CpG-ODN 对重离子辐射所致脾脏损伤的救治作用

张超; 傅华; 易永华; 蔡建明; 高福

doi:10.11889/j.1000-3436.2022-0116

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CpG-ODN 对重离子辐射所致脾脏损伤的救治作用

辐射生物学与医学 | 更新时间：2023-08-29

- CpG-ODN 对重离子辐射所致脾脏损伤的救治作用
- Effect of CpG-ODN on spleen damage after carbon ion radiation
- 辐射研究与辐射工艺学报 2023年41卷第4期页码：40301
- 作者机构：
  
  1.中国人民解放军联勤保障部队第九四〇医院放疗科兰州 730050
  2.中国人民解放军海军军医大学放射医学教研室上海 200433
- 作者简介：
  
  张超，男，1984年出生，2014获得海军军医大学医学博士学位，副主任医师，主要从事肿瘤放射治疗工作
  傅华，硕士，副主任医师，E-mail：47723515@qq.com
- 基金信息：
  
  甘肃省自然科学基金(22JR5RA003);国家自然科学基金项目(81501623)
- DOI：10.11889/j.1000-3436.2022-0116
  中图分类号：
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张超, 傅华, 易永华, 等. CpG-ODN 对重离子辐射所致脾脏损伤的救治作用[J]. 辐射研究与辐射工艺学报, 2023,41(4):040301.

ZHANG Chao, FU Hua, YI Yonghua, et al. Effect of CpG-ODN on spleen damage after carbon ion radiation[J]. Journal of Radiation Research and Radiation Processing, 2023,41(4):040301.
张超, 傅华, 易永华, 等. CpG-ODN 对重离子辐射所致脾脏损伤的救治作用[J]. 辐射研究与辐射工艺学报, 2023,41(4):040301. DOI： 10.11889/j.1000-3436.2022-0116.

ZHANG Chao, FU Hua, YI Yonghua, et al. Effect of CpG-ODN on spleen damage after carbon ion radiation[J]. Journal of Radiation Research and Radiation Processing, 2023,41(4):040301. DOI： 10.11889/j.1000-3436.2022-0116.

摘要

本次研究主要探讨CpG-ODN对重离子辐射所致脾脏损伤的救治作用。实验中C57BL/6L小鼠受到5 Gy ,12,C,6+,离子全身照射，在照射后急性期计算小鼠脾脏指数，苏木精-伊红（Hematoxylin-Eosin staining，HE）染色观察脾脏组织结构变化。另外，免疫组化法检测γ-H,2,AX蛋白含量以判断脾脏细胞DNA发生双链断裂（（Double-strand break，DSB）情况，末端脱氧核苷酸转移酶介导的dUTP缺口末端标（Terminal deoxynucleotidyl transferase-mediated dUTP nick and labeling，TUNEL）法检测脾脏细胞凋亡水平，荧光标记法检测外周血CD19,+,B细胞数量。结果显示，经,12,C,6+,离子照射后CpG-ODN处理，小鼠脾脏指数提高，脾脏红髓萎缩程度减轻，白髓面积增大，其内淋巴B细胞排列密度提高，发生DSB和凋亡的细胞数量减少，外周血CD19,+, B细胞数量增加。这些表明CpG-ODN能减轻,12,C,6+,离子照射后脾脏损伤，其原因可能与CpG-ODN抑制细胞发生DSB和凋亡有关。

Abstract

The purpose of this study was to explore the ameliorative effect of CpG-ODN on spleen damage after carbon ion radiation (CIR). In the experiment, C57BL/6L mice were irradiated with 5 Gy of CIR and the index of spleen was calculated. Changes in spleen tissue structure were observed by histological examination (HE). Double-strand break (DSB) of DNA and apoptosis were detected by γ-H,2,AX immunohistochemistry and terminal deoxynucleotidyl transferase-mediated dUTP nick and labeling (TUNEL) assay, respectively. The number of B cells in the peripheral blood was examined using CD19 antibody labeling. These results showed that CpG-ODN improved the index of spleen, relieved red pulp atrophy, and increased the area of white pulp, in which the number of γ-H,2,AX foci and TUNEL-positive cells were decreased after CIR. In addition, CpG-OND increased the number of CD19,+, B cells in peripheral blood after CIR. These results indicated that CpG-ODN ameliorates spleen damage induced by CIR, possibly by inhibiting the rate of DSB and apoptosis.

