重离子束辐射诱导细胞双链断裂(DSBs)损伤及修复机理的研究进展

任军乐; 郭晓鹏; 雷彩荣; 张苗苗; 柴冉; 陆栋

doi:10.11889/j.1000-3436.2022-0081

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重离子束辐射诱导细胞双链断裂(DSBs)损伤及修复机理的研究进展

综述 | 更新时间：2023-06-21

- 重离子束辐射诱导细胞双链断裂(DSBs)损伤及修复机理的研究进展
- Progress of research on double-strand break damage induced by heavy-ion beam radiation and repair mechanism
- 辐射研究与辐射工艺学报 2023年41卷第3期页码：30101
- 作者机构：
  
  1.中国科学院近代物理研究所兰州730000
  2.中国科学院大学北京100049
  3.兰州理工大学兰州 730050
  4.甘肃省微生物资源开发利用重点实验室兰州 730070
- 作者简介：
  
  任军乐，男，1996年9月出生，2021年6月毕业于兰州理工大学，现为中国科学院近代物理研究所生物物理学硕士研究生，E-mail: rjl17393128264@163.com
  陆栋，博士生导师，E-mail: ld@impcas.ac.cn
- 基金信息：
  
  本项目由国家自然科学基金项目(11905265;11975284);甘肃省自然科学基金项目(20JR5RA552);中国科学院“西部青年学者”(E023211Y)
- DOI：10.11889/j.1000-3436.2022-0081
  中图分类号：
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任军乐, 郭晓鹏, 雷彩荣, 等. 重离子束辐射诱导细胞双链断裂(DSBs)损伤及修复机理的研究进展[J]. 辐射研究与辐射工艺学报, 2023,41(3):030101.

REN Junle, GUO Xiaopeng, LEI Cairong, et al. Progress of research on double-strand break damage induced by heavy-ion beam radiation and repair mechanism[J]. Journal of Radiation Research and Radiation Processing, 2023,41(3):030101.
任军乐, 郭晓鹏, 雷彩荣, 等. 重离子束辐射诱导细胞双链断裂(DSBs)损伤及修复机理的研究进展[J]. 辐射研究与辐射工艺学报, 2023,41(3):030101. DOI： 10.11889/j.1000-3436.2022-0081.

REN Junle, GUO Xiaopeng, LEI Cairong, et al. Progress of research on double-strand break damage induced by heavy-ion beam radiation and repair mechanism[J]. Journal of Radiation Research and Radiation Processing, 2023,41(3):030101. DOI： 10.11889/j.1000-3436.2022-0081.

摘要

重离子束辐射能引发细胞DNA双链断裂，被认为是构成基因组不稳定因素之一。现有研究表明：同源末端连接、同源重组、单链退火和选择性末端连接在修复DNA双链断裂方面发挥着重要的作用，但是影响DNA双链断裂修复途径选择的因素目前仍不清楚。本文对近年重离子辐射细胞产生的DNA损伤特征和修复途径方面的新发现进行了综述，并从类型和分布、染色质状态、DNA末端结构、DNA末端切除、细胞周期方面解释了细胞DNA双链断裂修复途径的选择机制。这对细胞DNA损伤修复的研究具有重要意义，为重离子辐射技术在生物学效应研究方面提供了参考。

Abstract

Heavy-ion beam radiation can cause cell DNA double-strand breaks (DSBs), which are factors that lead to genomic instability. Existing studies have demonstrated that homologous end joining, homologous recombination, single-strand annealing, and selective end joining play critical roles in the repair of DNA DSBs. However, the factors that affect the selection of repair pathways for DNA DSBs remain unclear. In this study, recent findings on DNA damage characteristics and repair pathways generated by heavy-ion radiation cells are reviewed, and the selection mechanism of DSB repair pathways in cells is explained in terms of the types and distribution of DNA DSBs, chromatin status, DNA terminal structures, DNA terminal excision, and cell cycles. This review is of great significance for the study of DNA damage repair and provides a reference for investigating the biological effects of heavy-ion radiation technology.

关键词

重离子束辐射DNA损伤簇状DSBsDNA损伤修复

Keywords

Heavy-ion beam radiationDNA damageClustered DSBsDNA damage repair

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