一种辐射防护中药组方对小鼠脾脏辐射损伤的保护作用

周茜; 唐瑛; 孙欢; 陈雪娇

doi:10.11889/j.1000-3436.2022-0048

您当前的位置：

首页 >

文章列表页 >

一种辐射防护中药组方对小鼠脾脏辐射损伤的保护作用

辐射生物学与医学 | 更新时间：2023-04-20

- 一种辐射防护中药组方对小鼠脾脏辐射损伤的保护作用
- Protective effects of a Chinese medicinal formula on the spleen of irradiated mice
- 辐射研究与辐射工艺学报 2023年41卷第2期页码：20302
- 作者机构：
  
  1.湖北文理学院襄阳 441053
  2.中国人民解放军海军特色医学中心上海 200433
  3.攀枝花市卫生健康委员会攀枝花 617000
- 作者简介：
  
  周茜，女，1987年2月出生，2013年于湖北中医药大学药理学专业获硕士学位，现就职于湖北文理学院
  唐瑛，研究员，E-mail: 1446329418@qq.com
- 基金信息：
  
  湖北省自然科学基金项目(2020CFB338);襄阳市科技局医疗卫生领域科技计划项目(2020ZD06)
- DOI：10.11889/j.1000-3436.2022-0048
  中图分类号：
扫描看全文
周茜, 唐瑛, 孙欢, 等. 一种辐射防护中药组方对小鼠脾脏辐射损伤的保护作用[J]. 辐射研究与辐射工艺学报, 2023,41(2):020302.

ZHOU Xi, TANG Ying, SUN Huan, et al. Protective effects of a Chinese medicinal formula on the spleen of irradiated mice[J]. Journal of Radiation Research and Radiation Processing, 2023,41(2):020302.
周茜, 唐瑛, 孙欢, 等. 一种辐射防护中药组方对小鼠脾脏辐射损伤的保护作用[J]. 辐射研究与辐射工艺学报, 2023,41(2):020302. DOI： 10.11889/j.1000-3436.2022-0048.

ZHOU Xi, TANG Ying, SUN Huan, et al. Protective effects of a Chinese medicinal formula on the spleen of irradiated mice[J]. Journal of Radiation Research and Radiation Processing, 2023,41(2):020302. DOI： 10.11889/j.1000-3436.2022-0048.

摘要

探讨由黄芪、刺五加、山药、葡萄籽及酸枣仁提取物混合组成的一种辐射防护中药组方（简称防辐组方）对辐射损伤小鼠脾脏的影响及保护作用。实验小鼠随机分为5组：对照组、模型组、防辐组方低，中，高剂量组（500 mg/kg、1 000 mg/kg、2 000 mg/kg）。6.5 Gy ,60,Co γ射线一次性全身照射建立辐射损伤模型。观察各组小鼠脾脏系数、脾脏组织病理学形态、Caspase-3，Caspase-9及细胞色素C活性、凋亡相关蛋白Bax、Bcl-2及P53蛋白表达、脾脏T淋巴亚群CD4,+,、CD8,+,百分比、CD4,+,/CD8,+,比值及各组脾脏细胞DNA损伤程度等指标的变化情况。实验结果显示：与模型组比较，给药组小鼠的体重及脾脏系数均有所增高。其中，中剂量组和高剂量组增高最为显著（,p,<,0.01或,p,<,0.05）；给药组小鼠脾脏中的CD4,+,、CD8,+,百分比、CD4,+,/CD8,+,比值及Bcl-2的蛋白表达均上升显著，Caspase-3，Caspase-9，细胞色素C活性及Bax、P53的蛋白表达有显著性下降（,p,<,0.01或,p,<,0.05）；同时各给药组小鼠脾脏细胞的Tail DNA%、Tail Length及Olive尾距（OTM）均有显著性下降（,p,<,0.01或,p,<,0.05）。病理组织学观察发现，与模型组比较，给药组小鼠脾脏的红白髓区清晰，淋巴细胞坏死及中性粒细胞浸润均有明显下降。本研究提示，防辐组方对辐射损伤小鼠的脾脏具有一定的保护作用。推测其保护作用机制，可能与抑制细胞凋亡和减少DNA损伤有关。

