川芎指纹图谱的建立及60Co-γ辐照对其有效成分的影响
Establishing the fingerprints of
Ligusticum chuanxiong and assessing the effects of 60Co-γ irradiation on its active components- 辐射研究与辐射工艺学报 2023年41卷第5期 页码：50303
- 作者机构：
1.四川省原子能研究院 成都 610101
2.辐照保藏四川省重点实验室 成都 610101
3.成都理工大学，核技术与自动化工程学院 成都 610059
- 作者简介：
邱娅璐，女，1995年8月出生，2021年6月于黑龙江大学获硕士学位，实习研究员
高鹏，硕士，副研究员，E-mail: ppenggao@163.com
- 基金信息：四川省科技创新苗子工程项目(2021JDRC0121;2022JDRC0120);四川省自然科学基金面上项目(2022NSFSC0593);四川省区域创新合作(2022YFQ0044);四川省科技创新人才项目(2021JDRC0042);成都市龙泉驿区科技计划项目(2022-34-2)
DOI：10.11889/j.1000-3436.2023-0007
中图分类号：
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邱娅璐, 高鹏, 何江, 等. 川芎指纹图谱的建立及60Co-γ辐照对其有效成分的影响[J]. 辐射研究与辐射工艺学报, 2023,41(5):050303.
QIU Yalu, GAO Peng, HE Jiang, et al. Establishing the fingerprints of
Ligusticum chuanxiong and assessing the effects of 60Co-γ irradiation on its active components[J]. Journal of Radiation Research and Radiation Processing, 2023,41(5):050303.邱娅璐, 高鹏, 何江, 等. 川芎指纹图谱的建立及60Co-γ辐照对其有效成分的影响[J]. 辐射研究与辐射工艺学报, 2023,41(5):050303. DOI： 10.11889/j.1000-3436.2023-0007.
QIU Yalu, GAO Peng, HE Jiang, et al. Establishing the fingerprints of
Ligusticum chuanxiong and assessing the effects of 60Co-γ irradiation on its active components[J]. Journal of Radiation Research and Radiation Processing, 2023,41(5):050303. DOI： 10.11889/j.1000-3436.2023-0007.
摘要
为了建立川芎指纹图谱，考察不同剂量,60,Co-γ辐照对川芎8种有效成分的影响，以川芎为材料，采用SHIMADZU Shim-pack GIST C18色谱柱（250 mm×4.6 mm，5 μm）对8种有效成分的标准品和对照品储备液进行分析。设定不同剂量（3 kGy、6 kGy、10 kGy、15 kGy）,60,Co-γ辐照处理川芎，处理后样品通过液相色谱法得到指纹图谱。采用《中药色谱指纹图谱相似度评价系统（2004A版）》对相似度进行评价。结果表明：川芎各成分相似度均大于0.988；在低于10 kGy的射线辐照处理后，川芎的8种有效成分含量无明显差异。结果表明该方法稳定可靠。该研究为辐照技术在川芎品质保障及川芎药材辐照标准的制定提供理论依据和支撑。
Abstract
Herein, we established the fingerprint of ,Ligusticum chuanxiong, and the effects of different ,60,Co-γ irradiation doses on the eight active components of ,Ligusticum chuanxiong, were investigated. A stock solution of the eight standard reference substances in ,Ligusticum chuanxiong, were analyzed using a SHIMADZU Shim-pack GIST C18 chromatographic column (250 mm × 4.6 mm, 5 μm). Different doses (3 kGy, 6 kGy,10 kGy and 15 kGy) of ,60,Co-γ irradiation were used to treat ,Ligusticum chuanxiong,. After these treatments, the fingerprint of ,Ligusticum chuanxiong, was obtained via liquid chromatography. The similarity was evaluated using the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (2004 A edition), and the results indicated that the similarity of each component in ,Ligusticum chuanxiong, was ,>,0.988. After irradiation at ,<,10 kGy, the contents of the eight active components in ,Ligusticum chuanxiong, exhibited no evident differences, and the results indicated the stability and reliability of the method. Thus, this study provides a theoretical basis and support for the use of irradiation technology in the quality assurance of ,Ligusticum chuanxiong, and the formulation of irradiation standards for ,Ligusticum chuanxiong,.
