重离子束辐照诱变选育北冬虫夏草高产菌株

贾国军; 马振贵; 赵凤舞; 陆锡宏; 王桂荣

doi:10.11889/j.1000-3436.2017.rrj.35.060401

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重离子束辐照诱变选育北冬虫夏草高产菌株

辐射技术的应用 | 更新时间：2021-12-10

- 重离子束辐照诱变选育北冬虫夏草高产菌株
- Screening and mutation breeding of high-yield Cordyceps militaris via heavy ion irradiation
- 辐射研究与辐射工艺学报 2017年35卷第6期
- 作者机构：
  
  1.兰州职业技术学院生物工程系兰州 730070
  2.中国科学院近代物理研究所兰州 730000
  3.甘肃农业大学动物医学院兰州 730070
- 作者简介：
  
  [ "贾国军, 男, 1986年3月出生, 2011年6月于安徽农业大学获硕士学位, 现为兰州职业技术学院讲师, 从事食品微生物与食药用菌领域的教学、科研及育种技术工作, E-mail:jiaguojun@lvu.edu.cn", "JIA Guojun (male) was born in March 1986, and graduated from Anhui Agricultural University in June 2011. Now he is a lecturer in Lanzhou Vocational Technical College, focusing on study and breeding techniques in the field of food microbiology and edible and medicinal fungi. E-mail:jiaguojun@lvu.edu.cn" ]
  赵凤舞, 博士, 教授, 主要从事生物医药和微生物资源的开发与应用工作, E-mail:zhaofengwu@lvu.edu.cnPh.D. ZHAO Fengwu, professor, engaged in the development and application of biological medicine and microbial resources. E-mail:zhaofengwu@lvu.edu.cn
- 基金信息：
  
  兰州市人才创新创业项目(2015-RC-21);兰州市科技计划项目(2016-3-2);兰州职业技术学院院内项目(2016B-1)
- DOI：10.11889/j.1000-3436.2017.rrj.35.060401
  中图分类号：
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贾国军, 马振贵, 赵凤舞, 等. 重离子束辐照诱变选育北冬虫夏草高产菌株[J]. 辐射研究与辐射工艺学报, 2017,35(6):060401-7.

Guojun JIA, Zhengui MA, Fengwu ZHAO, et al. Screening and mutation breeding of high-yield Cordyceps militaris via heavy ion irradiation[J]. Journal of Radiation Research and Radiation Processing, 2017,35(6):060401-7.
贾国军, 马振贵, 赵凤舞, 等. 重离子束辐照诱变选育北冬虫夏草高产菌株[J]. 辐射研究与辐射工艺学报, 2017,35(6):060401-7. DOI： 10.11889/j.1000-3436.2017.rrj.35.060401.

Guojun JIA, Zhengui MA, Fengwu ZHAO, et al. Screening and mutation breeding of high-yield Cordyceps militaris via heavy ion irradiation[J]. Journal of Radiation Research and Radiation Processing, 2017,35(6):060401-7. DOI： 10.11889/j.1000-3436.2017.rrj.35.060401.

摘要

利用,12,C,6+,重离子束对北冬虫夏草出发菌株的菌悬液进行辐照诱变处理，采用平板分离法初筛变异菌株，通过培养子实体复筛高产菌株，并测定子实体的产量、多糖和虫草素含量。结果表明，在吸收剂量为150Gy时，出发菌株的致死率达到最高为82%；经过初筛和复筛，最终选育出3株高产子实体突变菌株G2、G5、G12，并经连续传代10次，测定子实体产量稳定在8.02~8.21 g/瓶，与出发菌株的子实体产量相比提高了52.47%~56.08%，且稳定性保持良好。研究表明，利用重离子束辐照诱变选育北冬虫夏草，可以获得高产子实体菌株。

Abstract

Strains of ,Cordyceps militaris, grown in suspension culture were irradiated by ,12,C,6+, ion beam, screened using the plate separation method, and re-screened by culturing fruiting bodies to determine the fruiting-body yield, polysaccharide and cordycepin content. The strains displayed the highest lethality,i.e,., 82%, at the optimal absorbed dose of 150 Gy. Three mutants (G2, G5, G12) were obtained, which could produce a greater number of fruiting bodies. Meanwhile, the ten times pass-generation test indicated that the fruiting bodies stabilized at 8.02 to 8.21 g/bottle in these three mutants, which were 52.47% to 56.08% higher than the original strain, and the hereditary characters of these mutants were stable. The present findings show that a high-yield fruiting body of ,C.militaris, can be obtained by mutation breeding using a heavy ion beam irradiation.

关键词

北冬虫夏草重离子束诱变育种辐照

Keywords

Cordyceps militarisHeavy ion beamsMutation breedingIrradiation

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