关键词

碳离子辐射CpG-ODN脾脏凋亡双链断裂

Keywords

Carbon ion radiation (CIR)CpG-ODNSpleenApoptosisDouble-strand break (DSB)

references

王姗, 李萍, 张芮, 等. 重离子治疗肿瘤的临床研究进展[J]. 国际肿瘤学杂志, 2020, 47(12): 741-745. DOI: 10.3760/cma.j.cn371439-20200117-00112http://dx.doi.org/10.3760/cma.j.cn371439-20200117-00112.

WANG Shan, LI Ping, ZHANG Rui, et al. Progresses in clinical research of heavy ion therapy for cancer[J]. Journal of International Oncology, 2020, 47(12): 741-745. DOI: 10.3760/cma.j.cn371439-20200117-00112http://dx.doi.org/10.3760/cma.j.cn371439-20200117-00112.

杨红杰, 肖平, 罗乐宣, 等. 重离子治疗恶性肿瘤的生物学基础[J]. 罕少疾病杂志, 2015, 22(1): 59-62. DOI: 10. 3969/j.issn.1009-3257.2015.01.24http://dx.doi.org/10.3969/j.issn.1009-3257.2015.01.24.

YANG Hongjie, XIAO Ping, LUO Lexuan, et al. The biological basis of heavy ion for the therapy of malignant tumor[J]. Journal of Rare and Uncommon Diseases, 2015, 22(1): 59-62. DOI: 10.3969/j.issn.1009-3257. 2015.01.24http://dx.doi.org/10.3969/j.issn.1009-3257.2015.01.24.

Suetens A, Konings K, Moreels M, et al. Higher initial DNA damage and persistent cell cycle arrest after carbon ion irradiation compared to X-irradiation in prostate and colon cancer cells[J]. Frontiers in Oncology, 2016, 6: 87. DOI: 10.3389/fonc.2016.00087http://dx.doi.org/10.3389/fonc.2016.00087.

查晓丹, 李小满, 蒲韵竹, 等. 重离子辐射对免疫系统影响的研究进展[J]. 国际药学研究杂志, 2015, 42(2): 152-155. DOI: 10.13220/j.cnki.jipr.2015.02.005http://dx.doi.org/10.13220/j.cnki.jipr.2015.02.005.

ZHA Xiaodan, LI Xiaoman, PU Yunzhu, et al. Effects of heavy ion radiation on immune system: research advances[J]. Journal of International Pharmaceutical Research, 2015, 42(2): 152-155. DOI: 10.13220/j.cnki.jipr.2015. 02.005http://dx.doi.org/10.13220/j.cnki.jipr.2015.02.005.

崔玉芳, 杨红, 高亚兵, 等. 小鼠脾淋巴细胞辐射死亡与凋亡之间的关系[J]. 辐射研究与辐射工艺学报, 1999, 17(2): 101-106. DOI: 10.3969/j.issn.1000-3436.1999. 02.008http://dx.doi.org/10.3969/j.issn.1000-3436.1999.02.008.

CUI Yufang, YANG Hong, GAO Yabing, et al. Role of apoptosis in radiation-induced mouse spleen damage and repair and its mechanism[J]. Journal of Radiation Research and Radiation Processing, 1999, 17(2): 101-106. DOI: 10.3969/j.issn.1000-3436.1999.02.008http://dx.doi.org/10.3969/j.issn.1000-3436.1999.02.008.

赵卫平, 张红, 王燕玲, 等. 低剂量12C6+离子辐照对小鼠胸腺、脾脏细胞周期及DNA损伤的影响[J]. 辐射研究与辐射工艺学报, 2008, 26(6): 343-347. DOI: 10.3969/j.issn.1000-3436.2008.06.005http://dx.doi.org/10.3969/j.issn.1000-3436.2008.06.005.

ZHAO Weiping, ZHANG Hong, WANG Yanling, et al. Effect of low dose 12C6+ irradiation on cell cycle and DNA damage in mouse thymus and spleen cells[J]. Journal of Radiation Research and Radiation Processing, 2008, 26(6): 343-347. DOI: 10.3969/j.issn.1000-3436.2008. 06.005http://dx.doi.org/10.3969/j.issn.1000-3436.2008.06.005.

Lenert P, Stunz L, Yi A K, et al. CpG stimulation of primary mouse B cells is blocked by inhibitory oligodeoxyribonucleotides at a site proximal to NF-κB activation[J]. Antisense and Nucleic Acid Drug Development, 2001, 11(4): 247-256. DOI: 10.1089/108729001317022241http://dx.doi.org/10.1089/108729001317022241.

