类神经细胞释放多巴胺的实时监测

李敏; 张月月; 刘江波; 王丽华; 李茜; 左小磊

doi:10.11889/j.1000-3436.2018.rrj.36.050201

您当前的位置：

首页 >

文章列表页 >

类神经细胞释放多巴胺的实时监测

辐射生物学与医学 | 更新时间：2021-12-10

- 类神经细胞释放多巴胺的实时监测
- Monitoring dopamine release from neuron-like cells
- 辐射研究与辐射工艺学报 2018年36卷第5期
- 作者机构：
  
  1.中国科学院上海应用物理研究所物理生物学研究室上海光源生物成像中心上海 201800
  2.中国科学院大学北京 100049
  3.上海交通大学医学院附属仁济医院上海 200127
- 作者简介：
  
  [ "李敏, 女, 1990年5月出生, 2018年6月于中国科学院上海应用物理研究所获得博士学位, 从事电化学生物传感研究, E-mail: mlisinap@163.com", "LI Min (female) was born in May 1990 and received her doctor's degree in Shanghai Institute of Applied Physics, Chinese Academy of Sciences in June 2018, majoring in electrochemical biosensing. E-mail: mlisinap@163.com" ]
  李茜, 副研究员, E-mail: liqian@sinap.ac.cn LI Qian, associate professor, E-mail: E-mail: liqian@sinap.ac.cn
  左小磊, 研究员, E-mail: zuoxiaolei@sjtu.edu.cn ZUO Xiaolei, professor, E-mail: zuoxiaolei@sjtu.edu.cn
- 基金信息：
  
  国家自然科学基金项目(21422508);国家自然科学基金项目(31470960);中国科学院科研装备研制项目(yz201438)
- DOI：10.11889/j.1000-3436.2018.rrj.36.050201
  中图分类号：
扫描看全文
李敏, 张月月, 刘江波, 等. 类神经细胞释放多巴胺的实时监测[J]. 辐射研究与辐射工艺学报, 2018,36(5):050201-6.

Min LI, Yueyue ZHANG, Jiangbo LIU, et al. Monitoring dopamine release from neuron-like cells[J]. Journal of Radiation Research and Radiation Processing, 2018,36(5):050201-6.
李敏, 张月月, 刘江波, 等. 类神经细胞释放多巴胺的实时监测[J]. 辐射研究与辐射工艺学报, 2018,36(5):050201-6. DOI： 10.11889/j.1000-3436.2018.rrj.36.050201.

Min LI, Yueyue ZHANG, Jiangbo LIU, et al. Monitoring dopamine release from neuron-like cells[J]. Journal of Radiation Research and Radiation Processing, 2018,36(5):050201-6. DOI： 10.11889/j.1000-3436.2018.rrj.36.050201.

摘要

采用火焰刻蚀法制备碳纤维纳米电极（CFNE），通过扫描电子显微镜对纳米电极直径进行表征，采用循环伏安法对纳米电极的电化学性能进行验证。选用差分脉冲伏安法验证了纳米电极对多巴胺（Dopamine，DA）的体外响应，最后采用膜片钳电压钳模式，施加电压700 mV（Ag/AgCl为参比），对高分化的PC12细胞施加高钾刺激，实时监测DA的释放。结果表明：CFNE的直径可低至~100 nm，电极表面存在优良的非线性扩散特性；CFNE在体外对DA有灵敏的响应，检测限达10 pmol/L；对PC12细胞高钾刺激后，实时监测到PC12细胞单个释放位点的DA释放过程。

Abstract

A carbon fiber nanoelectrode (CFNE) was prepared using the flame-etching method. Scanning electron microscopy and cyclic voltammetry were used to characterize its diameter and the electrochemical properties, respectively. Differential pulse voltammetry was used to detect dopamine (DA) ,in vitro,. A patch clamp in the voltage-clamp mode was used to monitor the release of DA from PC12 cells after stimulation with an elevated K,+, solution, maintaining the voltage at 700 mV. The results showed that CFNE was successfully prepared with a diameter of ~100 nm, and excellent nonlinear diffusion behavior was observed on the nanoelectrode surface. Further, the nanoelectrode showed excellent response to DA with the limit of detection of 10 pmol/L. After PC12 cells were stimulated with the elevated K,+, solution, the DA release process of PC12 cells at a single release site was monitored in real time.

关键词

碳纤维纳米电极类神经细胞单释放位点多巴胺实时监测

Keywords

Carbon fiber nanoelectrodeNeuron-like cellSingle release siteDopamineReal-time monitoring

references

P Damier , E C Hirsch , Y Agid , 等 . The substantia nigra of the human brain-Ⅱ. Patterns of loss of dopamine-containing neurons in Parkinson's disease . Brain , 1999 . 122 (8 ):1437 -1448 . DOI:10.1093/brain/122.8.1437http://doi.org/10.1093/brain/122.8.1437 .

