电子束辐照对不同含水量芦苇木质纤维素结构及酶解性能的影响
Effects of electron beam irradiation pretreatment on the structure and enzymatic efficiency of
Phragmites australis lignocelluloses with different moisture contents- 辐射研究与辐射工艺学报 2023年41卷第2期 页码：20401
- 作者机构：
1.湖南省农业科学院 湖南省核农学与航天育种研究所/湖南省农业生物辐照工程技术研究中心 长沙 410125
2.湖南大学隆平分院 长沙 410125
- 作者简介：
陈亮，男，1981年9月出生，2007年于湖南农业大学农产品加工及贮藏工程专业获工学硕士学位，现从事核农学研究工作，副研究员，E-mail: chenliang912@163.com
王克勤，研究员，E-mail: wkq6412@163.com
- 基金信息：国家自然科学基金项目(12005058);湖南省农业科技创新资金项目(2022CX98)
DOI：10.11889/j.1000-3436.2022-0105
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陈亮, 武小芬, 齐慧, 等. 电子束辐照对不同含水量芦苇木质纤维素结构及酶解性能的影响[J]. 辐射研究与辐射工艺学报, 2023,41(2):020401.
CHEN Liang, WU Xiaofen, QI Hui, et al. Effects of electron beam irradiation pretreatment on the structure and enzymatic efficiency of
Phragmites australis lignocelluloses with different moisture contents[J]. Journal of Radiation Research and Radiation Processing, 2023,41(2):020401.陈亮, 武小芬, 齐慧, 等. 电子束辐照对不同含水量芦苇木质纤维素结构及酶解性能的影响[J]. 辐射研究与辐射工艺学报, 2023,41(2):020401. DOI： 10.11889/j.1000-3436.2022-0105.
CHEN Liang, WU Xiaofen, QI Hui, et al. Effects of electron beam irradiation pretreatment on the structure and enzymatic efficiency of
Phragmites australis lignocelluloses with different moisture contents[J]. Journal of Radiation Research and Radiation Processing, 2023,41(2):020401. DOI： 10.11889/j.1000-3436.2022-0105.
摘要
以不同含水量的芦苇木质纤维素（以下简称芦苇）为试验材料，采用5 MeV电子束辐照处理，研究了电子束辐照对不同含水量芦苇化学组分、超分子结构、粉碎粒径及酶解性能的影响。结果表明：不同含水量的芦苇经电子束辐照后均发生降解，超分子结构受到破坏，粉碎后小粒径颗粒显著增加，酶解转化率大幅度提高；当吸收剂量相同时，不同含水量芦苇的表观形貌、超分子结构及粉碎粒径分布没有明显区别，但酶解转化率与含水量呈负相关，含水量5%、10%和50%的芦苇经过500 kGy 电子束辐照后，纤维素酶解转化率分别22.24%、19.76%和18.57%，半纤维素酶解转化率分别为25.04%、23.84%和19.56%，经过1 000 kGy电子束辐照后，含水量5%、10%和50%的芦苇纤维素酶解转化率分别为54.09%、47.27%和49.24%，半纤维素酶解转化率分别62.30%、53.25%和47.83%；当吸收剂量为500 kGy时，含水量为5%的芦苇纤维素、半纤维素较含水量10%和50%芦苇降解严重，而当吸收剂量为1 000 kGy时，含水量为50%的芦苇纤维素、半纤维素则较含水量5%和10%的芦苇降解严重，并且较含水量5%和10%的芦苇，含水量为50%的芦苇纤维素、半纤维素更多地被降解为非糖类物质。
Abstract
The study investigated the chemical composition, supramolecular structures, particle size distribution, and enzymatic efficiency of ,Phragmites australis, lignocelluloses (PALs) with different moisture contents and treatment with 5 MeV electron beam irradiation. After irradiation, regardless of the moisture content, all PAL samples were degraded, their supramolecular structures were damaged, and the proportion of small PAL particles and the PAL enzymatic conversion rate increased significantly. There was no obvious difference in the apparent morphology, chemical structure, or crushed particle size distribution of PAL samples with different moisture contents when the absorbed dose was the same, but the enzymatic efficiency was negatively correlated with moisture content. The cellulose enzymatic conversion rates of PAL samples with 5%, 10%, and 50% moisture content were 22.24%, 19.76%, and 18.57%, respectively, and the enzymatic conversion rates of hemicellulose were 25.04%, 23.84%, and 19.56%, respectively, after 500 kGy electron beam irradiation. Meanwhile, the cellulose enzymatic conversion rates of PAL with 5%, 10%, and 50% moisture content were 54.09%, 47.27%, and 49.24%, respectively, and the enzymatic conversion rates of hemicellulose were 62.30%, 53.25%, and 47.83%, respectively, after 1 000 kGy electron beam irradiation. When the absorbed dose was 500 kGy, PAL cellulose and hemicellulose samples with 5% moisture content were severely degraded compared to samples with 10% and 50%. Meanwhile, when the absorbed dose was 1 000 kGy, the PAL cellulose and hemicellulose samples with 50% moisture content were severely degraded compared to those with 5% and 10% moisture content. Moreover, more cellulose and hemicellulose with 50% moisture content in PAL were degraded to non-carbohydrate substances compared to those with 5% and 10% moisture content.
关键词
电子束辐照含水量芦苇木质纤维素结构酶解
Keywords
Electron beamIrradiationMoisture contentPhragmites australisLignocellulosesStructureEnzymatic hydrolysis
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