碳离子束与X射线对女性恶性肿瘤患者外周血淋巴细胞亚群的影响

董方; 高力英; 张红; 王涛; 郭红云; 冉俊涛; 郭浩楠

doi:10.11889/j.1000-3436.2017.rrj.35.030202

您当前的位置：

首页 >

文章列表页 >

碳离子束与X射线对女性恶性肿瘤患者外周血淋巴细胞亚群的影响

辐射生物学与医学 | 更新时间：2021-12-10

- 碳离子束与X射线对女性恶性肿瘤患者外周血淋巴细胞亚群的影响
- Effects of carbon ion beams and X-rays on peripheral blood lymphocyte subsets in female patients with malignant tumors
- 辐射研究与辐射工艺学报 2017年35卷第3期
- 作者机构：
  
  1.甘肃省肿瘤医院放疗科兰州 730050
  2.中国科学院近代物理研究所兰州 730000
  3.甘肃省医学科学研究院兰州 730050
- 作者简介：
  
  [ "董方, 女, 1981年9月出生, 2014年于兰州大学获得肿瘤学硕士学位, 甘肃省肿瘤医院放疗科主治医师, 从事肿瘤放射治疗临床及基础研究, E-mail:dfpaulinekeyan@163.com", "DONG Fang (female) was born in September 1981, and received her master's degree in oncology from Lanzhou University in 2014. Now she works at the Department of Radiation Oncology of Gansu Provincial Cancer Hospital as an attending radiotherapy doctor, engaging in the field of clinical and basic research of radiation oncology. E-mail: dfpaulinekeyan@163.com" ]
  高力英, 主任医师, E-mail:lygaolanzhou@163.com GAO Liying, chief physician, E-mail: lygaolanzhou@163.com
- 基金信息：
  
  甘肃省技术研究与开发专项计划项目(1305TCYA009)
- DOI：10.11889/j.1000-3436.2017.rrj.35.030202
  中图分类号：
扫描看全文
董方, 高力英, 张红, 等. 碳离子束与X射线对女性恶性肿瘤患者外周血淋巴细胞亚群的影响[J]. 辐射研究与辐射工艺学报, 2017,35(3):030202-7.

Fang DONG, Liying GAO, Hong ZHANG, et al. Effects of carbon ion beams and X-rays on peripheral blood lymphocyte subsets in female patients with malignant tumors[J]. Journal of Radiation Research and Radiation Processing, 2017,35(3):030202-7.
董方, 高力英, 张红, 等. 碳离子束与X射线对女性恶性肿瘤患者外周血淋巴细胞亚群的影响[J]. 辐射研究与辐射工艺学报, 2017,35(3):030202-7. DOI： 10.11889/j.1000-3436.2017.rrj.35.030202.

Fang DONG, Liying GAO, Hong ZHANG, et al. Effects of carbon ion beams and X-rays on peripheral blood lymphocyte subsets in female patients with malignant tumors[J]. Journal of Radiation Research and Radiation Processing, 2017,35(3):030202-7. DOI： 10.11889/j.1000-3436.2017.rrj.35.030202.

摘要

分析女性乳腺癌及宫颈癌患者外周血在接受不同剂量碳离子束与X射线辐射前后淋巴细胞亚群数值的变化。抽取5例宫颈癌、5例女性乳腺癌患者的外周静脉血，经照射后（设未接受辐射组、1、2 GyE碳离子束组、及1、2 Gy的X射线组）进行淋巴细胞亚群检测。采用独立样本,t,检验，分别对两组离体血样本进行CD,3+,、CD,8+,、CD,4+,、NK细胞、B细胞、CD,4+,/CD,8+,两两组间对比，分析数值变化情况。结果显示：两组离体血在接受不同射线、不同剂量照射后，淋巴细胞亚群检测结果差异无显著性的意义（,p,>,0.05）；接受高线性传能密度（Linear energy transfer，LET）碳离子照射时，乳腺癌组中CD,4+,值随剂量增加而升高，宫颈癌组数值则表现出相反的趋势；接受6 MV-X射线照射时，宫颈癌组中NK细胞数值随剂量增加而下降，而乳腺癌组则相反；接受碳离子束照射时，两病种受到1 GyE照射后NK细胞数值均较未照射组数值升高，2 GyE则均较未照射组数值下降。结果提示：女性生殖系统最常见两种恶性肿瘤离体外周血对高LET碳离子和低LET的6 MV-X射线照射表现出了不同的免疫应答反应。