Abstract

A Chinese medicinal formula was prepared by combining extracts from ,Astragalus, Siberian ginseng, wild Yam, grape seed, and wild Jujube and assessed for the protective effect of the Fangfu formula powder on the spleen of radiation-injured mice. The mice were irradiated with a one-time ,60,Co γ ray 6.5 Gy dose exposure. The experimental mice were randomized into five equal groups: the control, the model , and the low, middle, and high-dose group (500 mg/kg, 1 000 mg/kg, 2 000 mg/kg, respectively). Changes in the spleen index, pathomorphisms in the spleen tissues, Caspase-3, Caspase-9, cytochrome C, CD4,+, CD8,+, the apoptosis-related proteins Bax, Bcl-2, and P53 protein expression, and the cell DNA damage in each group were measured. Compared with the model group, there was an increase in the body weight and spleen index of the dosed mice, with the most significant (p,<,0.01 or p,<,0.05) increase occurring in the middle- and high-dose groups. Furthermore, the Fangfu formula significantly (p,<,0.01 or p,<,0.05) increased the CD4,+, CD8,+, CD4,+,/CD8,+, percentages, and Bcl-2 protein expression and reduced the level of Caspase-3, Caspase-9, cytochrome C, Tail DNA%, Tail length, olive tail moment (OTM), Bax, and P53 protein expression in spleen tissue when compared with the model group. The histopathologic examination showed that the red and white pulp area of the spleen of the dosed mice was clear, and both lymphocyte necrosis and neutrophil infiltration were decreased significantly in the dosed mice as compared to that in the model group. The data suggest that the Fangfu Formula has a protective effect on the spleen of irradiated mice. These findings might be related to inhibition of DNA damage and apoptosis.

关键词

防辐组方辐射损伤小鼠脾脏

Keywords

Fangfu formulasRadiation injuryMiceSpleen

references

Bray F, Ferlay J, Soerjomataram I, et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J].CA: A Cancer Journal for Clinicians, 2018, 68(6): 394-424. DOI: 10.3322/caac.21492http://dx.doi.org/10.3322/caac.21492.

付海英, 吴国忠. 草本组方对γ射线全身照射小鼠的保护作用[J]. 辐射研究与辐射工艺学报, 2022, 40(2): 020301. DOI: 10.11889/j.1000-3436.2021-0205http://dx.doi.org/10.11889/j.1000-3436.2021-0205.

FU Haiying, WU Guozhong. Protection against γ ray-induced damage using herbal formulas[J]. Journal of Radiation Research and Radiation Processing, 2022, 40(2): 020301. DOI: 10.11889/j.1000-3436.2021-0205http://dx.doi.org/10.11889/j.1000-3436.2021-0205.

Gilbert E S. Ionising radiation and cancer risks: what have we learned from epidemiology?[J]. International Journal of Radiation Biology, 2009, 85(6): 467-482. DOI:10.1080/09553000902883836http://dx.doi.org/10.1080/09553000902883836.

Dowlath M J H, Karuppannan S K, Sinha P, et al. Effects of radiation and role of plants in radioprotection: a critical review[J]. Science of the Total Environment, 2021, 779: 146431. DOI: 10.1016/j.scitotenv.2021.146431http://dx.doi.org/10.1016/j.scitotenv.2021.146431.

薛琦, 王曼, 刘丽波. 表没食子儿茶素没食子酸酯的辐射防护作用及其机制研究进展[J]. 辐射研究与辐射工艺学报, 2020, 38(5): 050101. DOI: 10.11889/j.1000-3436. 2020.rrj.38.050101http://dx.doi.org/10.11889/j.1000-3436.2020.rrj.38.050101.