关键词
川芎60Co-γ辐照有效成分指纹图谱
Keywords
Ligusticum chuanxiong60Co-γ irradiationActive ingredientsFingerprints
references
廖宇娇, 敖明月, 李星, 等. 不同炮制方法对川芎中化学成分的影响研究[J]. 中国中药杂志, 2021, 46(2): 374-379. DOI: 10.19540/j.cnki.cjcmm.20201022.303http://dx.doi.org/10.19540/j.cnki.cjcmm.20201022.303.
LIAO Yujiao, AO Mingyue, LI Xing, et al. Study on effect of different processing methods on chemical components of Chuanxiong rhizoma[J]. China Journal of Chinese Materia Medica, 2021, 46(2): 374-379. DOI: 10.19540/j.cnki.cjcmm.20201022.303http://dx.doi.org/10.19540/j.cnki.cjcmm.20201022.303.
刘尹, 郭伦锋, 郑龙. 不同产地川芎UPLC指纹图谱研究[J]. 贵州医药, 2020, 44(11): 1699-1701. DOI: 10.3969/j.issn.1000-744X.2020.11.006http://dx.doi.org/10.3969/j.issn.1000-744X.2020.11.006.
LIU Yin, GUO Lunfeng, ZHENG Long. UPLC fingerprint analysis of Chuanxiong rhizoma from different habitats[J]. Guizhou Medical Journal, 2020, 44(11): 1699-1701. DOI:10.3969/j.issn.1000-744X.2020.11.006http://dx.doi.org/10.3969/j.issn.1000-744X.2020.11.006.
张培, 赵林华, 张海宇, 等. 川芎临床应用及其用量[J]. 吉林中医药, 2019, 39(3): 309-312. DOI: 10.13463/j.cnki.jlzyy.2019.03.009http://dx.doi.org/10.13463/j.cnki.jlzyy.2019.03.009.
ZHANG Pei, ZHAO Linhua, ZHANG Haiyu, et al. Exploration about the clinical application and dosage of the rhizome of Chuanxiong[J]. Jilin Journal of Chinese Medicine, 2019, 39(3): 309-312. DOI: 10.13463/j.cnki.jlzyy.2019.03.009http://dx.doi.org/10.13463/j.cnki.jlzyy.2019.03.009.
李芊, 吴效科. 川芎化学成分及药理作用研究新进展[J]. 化学工程师, 2020, 34(1): 62-64. DOI: 10.16247/j.cnki.23-1171/tq.20200162http://dx.doi.org/10.16247/j.cnki.23-1171/tq.20200162.
LI Qian, WU Xiaoke. New progress in research on chemical constituents and pharmacological action of Ligusticum chuanxiong Hort[J]. Chemical Engineer, 2020, 34(1): 62-64. DOI: 10.16247/j.cnki.23-1171/tq.20200162http://dx.doi.org/10.16247/j.cnki.23-1171/tq.20200162.
Wang J, Wang L, Zhou H, et al. The isolation, structural features and biological activities of polysaccharide from Ligusticum chuanxiong: a review[J]. Carbohydrate Polymers, 2022, 285: 118971. DOI: 10.1016/j.carbpol. 2021.118971http://dx.doi.org/10.1016/j.carbpol.2021.118971.
Chen L, Hou J A, Deng W, et al. Quality comparison of traditional Chuanxiong produced in Dujiangyan City and Sichuan ProvinceC and Chuanxiong from other areas, based on analysis of volatile oil, total alkaloids and total ferulic acid contents[J]. Tropical Journal of Pharmaceutical Research, 2020, 19(12): 2659-2666. DOI: 10.4314/tjpr.v19i12.26http://dx.doi.org/10.4314/tjpr.v19i12.26.
王恒飞, 韩燕星, 蒋建东, 等. 植物中药有效成分抗血栓作用的研究进展[J]. 中国医药生物技术, 2022, 17(2): 145-149. DOI: 10.3969/j.issn.1673-713X.2022.02.008http://dx.doi.org/10.3969/j.issn.1673-713X.2022.02.008.
WANG Hengfei, HAN Yanxing, JIANG Jiandong, et al. Research progress on antithrombotic effect of effective components of traditional Chinese medicine[J]. Chinese Medicinal Biotechnology, 2022, 17(2): 145-149. DOI: 10.3969/j.issn.1673-713X.2022.02.008http://dx.doi.org/10.3969/j.issn.1673-713X.2022.02.008.
