . 离子注入微生物产生"马鞍型"存活曲线的可能作用机制 . 核技术 , 1999 . 2 (3 ):129 -132 . http://www.cnki.com.cn/Article/CJFDTOTAL-HJSU199903000.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-HJSU199903000.htm, .

Daojun SONG , Jianming YAO , Chunlin SHAO , 等 . A possible mechanism of dose related survival of microorganism implanted by N+ ions . Nuclear Techniques , 1999 . 2 (3 ):129 -132 . http://www.cnki.com.cn/Article/CJFDTOTAL-HJSU199903000.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-HJSU199903000.htm, .

余增亮 . 离子束与生命科学--一个新的研究领域 . 物理 , 1997 . 21 (6 ):241 -246 . http://www.cnki.com.cn/Article/CJFDTOTAL-WLZZ706.003.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-WLZZ706.003.htm, .

Zengliang YU . Ion beam and life science--a new research field . Physics , 1997 . 21 (6 ):241 -246 . http://www.cnki.com.cn/Article/CJFDTOTAL-WLZZ706.003.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-WLZZ706.003.htm, .

Z L YU . Ion beam and life science . Physics (in China) , 1997 . 18 (6 ):333 -338. .

蔡聪 , 姜婷 , 郑兆娟 , 等 . 等离子体诱变凝结芽孢杆菌提高木糖利用能力高产L-乳酸 . 食品科学 , 2014 . 35 (1 ):125 -129 . http://www.cnki.com.cn/Article/CJFDTOTAL-SPKX201401025.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-SPKX201401025.htm, .

Cong CAI , Ting JIANG , Zhaojuan ZHENG , 等 . Improved Xylose utilization of Bacillus coagulans by atmospheric and room temperature plasma mutation for production of Lactic acid . Food Science , 2014 . 35 (1 ):125 -129 . http://www.cnki.com.cn/Article/CJFDTOTAL-SPKX201401025.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-SPKX201401025.htm, .

李元媛 , 高媛 , 蔡俊 . 理化诱变提高鼠李糖乳杆菌产L-乳酸的研究 . 湖北农业科学 , 2010 . 49 (1 ):70 -73 . http://www.cnki.com.cn/Article/CJFDTOTAL-HBNY201001026.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-HBNY201001026.htm, .

Yuanyuan LI , Yuan GAO , Jun CAI . Mutation breeding of high-yield strains of L-lactic acid . Hubei Agricultural Sciences , 2010 . 49 (1 ):70 -73 . http://www.cnki.com.cn/Article/CJFDTOTAL-HBNY201001026.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-HBNY201001026.htm, .

庞锐 , 潘丽军 , 姜绍通 , 等 . N+注入诱变选育混合糖发酵L-乳酸高产菌株 . 食品科学 , 2010 . 31 (21 ):248 .

Rui PANG , Lijun PAN , Shaotong JIANG , 等 . Breeding of high-yield L-lactic acid strains for co-fermentation of glucose and Xylose by N+ implantation . Food Science , 2010 . 31 (21 ):248 .

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