Abstract

Peripheral blood that had been exposed to different doses of carbon ion beams and X-rays in female patients with breast cancer and cervical cancer was analyzed to observe the changes in lymphocyte subsets. The peripheral venous blood from women with cervical cancer (5 patients) and breast cancer (5 patients) was irradiated with carbon ion beams at 1, 2 GyE, X-rays at 1, 2 Gy, and 0 Gy (control), respectively, to test the lymphocyte subgroups. Using independent sample t-test to analyze the changes of CD,3+, CD,8+, CD,4+, NK cells, B cells, and CD,4+,/CD,8+, in the two ,in-vitro, blood samples, respectively. The results showed that there were no significant differences (,p,>,0.05) in the values in ,in-vitro, blood of lymphocyte subgroup after exposure to carbon ion beams and X-rays at different doses. The CD,4+, value increased with the dose in the breast cancer group and decreased in the cervical cancer group when irradiated with high linear-energy-transfer (LET) carbon ion beams. The NK cell value increased with the dose in the breast cancer group and decreased in the cervical cancer group when irradiated with 6 MV X-rays. The NK cell value was higher in 1 GyE dose group than that in the control group, while lower in 2 GyE dose group when irradiated with carbon ion beams. These results indicate that the ,in-vitro, peripheral blood of the most common two malignant tumors in female reproductive system irradiated with high LET carbon ions and low LET 6 MV X-rays shows different immune response.

关键词

女性恶性肿瘤淋巴细胞亚群放射治疗碳离子束X射线

Keywords

FemaleMalignant tumorLymphocyte subsetsRadiotherapyCarbon ion beamX-rays

references

T Sugane , M Baba , R Imai , 等 . Carbon ion radiotherapy for elderly patients 80 years and older with stage Ⅰ non-small cell lung cancer . Lung Cancer , 2009 . 64 (1 ):45 -50 . DOI:10.1016/j.lungcan.2008.07.007http://doi.org/10.1016/j.lungcan.2008.07.007 .

T Yanagi , J E Mizoe , A Hasegawa , 等 . Mucosal malignant melanoma of the head and neck treated by carbon ion radiotherapy . International Journal of Radiation Oncology Biology Physics , 2009 . 74 (1 ):15 -20 . DOI:10.1016/j.ijrobp.2008.07.056http://doi.org/10.1016/j.ijrobp.2008.07.056 .

K Jingu , A Hasegawa , J E Mizo , 等 . Carbon ion radiotherapy for basal cell adenocarcinoma of the head and neck . Radiation Oncology , 2010 . 5 (1 ):89 DOI:10.1186/1748-717X-5-89http://doi.org/10.1186/1748-717X-5-89 .

M W Münter , M Wengenroth , G Fehrenbacher , 等 . Heavy ion radiotherapy during pregnancy . Fertility and Sterility , 2010 . 94 (6 ):2329 DOI:10.1016/j.fertnstert.2010.04.005http://doi.org/10.1016/j.fertnstert.2010.04.005 .

B Wang , K Tanaka , G Varès , 等 . X-ray-induced radioresistance against high-LET radiations from accelerated heavy ions in mice . Radiation Research , 2010 . 174 (4 ):532 -536 . DOI:10.1667/RR2133.1http://doi.org/10.1667/RR2133.1 .

N Hamada , T Hara , M Omura-Minamisawa , 等 . Energetic heavy ions overcome tumor radio resistance caused by over expression of Bcl-2 . Radiotherapy and Oncology , 2008 . 89 (2 ):231 -236 . DOI:10.1016/j.radonc.2008.02.013http://doi.org/10.1016/j.radonc.2008.02.013 .

