快中子辐照玉米自交系的细胞学效应及后代变异

刘忠祥; 徐大鹏; 连晓荣; 周文期; 寇思荣; 姚泽恩

doi:10.11889/j.1000-3436.2017.rrj.35.060402

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快中子辐照玉米自交系的细胞学效应及后代变异

辐射技术的应用 | 更新时间：2021-12-10

- 快中子辐照玉米自交系的细胞学效应及后代变异
- Cytological effects and offspring variation of maize inbred lines irradiated by fast neutron
- 辐射研究与辐射工艺学报 2017年35卷第6期
- 作者机构：
  
  1.甘肃省农业科学院作物研究所兰州 730070
  2.兰州大学核科学与技术学院兰州 730000
  3.兰州大学教育部中子应用技术工程研究中心兰州 730000
- 作者简介：
  
  [ "刘忠祥, 男, 1964年7月出生, 2008年毕业于甘肃农业大学, 现为甘肃省农业科学院作物研究所副研究员", "LIU Zhongxiang (male) was born in July 1964, and received his bachelor degree from Gansu Agriculture University in 2008. Now he is an associate research fellow of Crops Research Institute, Gansu Academy of Agricultural Sciences" ]
  姚泽恩, 博士, 教授, E-mail:zeyao@lzu.edu.cnPh.D. YAO Ze'en, professor, E-mail:zeyao@lzu.edu.cn
- 基金信息：
  
  国家重点研发计划(2016YFD0100103-19);甘肃省农科院农业科技创新专项(2011GAAS07);兰州大学中央高校基本科研业务费专项资金(lzujbky-2015-bt07)
- DOI：10.11889/j.1000-3436.2017.rrj.35.060402
  中图分类号：
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刘忠祥, 徐大鹏, 连晓荣, 等. 快中子辐照玉米自交系的细胞学效应及后代变异[J]. 辐射研究与辐射工艺学报, 2017,35(6):060402-8.

Zhongxiang LIU, Dapeng XU, Xiaorong LIAN, et al. Cytological effects and offspring variation of maize inbred lines irradiated by fast neutron[J]. Journal of Radiation Research and Radiation Processing, 2017,35(6):060402-8.
刘忠祥, 徐大鹏, 连晓荣, 等. 快中子辐照玉米自交系的细胞学效应及后代变异[J]. 辐射研究与辐射工艺学报, 2017,35(6):060402-8. DOI： 10.11889/j.1000-3436.2017.rrj.35.060402.

Zhongxiang LIU, Dapeng XU, Xiaorong LIAN, et al. Cytological effects and offspring variation of maize inbred lines irradiated by fast neutron[J]. Journal of Radiation Research and Radiation Processing, 2017,35(6):060402-8. DOI： 10.11889/j.1000-3436.2017.rrj.35.060402.

摘要

为了探索快中子辐照玉米的细胞学效应及后代变异，为玉米种质创新及品种改良提供有益的种质资源，本实验采用低剂量（0.36~4.19 Gy）的快中子辐照玉米自交系LY8405和PH6WC干种子。结果表明：两个自交系根尖细胞有丝分裂指数均随吸收剂量的增加而降低；核畸变率随吸收剂量的增加而升高；吸收剂量4.19Gy对自交系LY8405的M,1,染色体分裂行为影响效应最为明显，2.48 Gy对自交系PH6WC的M,1,染色体分裂行为影响效应次之；辐照M,2,、M,3,代突变类型包括株型、叶色、穗形、株高、籽粒形状及大小等；生物学和细胞学效应表明，2.48~4.19 Gy的吸收剂量是0.36~4.19 Gy范围内快中子辐照选育玉米突变体的适宜剂量。

Abstract

Dry seeds of maize inbred lines LY8405 and PH6WC were irradiated by fast neutron at doses of 0.36~4.19 Gy to explore the cytological effect of irradiation on maize for germplasm innovation and improvement. The results showed that with the increase of absorbed doses, the mitotic index of root tip cells decreased, while the rate of nuclear aberration increased. The chromosome division behavior of irradiated inbred lines LY8405 in M,1, generation was the most obvious at dose of 4.19 Gy, followed by the irradiated inbred lines PH6WC at dose of 2.48 Gy. Abundant mutation types appeared in M,2, and M,3, generation of inbred lines LY8405 and PH6WC under fast neutron irradiation, including those of plant shape, leaf color, panicle length, plant height, grain shape, and grain size,etc,. Biological and cytological studies show that with maize seed irradiated by fast neutron at 0.36~4.19 Gy, the appropriate dose range for breeding is 2.48~4.19 Gy.

关键词

玉米快中子辐照细胞学效应后代变异分析

Keywords

MaizeFast neutron irradiationCytological effectProgeny variation analysis

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