西咪替丁对伽马射线辐照小鼠免疫功能的保护作用
Protective effect of cimetidine on immune function of mice irradiated by gamma-rays
- 辐射研究与辐射工艺学报 2018年36卷第2期
- 作者机构：
1.第二军医大学海军医学研究所 上海 200433
- 作者简介：
[ "何颖, 女, 1974年1月出生, 2008年于第二军医大学获得博士学位, 副研究员, 研究方向为辐射防护医学", "HE Ying (female) was born in January 1974, and received her doctor's degree from The Second Military Medical University in 2008. Now she is an associate researcher engaging in radiation protection medicine" ]
沈先荣, 博士, 研究员, E-mail:xianrong_sh@163.com Ph.D. SHEN Xianrong, researcher, E-mail: xianrong_sh@163.com
- 基金信息：家新药创制科技重大专项军队特需药品保密专项(2014ZX09J14103-07B)
DOI：10.11889/j.1000-3436.2018.rrj.36.020203
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何颖, 张俊玲, 赵忆宁, 等. 西咪替丁对伽马射线辐照小鼠免疫功能的保护作用[J]. 辐射研究与辐射工艺学报, 2018,36(2):020203-7.
Ying HE, Junling ZHANG, Yining ZHAO, et al. Protective effect of cimetidine on immune function of mice irradiated by gamma-rays[J]. Journal of Radiation Research and Radiation Processing, 2018,36(2):020203-7.
何颖, 张俊玲, 赵忆宁, 等. 西咪替丁对伽马射线辐照小鼠免疫功能的保护作用[J]. 辐射研究与辐射工艺学报, 2018,36(2):020203-7. DOI： 10.11889/j.1000-3436.2018.rrj.36.020203.
Ying HE, Junling ZHANG, Yining ZHAO, et al. Protective effect of cimetidine on immune function of mice irradiated by gamma-rays[J]. Journal of Radiation Research and Radiation Processing, 2018,36(2):020203-7. DOI： 10.11889/j.1000-3436.2018.rrj.36.020203.
摘要
雄性C57小鼠60只平均分为6组：正常对照组（0 Gy）、模型对照组（1.0 Gy）、阳性药对照组（1.0 Gy+523片）、西咪替丁（Cimetidine，CMD）低、中、高剂量组（1.0 Gy+33.3、1.0 Gy+100、1.0 Gy+300 mg/kg），采用,60,Co γ射线全身照射至吸收剂量1.0 Gy，剂量率为8.33 mGy/min。CMD于照射前连续灌胃给药6 d，每天1次，照射后5 h给药1次。照射后24 h检测脾脏淋巴细胞凋亡率、T淋巴细胞亚群、B淋巴细胞数和血清中IL-2、IL-4、IL-6、IL-12、IFN-γ和TNF-α含量。结果表明：与正常对照组比较，照射后24 h，小鼠脾脏淋巴细胞凋亡率显著增加（,p,<, 0.01），CD3,+,、CD3,+,CD8,+,T细胞和CD3,-,CD19,+,B细胞数量均下降（,p,<, 0.01），血清中的IL-4、IL-12和TNF-α含量显著降低（,p,<, 0.01，,p,<, 0.05）；与辐照模型组比较，CMD各剂量组脾脏淋巴细胞凋亡率显著下降（,p,<, 0.01），CMD给药组血清中IL-4、IL-6、IL-12、TNF-α和IFN-γ含量显著增加（,p,<, 0.01，,p,<, 0.05），CMD高剂量组的CD3,+,、CD3,+,CD4,+,和CD3,+,CD8,+,T细胞及CD3,-,CD19,+,B细胞数量均显著上升（,p,<, 0.01，,p,<, 0.05）。结果提示，CMD可有效改善γ射线辐照小鼠的免疫功能，具有较好的辐射保护作用。
Abstract
Sixty male mice were divided into six groups:normal control group (0 Gy), model control group (1.0 Gy), positive control group (1.0 Gy+523 tablet), and cimetidine groups (1.0 Gy) at the doses of 33.3, 100, and 300 mg/kg, respectively. The mice were irradiated to absorbed dose of 1.0 Gy with ,60,Co γ-rays at 8.33 mGy/min. Cimetidine was administered intragastrically 6 d before irradiation and 5 h after irradiation separately. Twenty-four hours after irradiation, the apoptosis rate of spleen lymphocytes, T lymphocyte subsets, and B lymphocytes in spleen, the content of IL-2, IL-4, IL-6, IL-12, IFN-γ, and TNF-α in serum were detected. Compared with normal control group, the apoptosis rate of spleen lymphocytes increased significantly (,p,<, 0.01). The percentage of CD3,+, CD3,+,CD8,+, T lymphocytes, CD3,−,CD19,+, B lymphocytes, and the content of IL-4, IL-12, and TNF-α in serum decreased significantly (,p,<, 0.01,p,<, 0.05). Compared with model control group, the apoptosis rate of spleen lymphocytes decreased (,p,<, 0.01), while the content of IL-4, IL-6, IL-12, IFN-γ, and TNF-α increased in serum of cimetidine groups (,p,<, 0.01,p,<, 0.05). The percentage of CD3,+, CD3,+,CD4,+, CD3,+,CD8,+, and CD3,−,CD19,+, cells increased in 300 mg/kg cimetidine group (,p,<, 0.01,p,<, 0.05). Cimetidine could improve the immune functions of mice irradiated by γ-rays to 1.0 Gy.
关键词
西咪替丁低剂量率电离辐射免疫功能细胞凋亡
Keywords
CimetidineLow-dose-rate radiationImmune functionApoptosis
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