酵母β-葡聚糖对人脐静脉内皮细胞的辐射防护作用

刘芳; 王转子; 李文建; 魏巍; 党秉荣

doi:10.11889/j.1000-3436.2018.rrj.36.040201

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酵母β-葡聚糖对人脐静脉内皮细胞的辐射防护作用

辐射生物学与医学 | 更新时间：2021-12-10

- 酵母β-葡聚糖对人脐静脉内皮细胞的辐射防护作用
- Radioprotective effect of beta-D-glucan derived from Saccharomyces cerevisiae on human umbilical vein endothelial cells
- 辐射研究与辐射工艺学报 2018年36卷第4期
- 作者机构：
  
  1.中国科学院近代物理研究所兰州 730000
  2.中国科学院大学北京 100049
- 作者简介：
  
  [ "刘芳, 女, 出生于1992年7月, 2014年于兰州大学获学士学位, 现为中国科学院近代物理研究所在读博士研究生, 专业为生物物理学, E-mail:liuf@impcas.ac.cn", "LIU Fang (Female) was born in July 1992, and received her bachelor's degree from Lanzhou University in 2014. Now she is a doctoral candidate at Institute of Modern Physics, Chinese Academy of Sciences, major in biophysics.E-mail:liuf@impcas.ac.cn" ]
  王转子, 博士, 副研究员, E-mail:wangzz@impcas.ac.cn Ph.D. WANG Zhuanzi, associate professor, E-mail:wangzz@impcas.ac.cn
- 基金信息：
  
  国家自然科学基金项目(11575259)
- DOI：10.11889/j.1000-3436.2018.rrj.36.040201
  中图分类号：
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刘芳, 王转子, 李文建, 等. 酵母β-葡聚糖对人脐静脉内皮细胞的辐射防护作用[J]. 辐射研究与辐射工艺学报, 2018,36(4):040201-7.

Fang LIU, Zhuanzi WANG, Wenjian LI, et al. Radioprotective effect of beta-D-glucan derived from Saccharomyces cerevisiae on human umbilical vein endothelial cells[J]. Journal of Radiation Research and Radiation Processing, 2018,36(4):040201-7.
刘芳, 王转子, 李文建, 等. 酵母β-葡聚糖对人脐静脉内皮细胞的辐射防护作用[J]. 辐射研究与辐射工艺学报, 2018,36(4):040201-7. DOI： 10.11889/j.1000-3436.2018.rrj.36.040201.

Fang LIU, Zhuanzi WANG, Wenjian LI, et al. Radioprotective effect of beta-D-glucan derived from Saccharomyces cerevisiae on human umbilical vein endothelial cells[J]. Journal of Radiation Research and Radiation Processing, 2018,36(4):040201-7. DOI： 10.11889/j.1000-3436.2018.rrj.36.040201.

摘要

研究酵母β-葡聚糖(BG)对X射线辐照致人脐静脉内皮细胞(Eahy926)损伤的防护作用。用四甲基偶氮唑盐(MTT)法评价BG的Eahy926细胞毒性，筛选出BG的最佳浓度和作用时间(10 μg/mL, 24 h)。用克隆存活法分析BG对Eahy926细胞辐射敏感性的影响。在X射线辐照至吸收剂量2 Gy后0.5和24 h，用流式细胞仪分析细胞凋亡分布、DNA损伤和细胞内活性氧(ROS)水平，并用试剂盒检测超氧化物歧化酶(SOD)活性和过氧化氢酶(CAT)活性。结果显示：BG对Eahy926细胞无毒性作用；与单独辐照组相比，BG预处理能减轻X射线辐射损伤，显著提高X射线辐照后细胞的克隆存活率；辐照后同一时间点，BG预处理组细胞DNA损伤、细胞凋亡率和细胞内ROS水平都明显低于单独辐照组；而BG预处理组细胞SOD和CAT酶活性均明显高于单独辐照组；在辐照后0.5和24 h，BG预处理组DNA损伤修复率高于单独辐照组。结果表明，BG对X射线造成的Eahy926细胞损伤有一定防护作用，其机制可能与BG清除细胞内自由基、提高抗氧化酶活性、减轻DNA损伤和细胞凋亡、促进DNA损伤修复有关。