乐紫妤 , 刘勇 . 重离子肿瘤放射生物学的研究进展 . 中华放射肿瘤学杂志 , 2015 . 24 (5 ):600 -604 . DOI:10.3760/cma.j.issn.1004-4221.2015.05.032http://doi.org/10.3760/cma.j.issn.1004-4221.2015.05.032 .

Ziyu YUE , Yong LIU . The research progress of tumor radiobiology of heavy ions . Chinese Journal of Radiation Oncology , 2015 . 24 (5 ):600 -604 . DOI:10.3760/cma.j.issn.1004-4221.2015.05.032http://doi.org/10.3760/cma.j.issn.1004-4221.2015.05.032 .

C X Di , L N Yang , H Zhang , 等 . Effects of carbon-ion beam or X-ray irradiation on anti-apoptosis DeltaNp73 expression in HeLa cell . Gene , 2013 . 515 (1 ):208 -213 . DOI:10.1016/j.gene.2012.11.040http://doi.org/10.1016/j.gene.2012.11.040 .

D Schulz-Ertner , H Tsujii . Particle radiation therapy using proton and heavier ion beams . Journal of Clinical Oncology , 2007 . 25 (8 ):953 -964 . DOI:10.1200/JCO.2006.09.7816http://doi.org/10.1200/JCO.2006.09.7816 .

D S Dizon , L Krilov , E Cohen , 等 . Clinical cancer advances 2016:annual report on progress against cancer from the American Society of Clinical Oncology . Journal of Clinical Oncology , 2016 . 34 (9 ):987 -1011 . DOI:10.1200/JCO.2015.65.8427http://doi.org/10.1200/JCO.2015.65.8427 .

M A Postow , M K Callahan , C A Barker , 等 . Immu-nologic correlates of the abscopal effect in a patient with melanoma . The New England Journal of Medicine , 2012 . 366 (10 ):925 -931 . DOI:10.1056/NEJMoa1112824http://doi.org/10.1056/NEJMoa1112824 .

冯佳 , 彭敏 , 宋启斌 . 放射治疗联合免疫疗法治疗恶性肿瘤的相关机制与研究进展 . 现代肿瘤医学 , 2017 . 25 (3 ):474 -478 . DOI:10.3969/j.issn.1672-4992.2017.03.037http://doi.org/10.3969/j.issn.1672-4992.2017.03.037 .

Jia FENG , Min PENG , Qibin SONG . Mechanisms and progress of radiotherapy plus immunotherapy treating malignant tumors . Journal of Modern Oncology , 2017 . 25 (3 ):474 -478 . DOI:10.3969/j.issn.1672-4992.2017.03.037http://doi.org/10.3969/j.issn.1672-4992.2017.03.037 .

李益民 , 高力英 , 王小虎 , 等 . 碳离子束放射治疗对恶性肿瘤患者T细胞亚群的影响 . 现代肿瘤医学 , 2013 . 21 (5 ):1115 -1117 . DOI:10.7644/j.issn.1674-9960.2014.08.015http://doi.org/10.7644/j.issn.1674-9960.2014.08.015 .

Yimin LI , Liying GAO , Xiaohu WANG , 等 . Influence of carbon ion beam radiation therapy on T-cell subsets in patients with malignant tumor . Journal of Modern Oncology , 2013 . 21 (5 ):1115 -1117 . DOI:10.7644/j.issn.1674-9960.2014.08.015http://doi.org/10.7644/j.issn.1674-9960.2014.08.015 .

M A Postow , M K Callahan , C A Barker , 等 . Immunologic correlates of the abscopal effect in a patient with melanoma . The New England Journal of Medicine , 2012 . 366 (10 ):925 -931 . DOI:10.1056/NEJMoa1112824http://doi.org/10.1056/NEJMoa1112824 .

