电子束辐照对多酚氧化酶活性及结构的影响

顾可飞

doi:10.11889/j.1000-3436.2017.rrj.35.030401

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电子束辐照对多酚氧化酶活性及结构的影响

辐射技术的应用 | 更新时间：2021-12-10

- 电子束辐照对多酚氧化酶活性及结构的影响
- Effects of electron beam irradiation on the activity and structure of tyrosine oxidase
- 辐射研究与辐射工艺学报 2017年35卷第3期
- 作者机构：
  
  1.上海市农业科学院农产品质量标准与检测技术研究所上海 201304
  2.农业部农产品质量安全风险评估实验室上海 201304
  3.农业部食用菌监督检验测试中心上海 201304
- 作者简介：
  
  [ "顾可飞, 女, 1977年10月出生, 2007年于中国农业科学院研究生院获硕士学位, 目前为上海市农业科学院农产品质量标准与检测技术研究所助理研究员, 主要从事食品营养与安全控制, E-mail:xyz694@163.com", "GU Kefei (female) was born in 1977, and received her master's degree from Graduate School of Chinese Academy of Agricultural Sciences. Now she worked at Institute for Agri-food Quality Standards and Technology SAAS, Shanghai as a research assistant, focusing on food nutrition and safety control, E-mail:xyz694@163.com" ]
- 基金信息：
  
  上海市科技兴农重点攻关项目子课题(沪农科攻字（2009）第6-1号);农业部国家农产品质量安全风险评估项目“食用菌产品特质性营养品质评价与关键控制点评估(GJFP201700604)
- DOI：10.11889/j.1000-3436.2017.rrj.35.030401
  中图分类号：
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顾可飞. 电子束辐照对多酚氧化酶活性及结构的影响[J]. 辐射研究与辐射工艺学报, 2017,35(3):030401-6.

Kefei GU. Effects of electron beam irradiation on the activity and structure of tyrosine oxidase[J]. Journal of Radiation Research and Radiation Processing, 2017,35(3):030401-6.
顾可飞. 电子束辐照对多酚氧化酶活性及结构的影响[J]. 辐射研究与辐射工艺学报, 2017,35(3):030401-6. DOI： 10.11889/j.1000-3436.2017.rrj.35.030401.

Kefei GU. Effects of electron beam irradiation on the activity and structure of tyrosine oxidase[J]. Journal of Radiation Research and Radiation Processing, 2017,35(3):030401-6. DOI： 10.11889/j.1000-3436.2017.rrj.35.030401.

摘要

应用圆二色谱及SDS-PAGE技术分析电子束辐照对多酚氧化酶（Polyphenol oxidase，PPO）活性及结构的影响。结果发现：电子束辐照可在一定程度上降低PPO酶活性；圆二色谱分析发现电子束辐照处理后，PPO酶蛋白质二级结构相对含量发生改变，但α螺旋、β折叠和β转角总含量在吸收剂量 < 4.83 kGy时总量变化不大，当吸收剂量>4.83 kGy时，随着剂量的增加含量不断下降，无规则卷曲呈上升趋势。SDS-PAGE电泳实验结果表明，辐照处理下PPO酶同时发生交联和降解反应。上述结果提示电子束辐照对PPO酶活性有一定的抑制作用。

Abstract

The influence of electron beam irradiation on the activity and structure of PPO (Polyphenol oxidase) was analyzed. The results showed that electron beam irradiation can reduce PPO enzyme activity to a certain extent at a dose lower than 4.83 kGy. Analysis by circular dichroism (CD) showed that PPO protein's relative content of secondary structure affected by electron beam irradiation and total content of alpha helix, beta, and beta folding angle changed slightly. In contrast, at a dose higher than 4.83 kGy, the total content of the alpha helix, beta folding, and beta angle declined, and the random curl content increased. SDS-PAGE results showed that simultaneous cross-linking and degradation of the PPO enzyme occurred during irradiation. The experimental results indicate that electron beam irradiation can inhibit the activity of PPO.