刘林林 , 田梅 , 傅海清 , 等 . 电离辐射对小鼠胸腺中多巴胺羟化酶及β2受体mRNA表达的影响 . 吉林大学学报(医学版) , 2003 . 29 (2 ):128 -130 . http://d.old.wanfangdata.com.cn/Periodical/bqeykdxxb200302002http://d.old.wanfangdata.com.cn/Periodical/bqeykdxxb200302002, .

Linlin LIU , Mei TIAN , Haiqing FU , 等 . Effect of ionizing radiation on DBH mRNA and β2-AR mRNA expressions in thymus in mice . Journal of Jilin University (Medicine Edition) , 2003 . 29 (2 ):128 -130 . http://d.old.wanfangdata.com.cn/Periodical/bqeykdxxb200302002http://d.old.wanfangdata.com.cn/Periodical/bqeykdxxb200302002, .

刘林林 , 尉军 , 孙宝胜 , 等 . 低剂量辐射对多巴胺β羟化酶活性及其基因表达的影响 . 中华放射医学与防护杂志 , 2002 . 22 (5 ):343 -345 . DOI:10.3760/cma.j.issn.0254-5098.2002.05.011http://doi.org/10.3760/cma.j.issn.0254-5098.2002.05.011.

Linlin LIU , Jun WEI , Baosheng SUN , 等 . Effect of low dose radiation on β-DBH activity and gene expression . Chinese Journal of Radiological Medicine and Protection , 2002 . 22 (5 ):343 -345 . DOI:10.3760/cma.j.issn.0254-5098.2002.05.011http://doi.org/10.3760/cma.j.issn.0254-5098.2002.05.011.

R B Zhong , Q Tang , S P Wang , 等 . Self-assembly of enzyme-like nanofibrous G-molecular hydrogel for printed flexible electrochemical sensors . Advanced Materials , 2018 . 30 (12 ):1706887(1-8) DOI:10.1002/adma.201706887http://doi.org/10.1002/adma.201706887 .

Z L Ge , H Pei , L H Wang , 等 . Electrochemical single nucleotide polymorphisms genotyping on surface immobilized three-dimensional branched DNA nanostructure . Science China Chemistry , 2011 . 54 (8 ):1273 -1276 . DOI:10.1007/s11426-011-4327-6http://doi.org/10.1007/s11426-011-4327-6 .

X M Qu , D Zhu , G B Yao , 等 . An exonuclease Ⅲ-powered, on-particle stochastic DNA walker . Angewandte Chemie International Edition , 2017 . 56 (7 ):1855 -1858 . DOI:10.1002/anie.201611777http://doi.org/10.1002/anie.201611777 .

Q S Chen , H J Liu , L Wonsuk , 等 . Self-assembled DNA tetrahedral optofluidic lasers with precise and tunable gain control . Lab Chip , 2013 . 13 (17 ):3351 -3354 . DOI:10.1039/c3lc50629khttp://doi.org/10.1039/c3lc50629k .

G B Yao , H Pei , J Li , 等 . Clicking DNA to gold nanoparticles:poly-adenine-mediated formation of monovalent DNA-gold nanoparticle conjugates with nearly quantitative yield . NPG Asia Mater , 2015 . 7 e159 DOI:10.1038/am.2014.131http://doi.org/10.1038/am.2014.131 .

D Zhu , P Song , J W Shen , 等 . PolyA-mediated DNA assembly on gold nanoparticles for thermodynamically favorable and rapid hybridization analysis . Analytical Chemistry , 2016 . 88 (9 ):4949 -4954 . DOI:10.1021/acs.analchem.6b00891http://doi.org/10.1021/acs.analchem.6b00891 .

X F Yang , J Li , H Pei , 等 . Pattern recognition analysis of proteins using DNA decorated catalytic gold nanoparticles . Small , 2013 . 9 (17 ):2844 -2849 . DOI:10.1002/smll.201202772http://doi.org/10.1002/smll.201202772 .

L Z Chen , J Chao , X M Qu , 等 . Probing cellular molecules with polyA-based engineered aptamer nanobeacon . ACS Applied Materials & Interfaces , 2017 . 9 (9 ):8014 -8020 . DOI:10.1021/acsami.6b16764http://doi.org/10.1021/acsami.6b16764 .

X M Qu , H B Zhao , H Chen , 等 . Convection-driven pull-down assays in nanoliter droplets using scaffolded aptamers . Analytical Chemistry , 2017 . 89 (6 ):3468 -3473 . DOI:10.1021/acs.analchem.6b04475http://doi.org/10.1021/acs.analchem.6b04475 .

R N Adams . Probing brain chemistry with electroanalytical techniques . Analytical Chemistry , 1976 . 48 (14 ):1126A -1138A . DOI:10.1021/ac50008a001http://doi.org/10.1021/ac50008a001 .

T L Colliver , E J Hess , E N Pothos , 等 . Quantitative and statistical analysis of the shape of amperometric spikes recorded from two populations of cells . Journal of Neurochemistry , 2000 . 74 (3 ):1086 -1097 . DOI:10.1046/j.1471-4159.2000.741086.xhttp://doi.org/10.1046/j.1471-4159.2000.741086.x .