肖凤君 , 杨月峰 , 王华 , 等 . 重离子辐射对人外周血T淋巴细胞生物学性能的影响 . 军事医学 , 2014 . 38 (8 ):630 -637 . DOI:10.7644/j.issn.1674-9960.2014.08.015http://doi.org/10.7644/j.issn.1674-9960.2014.08.015 .

Fengjun XIAO , Yuefeng YANG , Hua WANG , 等 . Biologic effect of heavy ion radiation on human peripheral blood derived T lymphocytes . Military Medical Sciences , 2014 . 38 (8 ):630 -637. .

彭效祥 , 穆向魁 , 赵美红 , 等 . 质子放疗前后恶性肿瘤患者T细胞亚群的变化 . 医学检验与临床 , 2008 . 19 (2 ):16 -18 . DOI:10.3969/j.issn.1673-5013.2008.02.005http://doi.org/10.3969/j.issn.1673-5013.2008.02.005 .

Xiaoxiang PENG , Xiangkui MU , Meihong ZHAO , 等 . T-cell subgroups of the malignant tumor patients changes before and after proton radiotherapy . Medical Laboratory Science and Clinics , 2008 . 19 (2 ):16 -18 . DOI:10.3969/j.issn.1673-5013.2008.02.005http://doi.org/10.3969/j.issn.1673-5013.2008.02.005 .

邴涛 , 党秉荣 , 谢漪 , 等 . 80 MeV/u 12C6+离子辐照小鼠头部对脾脏细胞周期分布的影响 . 辐射研究与辐射工艺学报 , 2006 . 24 (1 ):61 -63 . DOI:10.3969/j.issn.1000-3436.2006.01.014http://doi.org/10.3969/j.issn.1000-3436.2006.01.014 .

Tao BING , Bingrong DANG , Yi XIE , 等 . Spleen cell cycle changes of mice irradiated at head by 80 MeV/u 12C6+ ions . Journal of Radiation Research and Radiation Pocessing , 2006 . 24 (1 ):61 -63 . DOI:10.3969/j.issn.1000-3436.2006.01.014http://doi.org/10.3969/j.issn.1000-3436.2006.01.014 .

赵卫平 , 张红 , 王燕玲 , 等 . 低剂量12C6+离子辐照对小鼠胸腺、脾脏细胞周期及DNA损伤的影响 . 辐射研究与辐射工艺学报 , 2008 . 26 (6 ):343 -347 . DOI:10.3969/j.issn.1000-3436.2008.06.005http://doi.org/10.3969/j.issn.1000-3436.2008.06.005 .

Weiping ZHAO , Hong ZHANG , Yanling WANG , 等 . Effect of low dose 12C6+ irradiation on cell cycle and DNA damage in mouse thymus and spleen cells . Journal of Radiation Research and Radiation Pocessing , 2008 . 26 (6 ):343 -347 . DOI:10.3969/j.issn.1000-3436.2008.06.005http://doi.org/10.3969/j.issn.1000-3436.2008.06.005 .

高力英 , 冉俊涛 , 郭浩男 , 等 . 碳离子束和X射线对头颈癌患者与健康人离体外周血淋巴细胞亚群影响的对比研究 . 现代肿瘤医学 , 2016 . 24 (20 ):3188 -3192 . DOI:10.3969/j.issn.1672-4992.2016.20.005http://doi.org/10.3969/j.issn.1672-4992.2016.20.005 .

Liying GAO , Juntao RAN , Haonan GUO , 等 . Vitro comparative study of carbon ion beam and X-ray for head and neck cancer and healthy about the impact of peripheral blood lymphocyte subsets . Journal of Modern Oncology , 2016 . 24 (20 ):3188 -3192 . DOI:10.3969/j.issn.1672-4992.2016.20.005http://doi.org/10.3969/j.issn.1672-4992.2016.20.005 .

张晓文 , 郭红云 , 王小琦 , 等 . 低剂量12C6+离子辐射诱导的小鼠骨髓细胞适应 . 临医学工程 , 2011 . 4 (18 ):481 -483 . DOI:10.3969/j.issn.1674-4659.2011.04.0481http://doi.org/10.3969/j.issn.1674-4659.2011.04.0481 .

