低能氮离子注入和紫外线复合诱变选育高产酿酒酵母

虞龙; 姚驰亚; 吴晓菲; 李玉燕; 张娣; 虞沐尧

doi:10.11889/j.1000-3436.2017.rrj.35.020401

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低能氮离子注入和紫外线复合诱变选育高产酿酒酵母

辐射技术的应用 | 更新时间：2021-12-10

- 低能氮离子注入和紫外线复合诱变选育高产酿酒酵母
- Mutation breeding of ethanol-producing yeast Saccharomyces cerevisiae by low-energy nitrogen ion implantation and UV rays
- 辐射研究与辐射工艺学报 2017年35卷第2期页码：20401
- 作者机构：
  
  1.南京工业大学生物与制药工程学院南京 210000
- 作者简介：
  
  [ "虞龙, 男, 1970年2月出生, 2002年于中科院获得博士学位, 教授, E-mail:lzsswgc@163.com", "YU Long (male) was born in February 1970, and received his doctor's degree from Chinese Academy of Science in 2002, professor, E-mail:lzsswgc@163.com" ]
- 基金信息：
  
  国家高技术研究发展计划（863计划）(2012AA023400)
- DOI：10.11889/j.1000-3436.2017.rrj.35.020401
  中图分类号：
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虞龙, 姚驰亚, 吴晓菲, 等. 低能氮离子注入和紫外线复合诱变选育高产酿酒酵母[J]. 辐射研究与辐射工艺学报, 2017,35(2):20401.

Long YU, Chiya YAO, Xiaofei WU, et al. Mutation breeding of ethanol-producing yeast Saccharomyces cerevisiae by low-energy nitrogen ion implantation and UV rays[J]. Journal of Radiation Research and Radiation Processing, 2017,35(2):20401.
虞龙, 姚驰亚, 吴晓菲, 等. 低能氮离子注入和紫外线复合诱变选育高产酿酒酵母[J]. 辐射研究与辐射工艺学报, 2017,35(2):20401. DOI： 10.11889/j.1000-3436.2017.rrj.35.020401.

Long YU, Chiya YAO, Xiaofei WU, et al. Mutation breeding of ethanol-producing yeast Saccharomyces cerevisiae by low-energy nitrogen ion implantation and UV rays[J]. Journal of Radiation Research and Radiation Processing, 2017,35(2):20401. DOI： 10.11889/j.1000-3436.2017.rrj.35.020401.

摘要

通过离子注入联合紫外线辐射诱变选育高产酿酒酵母菌株，根据存活率及突变率确定离子注入的诱变条件为电荷18 keV，剂量400×10,14, cm,-2,，紫外线辐射60s。在该条件下，诱变获得的菌株正突变率和致死率都最高，诱变效果最好。在该条件下进行研究后获得目标菌株SC-N9015，酒精产量从14.65%提升到15.53%。多次传代实验结果显示遗传性状稳定。研究表明，通过低能氮离子注入和紫外线联合诱变选育高产酒精酵母效果显著。

Abstract

The ethanol-producing yeast ,Saccharomyces cerevisiae, was induced by N,+, implantation with an output power of 18 keV and a dose of 400×10,14, cm,-2, depending on the survival and mutation rates. The UV exposure time was 60 s. Under optimized conditions, the strain achieved the highest positive mutation and lethality rates. A mutant strain SC-N9015 was selected; the ethanol yield of this strain was 15.53%, whereas that of the parental strain was 14.65%. Further, evaluation of ethanol yield over successive generations indicated the hereditary stability of the mutations. The study shows that the combination of low energy N,+, implantation with UV rays is an efficient method for mutation breeding of ,S. cerevisiae,.

关键词

酿酒酵母低能氮离子注入紫外线辐射诱变筛选

Keywords

Saccharomyces cerevisiaeLow energy N+ implantationUV raysIrradiationMutation breeding

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