XUE Qi, WANG Man, LIU Libo. Research progress on the radioprotective effect of epigallocatechin gallate and its mechanism[J]. Journal of Radiation Research and Radiation Processing,2020,38(5): 050101. DOI: 10. 11889/j.1000-3436.2020.rrj. 38.050101http://dx.doi.org/10.11889/j.1000-3436.2020.rrj.38.050101.

李盼飞, 卢曦, 何昶昊, 等. 电离辐射致肠道急性损伤的中医病机探讨[J]. 环球中医药, 2020, 13(4): 616-619. DOI: 10.3969/j.issn.1674-1749.2020.04.012http://dx.doi.org/10.3969/j.issn.1674-1749.2020.04.012.

LI Panfei, LU Xi, HE Changhao, et al. Discussion on TCM pathogenesis of acute intestinal injury caused by ionizing radiation[J]. Global Traditional Chinese Medicine, 2020, 13(4): 616-619. DOI: 10.3969/j.issn. 1674-1749.2020.04.012http://dx.doi.org/10.3969/j.issn.1674-1749.2020.04.012.

Checker R, Patwardhan R S, Jayakumar S, et al. Chemical and biological basis for development of novel radioprotective drugs for cancer therapy[J]. Free Radical Research, 2021, 55(8): 828-858. DOI: 10.1080/10715762.2021.1876854http://dx.doi.org/10.1080/10715762.2021.1876854.

Chen T, Wang R N, Jiang W J, et al. Protective effect of astragaloside IV against paraquat-induced lung injury in mice by suppressing rho signaling[J]. Inflammation, 2016, 39(1): 483-492. DOI: 10.1007/s10753-015-0272-4http://dx.doi.org/10.1007/s10753-015-0272-4.

Lu M L, Tang F T, Zhang J, et al. Astragaloside IV attenuates injury caused by myocardial ischemia/reperfusion in rats via regulation of toll-like receptor 4/nuclear factor-κB signaling pathway[J]. Phytotherapy Research: PTR, 2015, 29(4): 599-606. DOI: 10.1002/ptr.5297http://dx.doi.org/10.1002/ptr.5297.

李思佳. 刺五加中改善辐射小鼠脑损伤的功能成分研究[D]. 哈尔滨: 哈尔滨工业大学, 2019.

LI Sijia. Study on the functional components of acanthopanax sentionsus for improving brain danage in irradiated mouse[D]. Harbin: Harbin Institute of Technology, 2019.

徐俊杰, 王珺, 杨占群. 山药多糖对低强度连续微波辐射小鼠免疫系统功能的影响[J]. 上海中医药杂志, 2019, 53(11): 82-85. DOI: 10.16305/j.1007-1334.2019. 11.017http://dx.doi.org/10.16305/j.1007-1334.2019.11.017.

XU Junjie, WANG Jun, YANG Zhanqun. Effect of yam polysaccharides on immune system function of mice exposed to continuous low-density microwave radiation[J]. Shanghai Journal of Traditional Chinese Medicine, 2019, 53(11): 82-85. DOI: 10.16305/j.1007-1334.2019. 11.017http://dx.doi.org/10.16305/j.1007-1334.2019.11.017.

周茜, 杨林, 张文静, 等. 薯蓣皂苷元对少弱精子症小鼠精子质量和生殖系统氧化应激损伤的影响[J]. 湖北文理学院学报, 2017, 38(5): 85-88. 10.3969/j.issn.1009-2854.2017.05.017http://dx.doi.org/10.3969/j.issn.1009-2854.2017.05.017

ZHOU Xi, YANG Lin, ZHANG Wenjing, et al. Assessment of sperm quality and oxidative stress injury of reproductive system after oligoasthenospermia mice received diosgenin[J]. Journal of Hubei University of Arts and Science, 2017, 38(5): 85-88. 10.3969/j.issn.1009-2854.2017.05.017http://dx.doi.org/10.3969/j.issn.1009-2854.2017.05.017

Peter C. Mann.大鼠和小鼠病理变化术语及诊断标准的国际规范（INHAND）[M]. 杨利峰, 周向梅, 赵德明, 主译. 北京: 中国农业出版社, 2019.