鄢玉芬, 徐双美, 梁乙川, 等. HPLC法测定不同贮藏条件下川芎中10种化学成分的含量[J]. 中国药房, 2019, 30(6): 807-812. DOI: 10.6039/j.issn.1001-0408.2019. 06.17http://dx.doi.org/10.6039/j.issn.1001-0408.2019.06.17.
YAN Yufen, XU Shuangmei, LIANG Yichuan, et al. Content determination of 10 kinds of chemical components in Ligusticum chuanxiong under different storage conditions by HPLC[J]. China Pharmacy, 2019, 30(6): 807-812. DOI: 10.6039/j.issn.1001-0408.2019. 06.17http://dx.doi.org/10.6039/j.issn.1001-0408.2019.06.17.
郑潇潇, 李泞汐, 余梅, 等. 虫蛀川芎挥发油成分GC-MS研究[J].中药与临床, 2017, 8(3): 7-10. DOI: CNKI:SUN:LCZY.0.2017-03-003http://dx.doi.org/CNKI:SUN:LCZY.0.2017-03-003.
ZHENG Xiaoxiao, LI Linxi, YU Mei, et al. Research on the component in volatile oil in Chuanxiong damaged by worms[J]. Pharmacy and Clinics of Chinese Materia Medica, 2017, 8(3): 7-10. DOI: CNKI:SUN:LCZY. 0.2017-03-003http://dx.doi.org/CNKI:SUN:LCZY.0.2017-03-003.
廖鑫, 王适, 蔡媛, 等. 基于知识图谱分析中药灭菌未来发展趋势[J]. 辐射研究与辐射工艺学报, 2022, 40(6): 060401. DOI: 10.11889/j.1000-3436.2022-0036http://dx.doi.org/10.11889/j.1000-3436.2022-0036.
LIAO Xin, WANG Shi, CAI Yuan, et al. Knowledge graph analysis of current situation and trend in Chinese medicine sterilization[J]. Journal of Radiation Research and Radiation Processing, 2022, 40(6): 060401. DOI: 10.11889/j.1000-3436.2022-0036http://dx.doi.org/10.11889/j.1000-3436.2022-0036.
国家药典委员会. 关于对《中国药典》2020年版微生物通则草案(一) 公开征求意见的通知[EB/OL]. (2018-02-26) [2023-03-26]. https://www.chp.org.cn/gjyjw/gzwj/3797.jhtmlhttps://www.chp.org.cn/gjyjw/gzwj/3797.jhtml.
Chinese Pharmacopoeia Commission. Notice on public solicitation for comments on the draft general principles of microbiology (一) of the Chinese Pharmacopoeia 2020 Edition[EB/OL]. (2018-02-26) [2023-03-26]. https://www.chp.org.cn/gjyjw/gzwj/3797.jhtmlhttps://www.chp.org.cn/gjyjw/gzwj/3797.jhtml.
崔永伟. 60Co-γ射线辐照灭菌对白芍药材中有效成分的影响[J]. 食品与药品, 2022, 24(1): 64-70. DOI: 10.3969/j.issn.1672-979X.2022.01.014http://dx.doi.org/10.3969/j.issn.1672-979X.2022.01.014.
CUI Yongwei. Effect of 60Co-γ radiant sterilization on effective components in paeonia lactiflora pall[J]. Food and Drug, 2022, 24(1): 64-70. DOI: 10.3969/j.issn.1672-979X.2022.01.014http://dx.doi.org/10.3969/j.issn.1672-979X.2022.01.014.
张乐佳, 李黎明, 宋军, 等. 60Co-γ射线辐照灭菌对鸡内金生药粉质量影响的研究[J]. 中南药学, 2020, 18(9): 1546-1550. DOI: 10.7539/j.issn.1672-2981.2020.09.020http://dx.doi.org/10.7539/j.issn.1672-2981.2020.09.020.
ZHANG Lejia, LI Liming, SONG Jun, et al. Effect of 60Co-γ ray irradiation on the quality of endothelium corneum raw powder[J]. Central South Pharmacy, 2020, 18(9): 1546-1550. DOI: 10.7539/j.issn.1672-2981.2020. 09.020http://dx.doi.org/10.7539/j.issn.1672-2981.2020.09.020.
