SC双波源脉冲微波相互垂直复合辐照对大鼠重要组织器官生物效应的影响
Effects of SC dual pulsed-microwaves irradiation on the bioeffects in rats' organs under a mutually perpendicular incidence
- 辐射研究与辐射工艺学报 2023年41卷第2期 页码：20301
- 作者机构：
1.华东师范大学物理与电子科学学院 上海磁共振重点实验室 上海 200241
- 作者简介：
叶金怡，女，1997年1月出生，2019年6月毕业于浙江师范大学，现为华东师范大学在读硕士研究生，无线电物理专业
王向晖，博士，副教授，E-mail: xhwang@phy.ecnu.edu.cn
- 基金信息：国家自然科学基金项目(31600675)
DOI：10.11889/j.1000-3436.2022-0109
中图分类号：
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叶金怡, 张杰, 齐红新, 等. SC双波源脉冲微波相互垂直复合辐照对大鼠重要组织器官生物效应的影响[J]. 辐射研究与辐射工艺学报, 2023,41(2):020301.
YE Jinyi, ZHANG Jie, QI Hongxin, et al. Effects of SC dual pulsed-microwaves irradiation on the bioeffects in rats' organs under a mutually perpendicular incidence[J]. Journal of Radiation Research and Radiation Processing, 2023,41(2):020301.
叶金怡, 张杰, 齐红新, 等. SC双波源脉冲微波相互垂直复合辐照对大鼠重要组织器官生物效应的影响[J]. 辐射研究与辐射工艺学报, 2023,41(2):020301. DOI： 10.11889/j.1000-3436.2022-0109.
YE Jinyi, ZHANG Jie, QI Hongxin, et al. Effects of SC dual pulsed-microwaves irradiation on the bioeffects in rats' organs under a mutually perpendicular incidence[J]. Journal of Radiation Research and Radiation Processing, 2023,41(2):020301. DOI： 10.11889/j.1000-3436.2022-0109.
摘要
本文研究了S和C波段双波源沿相互垂直方向同时辐照时，脉冲数对大鼠血液、脑、睾丸、心脏和肝脏组织的影响，并与相同电磁暴露水平条件下的单波源电磁暴露生物效应进行了比较。结果表明：当空间场强幅值为44.84 kV/m，脉冲数为2、5、20和200时，辐照组大鼠的血常规、血清睾酮、谷丙转氨酶（Alanine aminotransferase，ALT）和谷草转氨酶（Aspartate aminotransferase，AST）含量等指标与Control组及Sham组相比均未发生明显变化（,p,>,0.05）。辐照组大鼠的脑、睾丸、心脏和肝脏组织形态正常，细胞核面积统计结果及睾丸组织的Johnson评分结果与Control及Sham组相比均无明显差异（,p,>,0.05），没有发现明显的病理损伤。辐照组大鼠4个组织中的促凋亡蛋白Bax的含量均随脉冲数增加呈非线性增加趋势，具有脉冲微波辐照在非热效应下的场效应特征。当脉冲数大于2时，脑和睾丸组织出现了明显的凋亡响应（,p,˂0.05），而心脏和肝脏组织在脉冲数达到20时才出现明显的凋亡响应（,p,˂0.05），这主要是因为脑和睾丸的内场场强幅值（分别为11.3 kV/m和10 kV/m）高于心脏和肝脏组织的内场场强幅值（分别为5.73 kV/m和5.33 kV/m）。在电磁暴露水平基本相同的情况下，复合辐照与两波源单独辐照所产生的凋亡响应之间无显著性差异（,p,>,0.05）。
Abstract
To investigate the potential risk of electromagnetic exposure, systematic studies on the bioeffects caused by single-frequency electromagnetic fields have been carried out with plenty of achievements.However, the bioeffects caused by multiple-frequency fields still need to be further studied. In the resent work, we constructed a dual-sourced irradiation system to allow two microwaves simultaneously incident from two perpendicular directions. Subsequently, the influence of the number of pulses on the blood, brain, testis, heart, and liver tissues of rats under the simultaneous irradiation of S-band and C-band pulsed microwaves was studied, and compared to that caused by the single-sourced exposure with the same electromagnetic exposure dose. The results showed that when the amplitude of the space electric field intensity was 44.84 kV/m and the number of the pulses was 2, 5, 20 and 200, the blood routine, testosterone, alanine aminotransferase and aspartate aminotransferase content of rats in the irradiation groups did not change significantly compared with the control group and sham group (,p,>,0.05). The nuclear area in the brain, heart and liver of rats, as well as the Johnson score of the testis, has not significantly changed after the irradiation (,p,>,0.05), and no pathological changes have been observed in these target tissues. The content of Bax in the above four tissues of rats in the irradiation groups increased nonlinearly with the increase of the number of pulses, which can be described as the non-thermal bioeffect that concerned the intensity of the electric field of the pulsed microwave. When the number of pulses is greater than 2, a significant apoptotic response was detected in the brain and testicle (,p,˂0.05), while in the liver and heart the pulse number needs to be greater than 20 (,p,˂0.05). This is mainly because the amplitude of the internal field of the brain and testicle (11.3 kV/m and 10 kV/m respectively) is higher than that of the liver and heart (5.73 kV/m and 5.33 kV/m respectively). Under the same electromagnetic exposure dose, there was no difference between the SC combined irradiation and the S or C individual-irradiation (,p,>,0.05).
关键词
双波源复合场电磁暴露生物效应比吸收能脉冲微波
Keywords
Dual-wave combined fieldElectromagnetic exposureBiological effectSpecific absorption energyPulsed microwave
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