伽马射线辐照对小鼠认知功能及神经元的影响

乃爱桃; 王贞; 唐丽云; 万炜; 刘政海; 徐杨; 艾小红; 何淑雅; 曹文宇

doi:10.11889/j.1000-3436.2018.rrj.36.050202

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伽马射线辐照对小鼠认知功能及神经元的影响

辐射生物学与医学 | 更新时间：2021-12-10

- 伽马射线辐照对小鼠认知功能及神经元的影响
- Effects of gamma-ray irradiation on cognitive function and neuronal death in mice
- 辐射研究与辐射工艺学报 2018年36卷第5期
- 作者机构：
  
  1.南华大学附属第一医院肿瘤放疗科衡阳 421001
  2.南华大学医学院应用解剖与生殖医学研究所衡阳 421001
  3.南华大学医学院生理教研室衡阳 421001
  4.南华大学公共卫生学院放射医学教研室衡阳 421001
- 作者简介：
  
  [ "乃爱桃, 男, 1992年7月出生, 2015年毕业于南华大学, 现为南华大学附属第一医院肿瘤放射治疗学专业硕士研究生, E-mail: 499108712@qq.com", "NAI Aitao (male) was born in July 1992, and graduated from University of South China in 2015. Now he is a master candidate in the Department of Radiation Oncology, The First Affiliated Hospital of University of South China. E-mail: 499108712@qq.com" ]
  何淑雅, 博士, 教授, E-mail: heshuya8502@163.com Ph.D. HE Shuya, professor, E-mail: heshuya8502@163.com
  曹文宇, 博士, 讲师, E-mail: marksman0@163.com Ph.D. CAO Wenyu, lecturer, E-mail: marksman0@163.com
- 基金信息：
  
  湖南省自然科学基金青年基金项目(2018JJ3460);湖南省自然科学基金省市联合基金项目(2018JJ4034);湖南省教育厅重点项目(14A125);湖南省教育厅优秀青年项目(17B230);南华大学博士科研启动基金(2015XQD27);南华大学博士科研启动基金(2015XQD28);2018年湖南省研究生科研创新项目资助
- DOI：10.11889/j.1000-3436.2018.rrj.36.050202
  中图分类号：
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乃爱桃, 王贞, 唐丽云, 等. 伽马射线辐照对小鼠认知功能及神经元的影响[J]. 辐射研究与辐射工艺学报, 2018,36(5):050202-6.

Aitao NAI, Zhen WANG, Liyun TANG, et al. Effects of gamma-ray irradiation on cognitive function and neuronal death in mice[J]. Journal of Radiation Research and Radiation Processing, 2018,36(5):050202-6.
乃爱桃, 王贞, 唐丽云, 等. 伽马射线辐照对小鼠认知功能及神经元的影响[J]. 辐射研究与辐射工艺学报, 2018,36(5):050202-6. DOI： 10.11889/j.1000-3436.2018.rrj.36.050202.

Aitao NAI, Zhen WANG, Liyun TANG, et al. Effects of gamma-ray irradiation on cognitive function and neuronal death in mice[J]. Journal of Radiation Research and Radiation Processing, 2018,36(5):050202-6. DOI： 10.11889/j.1000-3436.2018.rrj.36.050202.

摘要

57只2月龄雌性昆明小鼠分别接受不同剂量,137,Cs γ射线（0、4、6、8、10 Gy）单次全身照射，观察小鼠生存率及体重变化情况，35 d后进行旷场实验、新旧位置识别实验行为学检测，并采用Western blot方法检测小鼠海马区神经元标记物NeuN的表达。结果表明：4 Gy剂量组小鼠生存率100%，且一般健康状况较好，而给予8 Gy及10 Gy吸收剂量时，小鼠死亡率明显增加；与对照组相比，4 Gy及6 Gy剂量组小鼠在新旧位置识别实验中，对新位置的时间辨别指数百分比均明显降低（36.04%±4.39% ,vs,. 11.45%±6.34% ,vs,. -1.40%±9.27%，,p,<, 0.05）；海马区NeuN蛋白表达均明显下调（1.000 ±0.045 ,vs,. 0.795 ±0.052 ,vs,. 0.332 ±0.024，,p,<, 0.05），且6 Gy剂量组下调趋势更加明显。研究表明，在一定剂量范围内（6 Gy及以下吸收剂量），,137,Cs γ射线全身照射诱导小鼠认知功能障碍及神经元损害随着吸收剂量的增加而越发明显。

Abstract

Fifty-seven female Kunming mice aged 2 months received full-body irradiation once with ,137,Cs γ-rays at doses of 0, 4, 6, 8, and 10 Gy. The survival rate and body weight were then observed for 35 d. The animals' behaviors were analyzed using open-field and novelty location tests 35 d post irradiation. The expression of the neuronal marker NeuN in hippocampus was determined using western blot. The results showed that the mice in the 4 Gy group exhibited a higher survival rate and superior general status than did those in the other groups. Compared with the control group, the mice in the 4 and 6 Gy groups had a lower discrimination index in the novelty location test (36.04%±4.39% ,vs,. 11.45%±6.34% ,vs,. -1.40%±9.27%, respectively,p,<, 0.05), and had a remarkably lower level of NeuN expression in hippocampus (1.000±0.045 ,vs,. 0.795±0.052 ,vs,. 0.332±0.024, respectively,p,<, 0.05). Meanwhile, the mice in the 6 Gy group exhibited more serious cognitive dysfunction and neuronal death than did those in the 4 Gy group. The mice in the 8 and 10 Gy groups showed a higher death rate than those in the 4 and 6 Gy groups. However, no significant difference in survival rate was found between the mice in the 4 and 6 Gy groups. Our results suggest that ,137,Cs γ-ray irradiation can induce cognitive dysfunction and neuronal death in a dose-dependent manner at a dose of ,<, 6 Gy.

关键词

电离辐射γ射线认知功能神经元

Keywords

Ionizing radiationγ-raysCognitive functionNeuron

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