复方树莓籽粉对辐照小鼠骨髓细胞的防护作用

喻培勋; 苑伟; 孟庆兰

doi:10.11889/j.1000-3436.2018.rrj.36.020202

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复方树莓籽粉对辐照小鼠骨髓细胞的防护作用

辐射生物学与医学 | 更新时间：2021-12-10

- 复方树莓籽粉对辐照小鼠骨髓细胞的防护作用
- Protective effect of compound raspberry seed powder on bone marrow cells of irradiated mice
- 辐射研究与辐射工艺学报 2018年36卷第2期
- 作者机构：
  
  1.青岛大学医学院人体解剖学教研室青岛 266021
  2.青岛大学生命科学学院青岛 266021
- 作者简介：
  
  [ "喻培勋, 男, 1992年出生, 2016年获湖北中医药大学学士学位, 现青岛大学硕士研究生在读, 主要从事临床应用解剖学, E-mail:1634436987@qq.com", "YU Peixun (male) was born in 1992, and received his bachelor's degree from the Hubei University of Traditional Chinese Medicine in 2016. Now he is a master candidate in Qingdao University, majoring in clinical applied anatomy. E-mail:1634436987@qq.com" ]
  孟庆兰, 教授, 硕士生导师, E-mail:13791910793@163.com MENG Qinglan, professor, master's tutor, E-mail: 13791910793@163.com
- 基金信息：
- DOI：10.11889/j.1000-3436.2018.rrj.36.020202
  中图分类号：
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喻培勋, 苑伟, 孟庆兰. 复方树莓籽粉对辐照小鼠骨髓细胞的防护作用[J]. 辐射研究与辐射工艺学报, 2018,36(2):020202-7.

Peixun YU, Wei YUAN, Qinglan MENG. Protective effect of compound raspberry seed powder on bone marrow cells of irradiated mice[J]. Journal of Radiation Research and Radiation Processing, 2018,36(2):020202-7.
喻培勋, 苑伟, 孟庆兰. 复方树莓籽粉对辐照小鼠骨髓细胞的防护作用[J]. 辐射研究与辐射工艺学报, 2018,36(2):020202-7. DOI： 10.11889/j.1000-3436.2018.rrj.36.020202.

Peixun YU, Wei YUAN, Qinglan MENG. Protective effect of compound raspberry seed powder on bone marrow cells of irradiated mice[J]. Journal of Radiation Research and Radiation Processing, 2018,36(2):020202-7. DOI： 10.11889/j.1000-3436.2018.rrj.36.020202.

摘要

探讨复方树莓籽粉水溶液对急性放射性损伤小鼠骨髓细胞的影响及防护作用。建立小鼠急性放射性损伤模型，设立空白对照组、模型对照组及不同浓度复方树莓籽粉溶液处理组（低/中/高剂量组），观察辐照后各组小鼠骨髓细胞有核细胞数、微核率、细胞周期及线粒体膜电位的变化情况。实验结果表明，与空白对照组比较，模型对照组变化均非常显著（,p,<, 0.01）。与模型对照组比较，复方树莓籽粉中、高剂量组有核细胞数分别升高78.2%（,p,<, 0.01）、116.9%（,p,<, 0.01）；高剂量组微核率减少27.3%（,p,<, 0.01）；中剂量组细胞周期G1期、S期，高剂量组S期细胞比例升高（,p,<, 0.05），高剂量组G1期细胞比例显著升高（,p,<, 0.01）；中、高剂量组骨髓细胞线粒体膜电位显著升高（,p,<, 0.01）。实验结果提示，复方树莓籽粉能升高骨髓细胞数，降低骨髓细胞微核率，升高骨髓细胞线粒体膜电位，减少骨髓细胞周期阻滞，抑制辐射损伤骨髓细胞的细胞凋亡，可能对辐照导致的骨髓细胞损伤有防护作用。

