N-乙酰基-S-烯丙基-L-半胱氨酸的合成及其抗辐射活性

牟感恩; 龙伟; 李园园; 于光允; 沈秀; 周则卫

doi:10.11889/j.1000-3436.2017.rrj.35.050201

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N-乙酰基-S-烯丙基-L-半胱氨酸的合成及其抗辐射活性

辐射生物学与医学 | 更新时间：2021-12-10

- N-乙酰基-S-烯丙基-L-半胱氨酸的合成及其抗辐射活性
- Synthesis and anti-irradiation activities of N-acetyl-S-allyl-L-cysteine
- 辐射研究与辐射工艺学报 2017年35卷第5期
- 作者机构：
  
  1.中国医学科学院北京协和医学院放射医学研究所天津 300192
- 作者简介：
  
  [ "牟感恩, 女, 1992年2月出生, 2015年7月毕业于山东中医药大学, 现为中国医学科学院放射医学研究所硕士研究生, 研究方向为辐射防护及增敏药物研究, E-mail:tfdedmgn@126.com", "MU Ganen (female) was born in February 1992, and graduated from Chinese Medicine College of Shandong Province in 2015, now she is a master candidate at Institute of Radiation Medicine, Chinese Academy of Medical Sciences, majoring in radiation protection food and drug research. E-mail:tfdedmgn@126.com" ]
  周则卫, 硕士, 研究员, E-mail:zhouzewei@irm-cams.ac.cnZHOU Zewei, master's degree, professor, E-mail:zhouzewei@irm-cams.ac.cn
- 基金信息：
  
  天津市应用基础与前沿技术研究计划项目(15JCZDJC33400)
- DOI：10.11889/j.1000-3436.2017.rrj.35.050201
  中图分类号：
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牟感恩, 龙伟, 李园园, 等. N-乙酰基-S-烯丙基-L-半胱氨酸的合成及其抗辐射活性[J]. 辐射研究与辐射工艺学报, 2017,35(5):050201-8.

Gan'en MU, Wei LONG, Yuanyuan LI, et al. Synthesis and anti-irradiation activities of N-acetyl-S-allyl-L-cysteine[J]. Journal of Radiation Research and Radiation Processing, 2017,35(5):050201-8.
牟感恩, 龙伟, 李园园, 等. N-乙酰基-S-烯丙基-L-半胱氨酸的合成及其抗辐射活性[J]. 辐射研究与辐射工艺学报, 2017,35(5):050201-8. DOI： 10.11889/j.1000-3436.2017.rrj.35.050201.

Gan'en MU, Wei LONG, Yuanyuan LI, et al. Synthesis and anti-irradiation activities of N-acetyl-S-allyl-L-cysteine[J]. Journal of Radiation Research and Radiation Processing, 2017,35(5):050201-8. DOI： 10.11889/j.1000-3436.2017.rrj.35.050201.

摘要

以L-半胱氨酸为原料，经取代和酰化两步反应合成目标产物N-乙酰基-S-烯丙基-L-半胱氨酸（N-acetyl-S-allyl-L-cysteine，NAc-SAC）。以,137,Cs γ射线照射至不同剂量的ICR小鼠作为研究对象，通过30 d存活率实验、血相和免疫系统实验、各项脏器指数以及外周血淋巴细胞彗星实验，研究目标化合物分别在200、400、600 mg/kg给药剂量下的抗辐射活性。ICR小鼠经伽马射线照射至致死剂量7.5 Gy后，其存活率由单纯照射对照组的41.6%，分别提高到NAc-SAC给药组的58.3%、50.0%和66.7%，平均存活天数明显延长。,137,Cs γ射线照射至亚致死剂量6.0 Gy后，NAc-SAC在不同程度上增加了受照小鼠的脾指数、脾结节数（CFU-S）、骨髓DNA含量和白细胞数。彗星实验中7.0 Gy伽马射线照射联合NAc-SAC给药组小鼠外周血淋巴细胞的尾长、尾矩、Olive尾矩和尾部DNA百分含量明显低于7.0 Gy伽马射线单纯照射组（,p,<, 0.01），提示NAc-SAC可明显降低辐射导致的DNA损伤。本研究表明，目标化合物NAc-SAC具有一定的抗辐射损伤作用。

Abstract

The N-acetyl-S-allyl-L-cysteine (NAc-SAC) was synthesized by substitution and acylation reactions with L-cysteine as raw materials. Experiments on blood and immune-organs indexes, and survival rates of ICR mice within 30 d after ,137,Csγ-rays irradiation at different doses, as well as the comet assay were carried out to investigate the anti-irradiation effects of NAc-SAC. The survival rates of mice irradiated at 7.5 Gy with the dose of NAc-SAC at 200 mg/kg, 400 mg/kg, and 600 mg/kg increased to 58.3%, 50.0% and 66.7%, respectively, and the average survival time was significantly prolonged compared with that in the control group. The spleen indexes, colony forming cell-spleen (CFU-S), percentages of DNA, number of white blood cells of mice irradiated at 6.0 Gy increased under the action of NAc-SAC. The tail length, tail moment, olive tail moment, and tail DNA percentage of peripheral blood lymphocytes of mice irradiated at 7.0 Gy combined with NAc-SAC were higher than those for the control group (,p,<, 0.05). To conclude, NAc-SAC has a protective effect against damage induced by irradiation.

关键词

N-乙酰基-S-烯丙基-L-半胱氨酸辐射防护剂小鼠抗辐射活性137Cs γ射线

Keywords

N-acetyl-S-allyl-L-cysteine (NAc-SAC)Radiation protection agentMiceAnti-radiation activity137Cs γ-rays

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