Abstract

To explore the radioprotective effect of ,Saccharomyces cerevisiae,-derived-beta-D-glucan (BG) on human umbilical vein endothelial cells (Eahy926), cytotoxicity of BG on Eahy926 cells was detected by 3-(4, 5- Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), and the optimum concentration and pretreatment time were confirmed (10 μg/mL, 24 h). The clone formation assay was used to detect the BG effect on radiosensitivity of Eahy926 cells. At 0.5 h and 24 h after X-ray irradiation at a absorbed dose of 2 Gy, the apoptosis, DNA damage, and intracellular reactive oxygen species (ROS) of Eahy926 cells in each group were measured through flow cytometry, and the enzyme activities of superoxide dismutase (SOD) and catalase (CAT) were also detected. The results showed that BG was non-toxic to Eahy926 cells. BG pretreatment could decrease the cell damage caused by X-rays and significantly increase clone survival rates. At 0.5 h and 24 h after irradiation, the DNA damage, cell apoptosis rates, and intracellular ROS level of the BG pretreatment group were significantly lower than those of the 2 Gy group, and the enzyme activities of SOD and CAT were higher than those of the 2 Gy group. At 0.5 h and 24 h after irradiation, the repair rates of DNA damage in the BG pretreatment group were higher than those in the 2 Gy group. The results indicate that BG has a radioprotection effect on Eahy926 cells, and the mechanism may be attributed to BG's effect on scavenge of ROS, enhancement of antioxidase activities, reduction of DNA damage and cell apoptosis, and promotion of the repair of DNA damage.

关键词

酵母β-葡聚糖X射线辐射人脐静脉内皮细胞(Eahy926)辐射防护

Keywords

Saccharomyces cerevisiae-derived-beta-D-glucanX-ray irradiationHuman umbilical vein endothelial cell (Eahy926)Radioprotection

references

D G Kirsch , P M Santiago , E D Tomasso , 等 . p53 controls radiation-induced gastrointestinal syndrome in mice independent of apoptosis . Science , 2010 . 327 (5965 ):593 -596 . DOI:10.1126/science.1166202http://doi.org/10.1126/science.1166202 .

B Baselet , N Belmans , E Coninx , 等 . Functional gene analysis reveals cell cycle changes and inflammation in endothelial cells irradiated with a single X-ray dose . Frontiers in Pharmacology , 2017 . 8 (213 ):1 -13 . DOI:10.3389/fphar.2017.00213http://doi.org/10.3389/fphar.2017.00213 .

M Hofer , M Pospíšil . Modulation of animal and human hematopoiesis by β-glucans: a review . Molecules , 2011 . 16 (9 ):7969 -7979 . DOI:10.3390/molecules16097969http://doi.org/10.3390/molecules16097969 .

E A Murphy , J M Davis , M D Carmichael . Immune modulating effects of β-glucan . Current Opinion in Clinical Nutrition & Metabolic Care , 2010 . 13 (6 ):656 -661 . DOI:10.1097/mco.0b013e32833f1afbhttp://doi.org/10.1097/mco.0b013e32833f1afb .

B Du , C Y Lin , Z X Bian , 等 . An insight into anti-inflammatory effects of fungal beta-glucans . Trends in Food Science & Technology , 2015 . 41 (1 ):49 -59 . DOI:10.1016/j.tifs.2014.09.002http://doi.org/10.1016/j.tifs.2014.09.002 .

M Hofer , Z Hoferová , M Falk . Pharmacological modulation of radiation damage. Does it exist a chance for other substances than hematopoietic growth factors and cytokines? . International Journal of Molecular Sciences , 2017 . 18 (7 ):1385 -1404 . DOI:10.3390/ijms18071385http://doi.org/10.3390/ijms18071385 .

W Zhao , X J Jian , W W Deng , 等 . Antioxidant activities of Ganoderma lucidum polysaccharides and their role on DNA damage in mice induced by cobalt-60 gamma-irradiation . Food & Chemical Toxicology , 2012 . 50 (2 ):303 -309 . DOI:10.1016/j.fct.2011.10.071http://doi.org/10.1016/j.fct.2011.10.071 .

马斌博 , 王转子 , 魏巍 , 等 . 羧甲基-β-1, 3-葡聚糖对人B淋巴母细胞的辐射防护作用 . 辐射研究与辐射工艺学报 , 2016 . 34 (2 ):020205 DOI:10.11889/j.1000-3436.2016.rrj.34.020205http://doi.org/10.11889/j.1000-3436.2016.rrj.34.020205 http://www.j.sinap.ac.cn/fushe/CN/abstract/abstract302.shtmlhttp://www.j.sinap.ac.cn/fushe/CN/abstract/abstract302.shtml, .

Binbo MA , Zhuanzi WANG , Wei WEI , 等 . Protection effects of carboxymethyl-β-1, 3-glucan on human B lymphoblasts' injury induced by radiation . Journal of Radiation Research and Radiation Processing , 2016 . 34 (2 ):020205 DOI:10.11889/j.1000-3436.2016.rrj.34.020205http://doi.org/10.11889/j.1000-3436.2016.rrj.34.020205 http://www.j.sinap.ac.cn/fushe/CN/abstract/abstract302.shtmlhttp://www.j.sinap.ac.cn/fushe/CN/abstract/abstract302.shtml, .

Y H Gu , Y Takagi , T Nakamura , 等 . Enhancement of radioprotection and anti-tumor immunity by yeast-derived beta-glucan in mice . Journal of Medicinal Food , 2005 . 8 (2 ):154 -158 . DOI:10.1089/jmf.2005.8.154http://doi.org/10.1089/jmf.2005.8.154 .