关键词

多酚氧化酶电子束辐照蛋白质结构

Keywords

Polyphenol oxidaseElectron beam irradiationProtein structure

references

夏秀华. 多酚氧化酶的研究进展[J]. 粮食与食品工业, 2013, 20(6): 53-60. DOI: http://d.wanfangdata.com.cn/Periodical/lsyspgy201306015http://d.wanfangdata.com.cn/Periodical/lsyspgy201306015.

XIA Xiuhua. Research progress on polyphenol oxidase[J]. Cereal & Food Industry, 2013, 20(6):53-60. DOI:http://d.wanfangdata.com.cn/Periodical/lsyspgy201306015http://d.wanfangdata.com.cn/Periodical/lsyspgy201306015.

刘芳 , 赵金红 , 朱明慧 , 等 . 多酚氧化酶结构及褐变机理研究进展 . 食品研究与开发 , 2015 . 36 (6 ):113 -119 . DOI:10.3969/j.issn.1005-6521.2015.06.032http://doi.org/10.3969/j.issn.1005-6521.2015.06.032 .

Fang LIU , Jinhong ZHAO , Minghui ZHU , 等 . Advances in research of the structure and browning mechanism of polyphenol oxidase . Food Research and Development , 2015 . 36 (6 ):113 -119 . DOI:10.3969/j.issn.1005-6521.2015.06.032http://doi.org/10.3969/j.issn.1005-6521.2015.06.032 .

耿胜荣 , 程薇 , 廖涛 , 等 . γ射线和电子束辐照对牛血清蛋白结构的影响 . 现代食品科技 , 2016 . 32 (12 ):331 -332 . http://www.cnki.com.cn/Article/CJFDTOTAL-GZSP201612033.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-GZSP201612033.htm, .

Shengrong GENG , Wei CHENG , Tao LIAO , 等 . Effects of γ rays and electron beam irradiation on bovine serum albumin structure . Modern Food Science and Technology , 2016 . 32 (12 ):331 -332 . http://www.cnki.com.cn/Article/CJFDTOTAL-GZSP201612033.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-GZSP201612033.htm, .

刘超 , 汪晓鸣 , 王宏 . 辐照对梨汁多酚氧化酶活性的抑制作用 . 核农学报 , 2006 . 20 (4 ):331 -332. .

Chao LIU , Xiaoming WANG , Hong WANG . Inhibition effect of radiation on PPO's activity in pear juice . Journal of Nuclear Agricultural Sciences , 2006 . 20 (4 ):331 -332 . DOI:10.3969/j.issn.1000-8551.2006.04.020http://doi.org/10.3969/j.issn.1000-8551.2006.04.020 .

张琥. 红外辐照下马铃薯片灭酶工艺优化及多酚氧化酶失活机理的研究[D]. 中国农业机械化科学研究院, 2012.

ZHANG Hu. Study on processing optimization of potato slice inactivation and inactivation mechanism of PPO in infrared radiation[D]. Chinese Academy of Agricultural Mechanization Sciences, 2012.

顾可飞. 辐照对虾过敏原生化性质及抗原性的影响[D]. 中国农业科学院, 2007.

GU Kefei. Effeet of irradiationon bio-chemistry and antigenic properties of allergen in shrimp[D]. Chinese Academy of Agricultural Sciences, 2007.

顾可飞 , 赵志辉 , 高美须 , 等 . 电子束辐照技术在食品安全控制中的应用 . 中国食物与营养 , 2008 . (3 ):11 -14. .

Kefei GU , Zhihui ZHAO , Meixu GAO , 等 . Application of electron beam irradiation on food safety control . Food and Nutrition in China , 2008 . (3 ):11 -14 . DOI:10.3969/j.issn.1006-9577.2008.03.003http://doi.org/10.3969/j.issn.1006-9577.2008.03.003 .

章银良 , 杨慧 , 安巧云 . 羟自由基诱导蛋白质氧化损伤的研究 . 食品与生物技术学报 , 2012 . 31 (3 ):313 -318. .

Yinliang ZHANG , Hui YANG , Qiaoyun AN . Study on protein oxidation induced by hydroxyl radicals . Journal of Food Science and Biotechnology , 2012 . 31 (3 ):313 -318 . DOI:10.3969/j.issn.1673-1689.2012.03.015http://doi.org/10.3969/j.issn.1673-1689.2012.03.015 .