Abstract

This study explores the protective effect of compound raspberry seed powder on the bone marrow cells of acute radiation-injured mice. In the experiment, an acute radiation injury model was generated in mice. A blank control group, a model control group, and treatment groups of different concentrations of compound raspberry seed powder solution (low/medium/high dose group) were established. After irradiation, the changes in nucleus cells, micronucleus rate, cell cycle, and mitochondrial membrane potential of mice bone marrow cells were measured. Compared with the blank control group, the model control group and treatment groups were significantly different (,p,<, 0.01).Compared with the model control group, the number of nucleated cells increased by 78.2% (,p,<, 0.01) and 116.9% (,p,<, 0.01), respectively, in the medium, and high dose groups, and the micronucleus rate in the high dose group decreased by 27.3% (,p,<, 0.01). The proportion of cells in the G1 phase and S phase in the medium dose group and in the S phase of high dose group was increased (,p,<, 0.05). The proportion of cells in the G1 phase of the high dose group increased significantly (,p,<, 0.01). The mitochondrial membrane potential of bone marrow cells in the medium and high dose groups was significantly increased (,p,<, 0.01). The results indicated that compound raspberry seed powder increased the number of bone marrow cells, reduced the micronucleus rate, increased the mitochondrial membrane potential, reduced cell cycle arrest, and inhibited the induction of apoptosis by irradiation damage in bone marrow cells, which suggested that compound raspberry seed powder may play an important role in the protection of bone marrow cells injured by irradiation.

关键词

急性放射性损伤复方树莓籽粉骨髓细胞损伤凋亡辐射防护

Keywords

Acute radiation injuryCompound raspberry seed powderBone marrow cellsInjuryApoptosisRadiation protection

references

张广慧 , 吴红英 , 靳瑾 , 等 . 植物凝集素PHA-L对小鼠急性辐射防护作用 . 辐射研究与辐射工艺学报 , 2016 . 34 (2 ):020203 DOI:10.11889/j.1000-3436.2016.rrj.34.020203http://doi.org/10.11889/j.1000-3436.2016.rrj.34.020203 http://www.j.sinap.ac.cn/fushe/CN/abstract/abstract297.shtmlhttp://www.j.sinap.ac.cn/fushe/CN/abstract/abstract297.shtml, .

Guanghui ZHANG , Hongying WU , Jin JIN , 等 . Protective effects of the plant lectin PHA-L on acute radiation injuries in mice . Journal of Radiation Research and Radiation Processing , 2016 . 34 (2 ):020203 DOI:10.11889/j.1000-3436.2016.rrj.34.020203http://doi.org/10.11889/j.1000-3436.2016.rrj.34.020203 http://www.j.sinap.ac.cn/fushe/CN/abstract/abstract297.shtmlhttp://www.j.sinap.ac.cn/fushe/CN/abstract/abstract297.shtml, .

王淑琴 . 电离辐射对人类健康的影响 . 中国医学装备 , 2007 . 4 (9 ):11 -14 . DOI:10.3969/j.issn.1672-8270.2007.09.004http://doi.org/10.3969/j.issn.1672-8270.2007.09.004 .

Shuqin WANG . Effects of ionizing radiation on human health . Chinese Medical Equipment , 2007 . 4 (9 ):11 -14 . DOI:10.3969/j.issn.1672-8270.2007.09.004http://doi.org/10.3969/j.issn.1672-8270.2007.09.004 .

I R Vogelius , S M Bentzen . A literature-based meta-analysis of clinical risk factors for development of radiation induced pneumonitis . Acta Oncologica , 2012 . 51 (8 ):975 DOI:10.3109/0284186X.2012.718093http://doi.org/10.3109/0284186X.2012.718093 .

M Fay , C M Poole , G Pratt . Recent advances in radiotherapy for thoracic tumours . Journal of Thoracic Disease , 2013 . 5 (4 ):S551 -S555 . DOI:10.1200/JOP.2016.019711http://doi.org/10.1200/JOP.2016.019711 .

王祯祯 , 王海涛 , 高光凯 , 等 . 复方树莓籽粉对急性放射性损伤作用的研究 . 中华放射医学与防护杂志 , 2017 . 37 (1 ):24 -28 . DOI:10.3760/cma.j.issn.0254-5098.2017.01.005http://doi.org/10.3760/cma.j.issn.0254-5098.2017.01.005 .

Zhenzhen WANG , Haitao WANG , Guangkai GAO , 等 . Compound raspberry seed powder on acute radiation injury research . Chinese Journal of Radiation Medicine and Protection , 2017 . 37 (1 ):24 -28 . DOI:10.3760/cma.j.issn.0254-5098.2017.01.005.issn.0254-5098.2017.01.005http://doi.org/10.3760/cma.j.issn.0254-5098.2017.01.005.issn.0254-5098.2017.01.005 .