L Hong , E D Stanchina , X K Zhou , 等 . Maitake beta-glucan enhances unbilical cord blood stem cell transplantation in the NOD/SCID mouse . Experimental Biology and Medicine , 2009 . 234 (3 ):342 -353 . DOI:10.3181/0807-rm-226http://doi.org/10.3181/0807-rm-226 .

K T Baldvin , K S Carbajal , B M Segal , 等 . Neuroinflammation triggered by beta-glucan/dectin-1 signaling enables CNS axon regeneration . Proceedings of the National Academy of Sciences of the United States of America , 2015 . 112 (8 ):2581 -2586 . DOI:10.1073/pnas.1423221112http://doi.org/10.1073/pnas.1423221112 .

Z Mei , J A Kim . Effect of molecular size and modification pattern on the internalization of water soluble β-(1→3)- (1→4)-glucan by primary murine macrophages . International Journal of Biochemistry & Cell Biology , 2012 . 44 (6 ):914 -927 . DOI:10.1016/j.biocel.2012.02.018http://doi.org/10.1016/j.biocel.2012.02.018 .

司徒镇强 , 吴军正 . 细胞培养 , :北京世界图书出版公司 , 2003 . 186 -187. .

Zhenqiang SITU , Junzheng WU . Cell culture , :Beijing World Publishing Corporation , 2003 . 186 -187. .

G Demir , H O Klein , N Mandel-molinas , 等 . Beta glucan induces proliferation and activation of monocytes in peripheral blood of patients with advanced breast cancer . International Immunopharmacology , 2007 . 7 (1 ):113 -116 . DOI:10.1016/j.intimp.2006.08.011http://doi.org/10.1016/j.intimp.2006.08.011 .

P L Olive , J P Banáth , M Keyes . Residual gammaH2AX after irradiation of human lymphocytes and monocytes in vitro and its relation to late effects after prostate brachytherapy . Radiotherapy & Oncology , 2008 . 86 (3 ):336 -346 . DOI:10.1016/j.radonc.2007.09.002http://doi.org/10.1016/j.radonc.2007.09.002 .

R J Oliveira , R Matuo , A F D Silva , 等 . Protective effect of beta-glucan extracted from Saccharomyces cerevisiae, against DNA damage and cytotoxicity in wild-type (k1) and repair-deficient (xrs5) CHO cells . Toxicology in Vitro , 2007 . 21 (1 ):41 -52 . DOI:10.1016/j.tiv.2006.07.018http://doi.org/10.1016/j.tiv.2006.07.018 .

B Halliwell . The wanderings of a free radical . Free Radical Biology & Medicine , 2009 . 46 (5 ):531 -542 . DOI:10.1016/j.freeradbiomed.2008.11.008http://doi.org/10.1016/j.freeradbiomed.2008.11.008 .

E I Azzam , J P Jay-gerin , D Pain . Ionizing radiation- induced metabolic oxidative stress and prolonged cell injury . Cancer Letters , 2012 . 327 (1-2 ):48 -60 . DOI:10.1016/j.canlet.2011.12.012http://doi.org/10.1016/j.canlet.2011.12.012 .

G Kogan , A Stasko , K Bauerova , 等 . Antioxidant properties of yeast (1→3)-β-D-glucan studied by electron paramagnetic resonance spectroscopy and its activity in the adjuvant arthritis . Carbohydrate Polymers , 2005 . 61 (1 ):18 -28 . DOI:10.1016/j.carbpol.2005.02.010http://doi.org/10.1016/j.carbpol.2005.02.010 .

E Tsiapali , S Whaley , J Kalbfleisch , 等 . Glucans exhibit weak antioxidant activity, but stimulate macrophage free radical activity . Free Radical Biology & Medicine , 2001 . 30 (4 ):393 -402 . DOI:10.1016/s0891-5849(00)00485-8http://doi.org/10.1016/s0891-5849(00)00485-8 .

J Kasapović , V Stojiljković , L Gavrilović , 等 . Antioxidant protection against curative and palliative doses of ionizing irradiation in human blood decreases with aging . Scientific World Journal , 2012 . 2012 (3 ):1 -8 . DOI:10.1100/2012/982594http://doi.org/10.1100/2012/982594 .

M Adzić , A Nićiforović , B Zarić , 等 . Cell culture conditions potentiate differences in the response to ionizing radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects . Redox Report , 2008 . 13 (1 ):17 -22 . DOI:10.1179/135100008X259088http://doi.org/10.1179/135100008X259088 .

D M Reid , N A R Gow , G D Brown . Pattern recognition: recent insights from Dectin-1 . Current Opinion in Immunology , 2009 . 21 (1 ):30 -37 . DOI:10.1016/j.coi.2009.01.003http://doi.org/10.1016/j.coi.2009.01.003 .

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