Xiaowen ZHANG , Hongyun GUO , Xiaoqi WANG , 等 . Experimental study on adaptive response of bone marrow cells induced by low-dose 12C6+ ion beam radiation in mice . Clinical Medical Engineering , 2011 . 4 (18 ):481 -483 . DOI:10.3969/j.issn.1674-4659.2011.04.0481http://doi.org/10.3969/j.issn.1674-4659.2011.04.0481 .

H Zhang , B Liu , Q Zhou , 等 . Alleviation of preexposure of mouse brain with low-dose 12C6+ or 60Co gamma-ray on male reproductive endocrine damages induced by subsequent high dose irradiation . International Journal of Andrology , 2006 . 29 (6 ):592 -596 . DOI:10.1111/j.1365-2605.2006.00698.xhttp://doi.org/10.1111/j.1365-2605.2006.00698.x .

李晓华 , 蒙以良 , 黄承乐 . 肿瘤放疗患者外周血T细胞亚群研究进展 . 右江民族医学院学报 , 2012 . 34 (4 ):526 -528 . DOI:10.3969/j.issn.1001-5817.2012.04.050http://doi.org/10.3969/j.issn.1001-5817.2012.04.050 .

Xiaohua LI , Yiliang MENG , Chengle HUANG . The research progress on the tumor radiotherapy in patients with peripheral blood T cell subsets . Journal of You-jiang Medical University for Nationalities , 2012 . 34 (4 ):526 -528 . DOI:10.3969/j.issn.1001-5817.2012.04.050http://doi.org/10.3969/j.issn.1001-5817.2012.04.050 .

林飞卿 , 余传霖 , 何球藻 . 医学基础免疫学 , :上海上海医科大学出版杜 , 1992 . 72 -79. .

Feiqing LIN , Chuanlin YU , Qiuzao HE . Basic medical immunology , :Shanghai Shanghai Medical University Press , 1992 . 72 -79. .

刘小林 , 王忠明 . 宫颈癌患者淋巴细胞亚群检测的临床意义 . 放射免疫学杂志 , 2010 . 23 (5 ):592 -593 . DOI:10.3969/j.issn.1008-9810.2010.05.066http://doi.org/10.3969/j.issn.1008-9810.2010.05.066 .

Xiaolin LIU , Zhongming WANG . Clinical significance for lymphocyte subsets of patients with cervical cancer detection . Journal of Radioimmunology , 2010 . 23 (5 ):592 -593 . DOI:10.3969/j.issn.1008-9810.2010.05.066http://doi.org/10.3969/j.issn.1008-9810.2010.05.066 .

孙林 , 温江涛 , 刘海红 , 等 . 乳腺癌患者外周血T淋巴细胞及NK细胞的检测及其临床意义 . 现代肿瘤医学 , 2006 . 14 (9 ):1069 -1071 . DOI:10.3969/j.issn.1672-4992.2006.09.009http://doi.org/10.3969/j.issn.1672-4992.2006.09.009 .

Lin SUN , Jiangtao WEN , Haihong LIU . Detection of T lymphocyte subset and NK cell in peripheral blood of the patients with breast cancer . Journal of Modern Oncology , 2006 . 14 (9 ):1069 -1071 . DOI:10.3969/j.issn.1672-4992.2006.09.009http://doi.org/10.3969/j.issn.1672-4992.2006.09.009 .

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

单细胞凝胶电泳技术检测碳离子束和X射线照射对Lewis肺癌细胞的DNA损伤

淋巴细胞与单核细胞比值在中晚期胸部肿瘤放疗患者的预后相关性分析

多模态影像技术在优化头颈鳞癌微小淋巴结放疗中的应用

一种改进型放射外科准直器的设计和研究

兰州百合多糖片段对X射线诱导的小鼠骨髓有核细胞损伤的保护作用