柏春玲 , 廖泽容 , 张巧 , 等 . 应用流式细胞术快速测定细胞周期的DNA一步法 . 昆明医科大学学报 , 2017 . 38 (2 ):10 -13 . DOI:10.3969/j.issn.1003-4706.2017.02.003http://doi.org/10.3969/j.issn.1003-4706.2017.02.003 .

Chunling BAI , Zerong LIAO , Qiao ZHANG , 等 . DNA one-step method for rapid cell cycle determination by flow cytometry . Journal of Kunming Medical University , 2017 . 38 (2 ):10 -13 . DOI:10.3969/j.issn.1003-4706.2017.02.003http://doi.org/10.3969/j.issn.1003-4706.2017.02.003 .

胡永亮 , 谭启杏 , 王红丽 , 等 . Sestrin2通过抑制砷化物诱导的氧化应激反应发挥拮抗细胞凋亡的保护性作用 . 生物技术通讯 , 2016 . 27 (3 ):314 -317 . DOI:10.3969/j.issn.1009-0002.2016.03.003http://doi.org/10.3969/j.issn.1009-0002.2016.03.003 .

Yongliang HU , Qixing TAN , Hongli WANG , 等 . Sestrin2 play a protective role against apoptosis by inhibiting arsenic induced oxidative stress . Biotechnology Communication , 2016 . 27 (3 ):314 -317 . DOI:10.3969/j.issn.1009-0002.2016.03.003http://doi.org/10.3969/j.issn.1009-0002.2016.03.003 .

孔越 , 王庆辉 , 尚明英 , 等 . 小叶莲有效成分对人乳腺癌细胞增殖、细胞周期及线粒体膜电位的影响 . 中国药房 , 2017 . 28 (10 ):1368 -1371 . DOI:10.6039/j.issn.1001-0408.2017.10.19http://doi.org/10.6039/j.issn.1001-0408.2017.10.19 .

Yue KONG , Qinghui WANG , Mingying SHANG , 等 . Effect on the proliferation, cell cycle and mitochondrial membrane potential of human breast cancer cells . Chinese Pharmacy , 2017 . 28 (10 ):1368 -1371 . DOI:10.6039/j.issn.1001-0408.2017.10.19http://doi.org/10.6039/j.issn.1001-0408.2017.10.19 .

佟晓波 , 朱长林 , 牛嗣云 . 母乳中抗氧化物质维生素E的研究近况 . 承德医学院学报 , 2002 . 19 (3 ):239 -241 . DOI:10.3969/J.ISSN.1004-6879.2002.03.047http://doi.org/10.3969/J.ISSN.1004-6879.2002.03.047 .

Xiaobo TONG , Changlin ZHU , Siyun NIU . Recent research on antioxidant vitamin E in breast milk . Journal of Chengde Medical College , 2002 . 19 (3 ):239 -241 . DOI:10.3969/J.ISSN.1004-6879.2002.03.047http://doi.org/10.3969/J.ISSN.1004-6879.2002.03.047 .

朱长林 , 佟晓波 , 张小华 , 等 . 不同阶段母乳中a-生育酚浓度的研究 . 中国实用儿科杂志 , 2002 . 17 (10 ):624 -625 . DOI:10.3969/J.ISSN.1005-2224.2002.10.012http://doi.org/10.3969/J.ISSN.1005-2224.2002.10.012 .

Changlin ZHU , Xiaobo TONG , Xiaohua ZHANG , 等 . A study of the concentration of alpha tocopherol in different stages of breast milk . Chinese Journal of Practical Pediatrics , 2002 . 17 (10 ):624 -625 . DOI:10.3969/J.ISSN.1005-2224.2002.10.012http://doi.org/10.3969/J.ISSN.1005-2224.2002.10.012 .

B K Yoon , Y H Kang , W J Oh , 等 . Impact of lysophosphatidylcholine on the plasminogen activator system in cultured vascular smooth muscle cells . Journal of Korean Medical Science , 2012 . 27 (7 ):803 -810 . DOI:10.3346/jkms.2012.27.7.803http://doi.org/10.3346/jkms.2012.27.7.803 .

K C Jeng , C S Yang , W Y Siu , 等 . Supplementation with vitamins C and E enhances cytokine production by peripheral blood mononuclear cells in healthy adults . American Journal of Clinical Nutrition , 1996 . 64 (6 ):960 -965 . DOI:10.1093/ajcn/64.6.960http://doi.org/10.1093/ajcn/64.6.960.