王吉超, 张超, 蔡建明. CpG-ODN对辐射所致人小肠隐窝上皮细胞微核形成的影响[J]. 辐射研究与辐射工艺学报, 2011, 29(3): 159-162.

WANG Jichao, ZHANG Chao, CAI Jianming. Effect of CpG-ODN combined with radiation on micronuclei cell of the human intestinal crypt epithelial cell[J]. Journal of Radiation Research and Radiation Processing, 2011, 29(3): 159-162.

Zhang C, Zheng M, Zhu X H, et al. Protective effect of CpG-oligodeoxynucleotides against low- and high-LET irradiation[J]. Cellular Physiology and Biochemistry: International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology, 2014, 34(5): 1663-1674. DOI: 10.1159/000366368http://dx.doi.org/10.1159/000366368.

张超, 傅华, 蔡建明, 等. CpG-ODN对12C6+离子照射所致胸腺损伤的防治作用[J]. 辐射研究与辐射工艺学报, 2021, 39(1): 010302. DOI: 10.11889/j.1000-3436.2021.rrj.39.010302http://dx.doi.org/10.11889/j.1000-3436.2021.rrj.39.010302.

ZHANG Chao, FU Hua, CAI Jianming, et al. Effect of CpG-ODN on thymus damage radiated by carbon ions[J]. Journal of Radiation Research and Radiation Processing, 2021, 39(1): 010302. DOI: 10.11889/j.1000-3436.2021.rrj.39.010302http://dx.doi.org/10.11889/j.1000-3436.2021.rrj.39.010302.

Suman S, Datta K, Trani D, et al. Relative biological effectiveness of 12C and 28Si radiation in C57BL/6J mice[J].Radiation and Environmental Biophysics, 2012, 51(3): 303-309. DOI: 10.1007/s00411-012-0418-9http://dx.doi.org/10.1007/s00411-012-0418-9.

张睿凤, 张忠新, 党旭红, 等. 重离子生物效应的研究进展[J]. 国际放射医学核医学杂志, 2016, 40(4): 306-311. DOI: 10.3760/cma.j.issn.1673-4114.2016.04.014http://dx.doi.org/10.3760/cma.j.issn.1673-4114.2016.04.014.

ZHANG Ruifeng, ZHANG Zhongxin, DANG Xuhong, et al. Progress on biological effectiveness of heavy ions[J]. International Journal of Radiation Medicine and Nuclear Medicine, 2016, 40(4): 306-311. DOI: 10.3760/cma.j.issn.1673-4114.2016.04.014http://dx.doi.org/10.3760/cma.j.issn.1673-4114.2016.04.014.

Benezra M, Chevallier N, Morrison D J, et al. BRCA1 augments transcription by the NF-kappa B transcription factor by binding to the Rel domain of the p65/RelA subunit[J]. The Journal of Biological Chemistry, 2003, 278(29): 26333-26341. DOI: 10.1074/jbc.M303076200http://dx.doi.org/10.1074/jbc.M303076200.

Guglielmi C, Cerri I, Evangelista M, et al. Identification of two novel BRCA1-partner genes in the DNA double-strand break repair pathway[J]. Breast Cancer Research and Treatment, 2013, 141(3): 515-522. DOI: 10.1007/s10549-013-2705-9http://dx.doi.org/10.1007/s10549-013-2705-9.

Sommariva M, De Cecco L, Tagliabue E, et al. Modulation of DNA repair genes induced by TLR9 agonists: a strategy to eliminate altered cells?[J]. Oncoimmunology, 2012, 1(2): 258-259. DOI: 10.4161/onci.1.2.18343http://dx.doi.org/10.4161/onci.1.2.18343.

Zhang C, Ni J, Gao F, et al. The mechanism for the ameliorative effect of CpG-oligodeoxynucleotides on bone marrow hemopoiesis radiation injury[J]. Basic & Clinical Pharmacology & Toxicology, 2011, 109(1): 11-16. DOI: 10.1111/j.1742-7843.2011.00695.xhttp://dx.doi.org/10.1111/j.1742-7843.2011.00695.x.

Hada M, Georgakilas A G. Formation of clustered DNA damage after high-LET irradiation: a review[J]. Journal of Radiation Research, 2008, 49(3): 203-210. DOI: 10. 1269/jrr.07123http://dx.doi.org/10.1269/jrr.07123.

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