L A Sombers , M M Maxson , A G Ewing . Loaded dopamine is preferentially stored in the halo portion of PC12 cell dense core vesicles . Journal of Neurochemistry , 2005 . 93 (5 ):1122 -1131 . DOI:10.1111/j.1471-4159.2005.03087.xhttp://doi.org/10.1111/j.1471-4159.2005.03087.x .

张月月 , 左小磊 , 樊春海 . 高时空分辨率的神经递质电化学传感检测技术发展与展望 . 中国科学院院刊 , 2017 . 32 (12 ):1290 -1302 . DOI:10.16418/j.issn.1000-3045.2017.12.003http://doi.org/10.16418/j.issn.1000-3045.2017.12.003 .

Yueyue ZHANG , Xiaolei ZUO , Chunhai FAN . Electrochemical sensing of neurotransmitters with high temporal and spatial resolution . Bulletin of Chinese Academy of Sciences , 2017 . 32 (12 ):1290 -1302 . DOI:10.16418/j.issn.1000-3045.2017.12.003http://doi.org/10.16418/j.issn.1000-3045.2017.12.003 .

C Amatore , S Arbault , Y Bouret , 等 . Invariance of exocytotic events detected by amperometry as a function of the carbon fiber microelectrode diameter . Analytical Chemistry , 2009 . 81 (8 ):3087 -3093 . DOI:10.1021/ac900059shttp://doi.org/10.1021/ac900059s .

R M Wightman . Probing cellular chemistry in biological systems with microelectrodes . Science , 2006 . 311 (5767 ):1570 -1574 . DOI:10.1126/science.1120027http://doi.org/10.1126/science.1120027 .

T L Colliver , E J Hess , A G Ewing . Amperometric analysis of exocytosis at chromaffin cells from genetically distinct mice . Journal of Neurochemistry , 2001 . 105 (1 ):95 -103 . DOI:10.1016/S0165-0270(00)00359-9http://doi.org/10.1016/S0165-0270(00)00359-9 .

I M Robinson , J M Finnegan , J R Monck , 等 . Colocalization of calcium entry and exocytotic release sites in adrenal chromaffin cells . Proceedings of the National Academy of Sciences of the United States of America , 1995 . 92 (7 ):2474 DOI:10.1073/pnas.92.7.2474http://doi.org/10.1073/pnas.92.7.2474 .

R M Wightman , J A Jankowski , R T Kennedy , 等 . Temporally resolved catecholamine spikes correspond to single vesicle release from individual chromaffin cells . Proceedings of the National Academy of Sciences of the United States of America , 1991 . 88 (23 ):10754 -10758 . DOI:10.1073/pnas.88.23.10754http://doi.org/10.1073/pnas.88.23.10754 .

S E Zerby , A G Ewing . Electrochemical monitoring of individual exocytotic events from the varicosities of differentiated PC12 cells . Brain Research , 1996 . 712 (1 ):1 -10 . DOI:10.1016/0006-8993(95)01383-0http://doi.org/10.1016/0006-8993(95)01383-0 .

K D Kozminski , D A Gutman , V Davila , 等 . Voltammetric and pharmacological characterization of dopamine release from single exocytotic events at rat pheochromocytoma (PC12) cells . Analytical Chemistry , 1998 . 70 (15 ):3123 -3130 . DOI:10.1021/ac980129fhttp://doi.org/10.1021/ac980129f .

朱丹 , 左小磊 , 樊春海 . 纳米花状超微金电极的构筑及其电化学生物传感应用 . 中国科学:化学 , 2015 . 45 (11 ):1214 -1219 . http://cdmd.cnki.com.cn/Article/CDMD-80014-1015033061.htmhttp://cdmd.cnki.com.cn/Article/CDMD-80014-1015033061.htm, .

Dan ZHU , Xiaolei ZUO , Chunhai FAN . Fabrication of nanometer-sized gold flower microelectrodes for electrochemical biosensing applications . Science China Chemistry , 2015 . 45 (11 ):1214 -1219 . http://cdmd.cnki.com.cn/Article/CDMD-80014-1015033061.htmhttp://cdmd.cnki.com.cn/Article/CDMD-80014-1015033061.htm, .

T K Chen , G Luo , A G Ewing . Amperometric monitoring of stimulated catecholamine release from rat pheochromocytoma (PC12) cells at the zeptomole level . Analytical Chemistry , 1994 . 66 (19 ):3031 -3035 . DOI:10.1021/ac00091a007http://doi.org/10.1021/ac00091a007 .

B A Brian , G Y Chen , A G David , 等 . Demonstration of two distributions of vesicle radius in the dopamine neuron of planorbis corneus from electrochemical data . Journal of Neuroscience Methods , 1999 . 88 (2 ):153 -161 . DOI:10.1016/S0165-0270(99)00024-2http://doi.org/10.1016/S0165-0270(99)00024-2 .

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

暂无数据