Peter C. Mann. International Specification for pathological terminology and diagnostic criteria in rats and mice (INHAND)[M]. YANG Lifeng, ZHOU Xiangmei, ZHAO Deming, chief translator. Beijing: China Agricultural Press, 2019.

颜贺欣, 夏玉军, 苏威. UCMSC对辐射损伤小鼠脾脏和肠道屏障防护作用[J]. 青岛大学医学院学报, 2016, 52(3): 291-293. DOI: 10.13361/j.qdyxy.201603011http://dx.doi.org/10.13361/j.qdyxy.201603011.

YAN Hexin, XIA Yujun, SU Wei. Barrier protection of umbilical cord mesenchymal stem cells on spleen and intestine of mouse with radiation damage[J]. Acta Academiae Medicinae Qingdao Universitatis, 2016, 52(3): 291-293. DOI: 10.13361/j.qdyxy.201603011http://dx.doi.org/10.13361/j.qdyxy.201603011.

Huang L M, Shi P, Zhang L J, et al. Compound Chinese medicine of anti-radiation protective effect of radiation injury in mice[J]. Chinese Pharmaceutical Affairs, 2011, 25(2): 132-134. DOI: 10.3402/mehd.v23i0.14787http://dx.doi.org/10.3402/mehd.v23i0.14787.

杨建鑫, 年永琼, 段雅彬, 等. 发酵冬虫夏草菌粉中虫草多糖的含量测定及其抗辐射作用初探[J]. 中国高原医学与生物学杂志, 2020, 41(1): 53-59. DOI: 10.13452/j.cnki.jqmc.2020.01.012http://dx.doi.org/10.13452/j.cnki.jqmc.2020.01.012.

YANG Jianxin, NIAN Yongqiong, DUAN Yabin, et al. Assay of polysaccharides in fermented cordyceps sinensis powder and anti-radiation effect of cordyceps sinensis polysaccharides[J]. Chinese High Altitude Medicine and Biology, 2020, 41(1): 53-59. DOI: 10. 13452/j.cnki.jqmc.2020.01.012http://dx.doi.org/10.13452/j.cnki.jqmc.2020.01.012.

Furlong H, Mothersill C, Lyng F M, et al. Apoptosis is signalled early by low doses of ionising radiation in a radiation-induced bystander effect[J]. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 2013, 741/742: 35-43. DOI: 10.1016/j.mrfmmm.2013.02.001http://dx.doi.org/10.1016/j.mrfmmm.2013.02.001.

Codenotti S, Faggi F, Ronca R, et al. Caveolin-1 enhances metastasis formation in a human model of embryonal rhabdomyosarcoma through Erk signaling cooperation[J]. Cancer Letters, 2019, 449: 135-144. DOI: 10.1016/j.canlet.2019.02.013http://dx.doi.org/10.1016/j.canlet.2019.02.013.

颜春鲁, 安方玉, 刘永琦, 等. 黄芪汤对重离子辐射(12C6+)所致大鼠放射性脑损伤的保护作用[J]. 辐射研究与辐射工艺学报, 2020, 38(6): 060301. DOI: 10. 11889/j.1000-3436.2020.rrj.38.060301http://dx.doi.org/10.11889/j.1000-3436.2020.rrj.38.060301.

YAN Chunlu, AN Fangyu, LIU Yongqi, et al. Protective effects of Huangqi decoction on heavy ion radiation (12C6+) injury of the rat brain[J]. Journal of Radiation Research and Radiation Processing, 2020, 38(6): 060301. DOI: 10.11889/j.1000-3436.2020.rrj.38.060301http://dx.doi.org/10.11889/j.1000-3436.2020.rrj.38.060301.