剧红梅 , 曲梓怡 , 王召令 , 等 . 原花青素的抗氧化作用 . 中国组织工程研究 , 2007 . 11 (16 ):3155 -3157 . DOI:10.3321/j.issn:1673-8225.2007.16.057http://doi.org/10.3321/j.issn:1673-8225.2007.16.057 .

Hongmei JU , Ziyi QU , Zhaoling WANG , 等 . The antioxidant effects of procyanidins . China Tissue Engineering Research , 2007 . 11 (16 ):3155 -3157 . DOI:10.3321/j.issn:1673-8225.2007.16.057http://doi.org/10.3321/j.issn:1673-8225.2007.16.057 .

韩晓亮 , 蒲国华 , 李宝永 , 等 . 丙泊酚对电离辐射引起小鼠造血系统损伤的防护作用 . 国际药学研究杂志 , 2016 . 43 (5 ):931 -934 . DOI:10.13220/j.cnki.jipr.2016.05.023http://doi.org/10.13220/j.cnki.jipr.2016.05.023 .

Xiaoliang HAN , Guohua PU , Baoyong LI , 等 . Propofol protect against hematopoietic system damage induced by ionizing radiation . International Journal of Pharmaceutical Research , 2016 . 43 (5 ):931 -934 . DOI:10.13220/j.cnki.jipr.2016.05.023http://doi.org/10.13220/j.cnki.jipr.2016.05.023 .

李园园 , 张宇睿 , 徐文清 . 一氧化氮清除剂Fe(DETC)2对小鼠的辐射防护作用 . 辐射研究与辐射工艺学报 , 2017 . 35 (4 ):040203 DOI:10.11889/j.1000-3436.2017.rrj.35.040302http://doi.org/10.11889/j.1000-3436.2017.rrj.35.040302 http://www.j.sinap.ac.cn/fushe/CN/abstract/abstract420.shtmlhttp://www.j.sinap.ac.cn/fushe/CN/abstract/abstract420.shtml, .

Yuanyuan LI , Yurui ZHANG , Wenqing XU . Radio-protective effects of a nitric oxide scavenger, Fe(DETC)2, on mice . Journal of Radiation Research and Radiation Processing , 2017 . 35 (4 ):040203 DOI:10.11889/j.1000-3436.2017.rrj.35.040302http://doi.org/10.11889/j.1000-3436.2017.rrj.35.040302 http://www.j.sinap.ac.cn/fushe/CN/abstract/abstract420.shtmlhttp://www.j.sinap.ac.cn/fushe/CN/abstract/abstract420.shtml, .

C A Vidair , C H Chen , C C Ling , 等 . Apoptosis induced by X-irradiation of rec-myc cells is postmitotic and not predicted by the time after irradiation or behavior of sister cells . Cancer Research , 1996 . 56 (18 ):4116 -4118 . https://www.researchgate.net/publication/14404867_Apoptosis_Induced_by_X-Irradiation_of_rec-myc_Cells_Is_Postmitotic_and_not_Predicted_by_the_Time_after_Irradiation_or_Behavior_of_Sister_Cells1https://www.researchgate.net/publication/14404867_Apoptosis_Induced_by_X-Irradiation_of_rec-myc_Cells_Is_Postmitotic_and_not_Predicted_by_the_Time_after_Irradiation_or_Behavior_of_Sister_Cells1, .

J M Michael , M F Lavin , D J Watters . Resistance to radiation-induced apoptosis in Burkitt's lymphoma cells is associated with defective ceramide signaling . Cancer Research , 1997 . 57 (16 ):3600 -3605 . https://www.researchgate.net/profile/Martin_Lavin2/publication/13950368_Resistance_to_radiation-induced_apoptosis_in_Burkitt%27s_Lymphoma_cells_is_associated_with_defective_ceramide_signaling/links/556cf5fc08aefcb861d7e920/Resistance-to-radiation-induced-apoptosis-in-Burkitts-Lymphoma-cells-is-associated-with-defective-ceramide-signaling.pdfhttps://www.researchgate.net/profile/Martin_Lavin2/publication/13950368_Resistance_to_radiation-induced_apoptosis_in_Burkitt%27s_Lymphoma_cells_is_associated_with_defective_ceramide_signaling/links/556cf5fc08aefcb861d7e920/Resistance-to-radiation-induced-apoptosis-in-Burkitts-Lymphoma-cells-is-associated-with-defective-ceramide-signaling.pdf, .