牛思思, 汪建明, 贺雅欣, 等. 羟基自由基氧化对蛋清蛋白质结构的影响[J/OL]. 食品工业科技: 优先出版. (2016-12-30)[2017-3-14]. http://www.cnki.net/kcms/detail/11.1759.TS.20161230.0859.008.htmlhttp://www.cnki.net/kcms/detail/11.1759.TS.20161230.0859.008.html.

NIU Sisi, WANG Jianming, HE Yaxin, et al. Effect of hydroxyl radical oxidation system on structure of egg white protein[J/OL]. Science and Technology of Food Industry:First On Line. (2016-12-30)[2017-3-14]. http://www.cnki.net/kcms/detail/11.1759.TS.20161230.0859.008.htmlhttp://www.cnki.net/kcms/detail/11.1759.TS.20161230.0859.008.html.

顾可飞 , 高美须 , 李春红 , 等 . 辐照对虾过敏原生化性质的影响 . 核农学报 , 2007 . 21 (2 ):160 -163. .

Kefei GU , Meixu GAO , Chunhong LI , 等 . Effect of irradiation on biochemistry properties of shrimp allergen . Journal of Nuclear Agricultural Sciences , 2007 . 21 (2 ):160 -163 . DOI:10.3969/j.issn.1000-8551.2007.02.014http://doi.org/10.3969/j.issn.1000-8551.2007.02.014 .

张立敏. 电子束辐照技术对鱼类过敏原活性及质构的影响[D]. 中国海洋大学, 2013.

ZHANG Limin. Effects of electron beam irradiation on the activity and texture of fish allergen[D]. Chinese Marine University, 2013.

李文杰 . 酶的催化作用与酶活性中心 . 生命的化学(中国生物化学会通讯) , 1989 . (5 ):34 -36 . http://www.cnki.com.cn/Article/CJFDTOTAL-SMHX198905019.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-SMHX198905019.htm, .

Wenjie LI . The catalysis and the active center of enzyme . Chemistry of Life (Newsletter of the Chinese Society of Biochemistry) , 1989 . (5 ):34 -36 . http://www.cnki.com.cn/Article/CJFDTOTAL-SMHX198905019.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-SMHX198905019.htm, .

彭益强 , 邓峰 , 刘宇 , 等 . 富士苹果中多酚氧化酶活性的中心必需基团与抑制动力学 . 华侨大学学报(自然科学版) , 2012 . 33 (1 ):51 -54 . DOI:10.11830/ISSN.1000-5013.2012.01.0051http://doi.org/10.11830/ISSN.1000-5013.2012.01.0051.

Yiqiang PENG , Feng DENG , Yu LIU , 等 . Researches on essential groups of enzyme active site and inhibition kinetics of polyphenol oxidase from fuji apple . Journal of Huaqiao University(Natural Science) , 2012 . 33 (1 ):51 -54 . DOI:10.11830/ISSN.1000-5013.2012.01.0051http://doi.org/10.11830/ISSN.1000-5013.2012.01.0051.

彭益强 , 刘鹏 , 邓峰 , 等 . 源于马铃薯的多酚氧化酶活性中心必需基团组成与抑制机理 . 化工进展 , 2012 . 31 (2 ):406 -411 . http://www.cnki.com.cn/Article/CJFDTOTAL-HGJZ201202028.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-HGJZ201202028.htm, .

Yiqiang PENG , Peng LIU , Feng DENG , 等 . Enzyme active center essential groups composition and inhibitory mechanism research of polyphenol oxidase from potato . Chemical Industry and Engineering Progress , 2012 . 31 (2 ):406 -411 . http://www.cnki.com.cn/Article/CJFDTOTAL-HGJZ201202028.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-HGJZ201202028.htm, .

胡春和 . 多酚氧化酶的研究现状 . 中国高新技术企业 , 2009 . (3 ):73 -74. .

Chunhe HU . Research status of polyphenol oxidase . China High Technology Enterprises , 2009 . (3 ):73 -74 . DOI:10.3969/j.issn.1009-2374.2009.03.037http://doi.org/10.3969/j.issn.1009-2374.2009.03.037 .

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