张超, 傅华, 蔡建明, 等. CpG-ODN对12C6+离子照射所致胸腺损伤的防治作用[J]. 辐射研究与辐射工艺学报, 2021, 39(1): 010302. DOI: 10.11889/j.1000-3436.2021.rrj.39.010302http://dx.doi.org/10.11889/j.1000-3436.2021.rrj.39.010302.

ZHANG Chao, FU Hua, CAI Jianming, et al. Effect of CpG-ODN on thymus damage radiated by carbon ions[J]. Journal of Radiation Research and Radiation Processing, 2021, 39(1): 010302. DOI: 10.11889/j.1000-3436.2021.rrj.39.010302http://dx.doi.org/10.11889/j.1000-3436.2021.rrj.39.010302.

文海若, 颜玉静, 王曼虹, 等. 基于彗星实验的大黄素型单蒽酮体内外毒性研究[J]. 中国现代中药, 2020, 22(10): 1644-1649. DOI: 10.13313/j.issn.1673-4890. 20191030001http://dx.doi.org/10.13313/j.issn.1673-4890.20191030001.

WEN Hairuo, YAN Yujing, WANG Manhong, et al. A toxicity study of monanthone with emodin type based on comet assay[J]. Modern Chinese Medicine, 2020, 22(10): 1644-1649. DOI: 10.13313/j.issn.1673-4890. 20191030001http://dx.doi.org/10.13313/j.issn.1673-4890.20191030001.

张续, 郑洁, 赵莉平, 等. 骨性关节炎中T细胞变化和作用的研究进展[J]. 中国免疫学杂志, 2022, 38(1): 107-112. DOI: 10.3969/j.issn.1000-484X.2022.01.020http://dx.doi.org/10.3969/j.issn.1000-484X.2022.01.020.

ZHANG Xu, ZHENG Jie, ZHAO Liping, et al. Research progress of T cell changes and functions in osteoarthritis[J]. Chinese Journal of Immunology, 2022, 38(1): 107-112. DOI: 10.3969/j.issn.1000-484X.2022.01.020http://dx.doi.org/10.3969/j.issn.1000-484X.2022.01.020.

Ratikan J A, Micewicz E D, Xie M W, et al. Radiation takes its toll[J]. Cancer Letters, 2015, 368(2): 238-245. DOI: 10.1016/j.canlet.2015.03.031http://dx.doi.org/10.1016/j.canlet.2015.03.031.

田宇, 赵冬梅, 尹玲玲, 等. 60Co γ射线致死剂量全身照射对小鼠脾脏和肠系膜淋巴结T细胞亚群表面免疫分子表达水平的影响[J]. 国际输血及血液学杂志, 2016, 39(1): 26-30. DOI: 10.3760/cma.j.issn.1673-419X.2016.01.005http://dx.doi.org/10.3760/cma.j.issn.1673-419X.2016.01.005.

TIAN Yu, ZHAO Dongmei, YIN Lingling, et al. Effects on expression levels of surface immune molecules on T cell subsets in spleens and mesenteric lymph node of mice irradiated by 60Co γ-ray lethally total body irradiation[J]. International Journal of Blood Transfusion and Hematology, 2016, 39(1): 26-30. DOI: 10.3760/cma.j.issn.1673-419X.2016.01.005http://dx.doi.org/10.3760/cma.j.issn.1673-419X.2016.01.005.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

CpG-ODN 对重离子辐射所致脾脏损伤的救治作用

5G手机辐射对小鼠精子质量和性激素水平的影响

草本组方对γ射线全身照射小鼠的保护作用

兰州百合多糖片段对X射线诱导的小鼠骨髓有核细胞损伤的保护作用