张晓峰 , 李百祥 , 张旸 , 等 . 神经酰胺以Caspase依赖和非依赖方式诱导线粒体凋亡蛋白释放 . 中国科学 , 2008 . 38 (2 ):109 -114 . http://www.oalib.com/paper/4130229http://www.oalib.com/paper/4130229, .

Xiaofeng ZHANG , Baixiang LI , Yang ZHANG , 等 . Ceramide induces mitochondrial apoptotic protein release by Caspase dependent and non dependent manner . Chinese Science , 2008 . 38 (2 ):109 -114 . http://www.oalib.com/paper/4130229http://www.oalib.com/paper/4130229, .

杨宏 , 倪敏 , 赵心彬 , 等 . 辐射致组织损伤凋亡机制的研究概况 . 药学实践杂志 , 2014 . 32 (2 ):96 -97 . DOI:10.3969/j.issn.1006-0111.2014.02.005http://doi.org/10.3969/j.issn.1006-0111.2014.02.005 .

Hong YANG , Min NI , Xinbin ZHAO , 等 . Radiation induced mechanism of tissue injury apoptosis . Pharmaceutical Practice Journal , 2014 . 32 (2 ):96 -97 . DOI:10.3969/j.issn.1006-0111.2014.02.005http://doi.org/10.3969/j.issn.1006-0111.2014.02.005 .

刘冰 , 张俊伶 , 李德冠 , 等 . Vam3对小鼠体外胸腺细胞的辐射防护效应 . 辐射研究与辐射工艺学报 , 2016 . 34 (2 ):020207 DOI:10.11889/j.1000-3436.2016.rrj.34.020207http://doi.org/10.11889/j.1000-3436.2016.rrj.34.020207 http://www.j.sinap.ac.cn/fushe/CN/abstract/abstract274.shtmlhttp://www.j.sinap.ac.cn/fushe/CN/abstract/abstract274.shtml, .

Bing LIU , Junling ZHANG , Deguan LI , 等 . Radiation protection effects of Vam3 on mouse thymocyte in vitro . Journal of Radiation Research and Radiation Processing , 2016 . 34 (2 ):020207 DOI:10.11889/j.1000-3436.2016.rrj.34.020207http://doi.org/10.11889/j.1000-3436.2016.rrj.34.020207 http://www.j.sinap.ac.cn/fushe/CN/abstract/abstract274.shtmlhttp://www.j.sinap.ac.cn/fushe/CN/abstract/abstract274.shtml, .

梅寒芳 , 谢朝阳 , 祝其锋 . 原花青素对A β 25-35诱导PC12细胞凋亡的影响及其机制的研究 . 中国药理学通报 , 2005 . 21 (2 ):227 -231 . DOI:10.3321/j.issn:1001-1978.2005.02.026.MEIhttp://doi.org/10.3321/j.issn:1001-1978.2005.02.026.MEI .

Hanfang MEI , Zhaoyang XIE , Qifeng ZHU . The study of proanthocyanidins on the effects of A beta 25-35 induced apoptosis in PC12 cells and its mechanism . China Pharmacological Bulletin , 2005 . 21 (2 ):227 -231 . DOI:10.3321/j.issn:1001-1978.2005.02.026http://doi.org/10.3321/j.issn:1001-1978.2005.02.026 .

孙建仙 , 刘小立 . 神经酰胺的代谢及其在细胞凋亡中的作用 . 科技信息 , 2008 . (33 ):22 DOI:10.3969/j.issn.1001-9960.2008.33.013.SUNhttp://doi.org/10.3969/j.issn.1001-9960.2008.33.013.SUN .

Jianxian SUN , Xiaoli LIU . Metabolism of ceramide and its role in cell apoptosis . Science and Technology Information , 2008 . (33 ):22 DOI:10.3969/j.issn.1001-9960.2008.33.013http://doi.org/10.3969/j.issn.1001-9960.2008.33.013 .

莫冰. 原花青素对组蛋白诱导淋巴细胞凋亡的作用及机制[D]. 广州: 南方医科大学, 2015. DOI: 10.7666/d.Y2911186.http://dx.doi.org/10.7666/d.

MO Bing, The effect and mechanism of proanthocyanidins on histone induced lymphocyte apoptosis[D]. Guangzhou: Southern Medical University, 2015. DOI: 10.7666/d.Y2911186.http://dx.doi.org/10.7666/d.Y2911186.

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