辐射诱变结合抗自身产物选育替考拉宁高产菌株

张引; 张祝兰; 邱观荣; 杨煌建; 王德森; 任林英; 连云阳

doi:10.11889/j.1000-3436.2018.rrj.36.010401

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辐射诱变结合抗自身产物选育替考拉宁高产菌株

辐射技术的应用 | 更新时间：2021-12-10

- 辐射诱变结合抗自身产物选育替考拉宁高产菌株
- Screening for high teicoplanin-producing strain by mutation combined with resistance to self-produced metabolite
- 辐射研究与辐射工艺学报 2018年36卷第1期页码：10401
- 作者机构：
  
  1.福建省微生物研究所福建省新药(微生物)筛选重点实验室福州 350007
- 作者简介：
  
  [ "张引, 男, 1985年12月出生, 2007年毕业于福建师范大学, 现为福建省微生物研究所助理研究员, 研究方向为微生物发酵, E-mail:tbsbt@163.com", "ZHANG Yin (male) was born in December 1985, and graduated from Fujian Normal University in 2007. Now he is a assistant professor in Fujian Institute of Microbiology, majoring in microbiology fermentation, E-mail: tbsbt@163.com" ]
  张祝兰, 研究员, E-mail:jessylan9963@sina.comZHANG Zhulan, professor, E-mail:jessylan9963@sina.com
  连云阳, 博士, 研究员, E-mail:ylianfim@139.comPh.D. LIAN Yunyang, professor, E-mail: ylianfim@139.com
- 基金信息：
  
  福建省科技重大项目(2014YZ0004-2);福建省化药技术重大研发平台(2014Y2001);福州市生物制药行业技术创新中心(2016-PT-36);福建省公益类科研院所专项(2017R1009-13)
- DOI：10.11889/j.1000-3436.2018.rrj.36.010401
  中图分类号：
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张引, 张祝兰, 邱观荣, 等. 辐射诱变结合抗自身产物选育替考拉宁高产菌株[J]. 辐射研究与辐射工艺学报, 2018,36(1):10401.

Yin ZHANG, Zhulan ZHANG, Guanrong QIU, et al. Screening for high teicoplanin-producing strain by mutation combined with resistance to self-produced metabolite[J]. Journal of Radiation Research and Radiation Processing, 2018,36(1):10401.
张引, 张祝兰, 邱观荣, 等. 辐射诱变结合抗自身产物选育替考拉宁高产菌株[J]. 辐射研究与辐射工艺学报, 2018,36(1):10401. DOI： 10.11889/j.1000-3436.2018.rrj.36.010401.

Yin ZHANG, Zhulan ZHANG, Guanrong QIU, et al. Screening for high teicoplanin-producing strain by mutation combined with resistance to self-produced metabolite[J]. Journal of Radiation Research and Radiation Processing, 2018,36(1):10401. DOI： 10.11889/j.1000-3436.2018.rrj.36.010401.

摘要

采用,60,Co γ-射线辐射结合抗性筛选技术，对替考拉宁产生菌替考游动放线菌FIM-16的孢子进行诱变处理，以替考拉宁自身抗性作为选择压力，并通过摇瓶发酵对抗性基因突变株进行筛选。结果获得一株摇瓶发酵水平较出发菌株FIM-16提高两倍的高产菌FIM-16-58，且遗传性状相对稳定，菌种传3代发酵水平无明显影响，菌种斜面在4 ℃稳定保藏两个月；其在1 T发酵罐上发酵，添加0.1%的玉米油可有效抑制泡沫的产生，替考拉宁产量达5.0 g/L以上。

Abstract

To screen high-yield teicoplanin-producing strain,60,Co γ-ray irradiated spore suspension of the strain ,Actinoplanes teichomyceticus, FIM-16 was spread on regenerating plate containing lethal concentration of teicoplanin. Teicoplanin-resistant mutants were picked and screened by shake-flask fermentation. Strain FIM-16-58 showed two times higher teicoplanin yield than the original strain, and demonstrated higher hereditary stability. There was no obvious effect on the fermentation level of the strain over three generations, and its slope was stable at 4 ℃ for two months. During scale-up experiment in the 1 T fermenter, foam was suppressed effectively by adding 0.1% corn oil, and the yield of teicoplanin reached over 5.0 g/L. Therefore, the high-yield strain laid the foundation of its industrial production and fermentation.

关键词

替考游动放线菌抗性突变筛选辐射诱变替考拉宁

Keywords

Actinoplanes teichomyceticusResistance mutation screeningRadiation mutagenesisTeicoplanin

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