曹雨晴, 楚尧娟, 刘克锋, 等. 我国中药指纹图谱研究的可视化分析[J]. 世界科学技术-中医药现代化, 2020, 22(9): 3073-3081. DOI: 10.11842/wst.20191217002http://dx.doi.org/10.11842/wst.20191217002.
CAO Yuqing, CHU Yaojuan, LIU Kefeng, et al. Visual analysis on fingerprint studies of Chinese materia medica in China[J]. Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology, 2020, 22(9): 3073-3081. DOI: 10.11842/wst.20191217002http://dx.doi.org/10.11842/wst.20191217002.
肖雪霞, 陈一村. 不同产地蘡薁的高效液相色谱指纹图谱研究[J]. 汕头大学医学院学报, 2023, 36(1): 34-39. DOI: 10.13401/j.cnki.jsumc.2023.01.008http://dx.doi.org/10.13401/j.cnki.jsumc.2023.01.008.
XIAO Xuexia, CHEN Yicun. High performance liquid chromatography fingerprint of Vitis adstricta Hance from different origins[J]. Journal of Shantou University Medical College, 2023, 36(1): 34-39. DOI: 10.13401/j.cnki.jsumc.2023.01.008http://dx.doi.org/10.13401/j.cnki.jsumc.2023.01.008.
蔡宝昌, 潘扬, 王天山, 等. 中药川芎指纹图谱共有模式的建立及其在药材质量控制中的应用[J]. 世界科学技术-中医药现代化. 2002, 4(6): 41-45. DOI: 10.3969/j.issn.1674-3849.2002.06.011http://dx.doi.org/10.3969/j.issn.1674-3849.2002.06.011.
CAI Baochang, PAN Yang, WANG Tianshan, et al. Establishment of mutual mode of chromatographic fingerprint and its application to quality control of Chuanxiong[J]. Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology, 2002, 4(6): 41-45. DOI: 10.3969/j.issn.1674-3849.2002.06.011http://dx.doi.org/10.3969/j.issn.1674-3849.2002.06.011.
石海培, 包贝华, 黄胜良, 等. 川芎饮片的HPLC指纹图谱建立、聚类分析及偏最小二乘判别分析[J]. 中国药房, 2019, 30(8): 1066-1071. DOI: 10.6039/j.issn.1001-0408.2019.08.11http://dx.doi.org/10.6039/j.issn.1001-0408.2019.08.11.
SHI Haipei, BAO Beihua, HUANG Shengliang, et al. Establishment of HPLC fingerprint, cluster analysis and PLS-DA of Ligusticum chuanxiong decoction pieces[J]. China Pharmacy, 2019, 30(8): 1066-1071. DOI: 10.6039/j.issn.1001-0408.2019.08.11http://dx.doi.org/10.6039/j.issn.1001-0408.2019.08.11.
裴科, 宁燕, 蔡皓, 等. 基于HPLC指纹图谱结合化学模式识别的川芎炮制前后对比研究[J]. 中草药, 2021, 52(5): 1274-1283. DOI: 10.7501/j.issn.0253-2670.2021. 05.007http://dx.doi.org/10.7501/j.issn.0253-2670.2021.05.007.
PEI Ke, NING Yan, CAI Hao, et al. Comparative study of Chuanxiong rhizoma before and after processing based on HPLC fingerprints combined with chemical pattern recognition[J]. Chinese Traditional and Herbal Drugs, 2021, 52(5): 1274-1283. DOI: 10.7501/j.issn. 0253-2670.2021.05.007http://dx.doi.org/10.7501/j.issn.0253-2670.2021.05.007.
张艳欣, 吴佩颖, 王丹丹. 中药指纹图谱中常用的数理统计方法[J]. 上海医药, 2019, 40(9): 74-77. 10.3969/j.issn.1006-1533.2019.09.025http://dx.doi.org/10.3969/j.issn.1006-1533.2019.09.025
ZHANG Yanxin, WU Peiying, WANG Dandan. Mathematical statistical methods commonly used in fingerprints of TCM[J]. Shanghai Medical & Pharmaceutical Journal, 2019, 40(9): 74-77. 10.3969/j.issn.1006-1533.2019.09.025http://dx.doi.org/10.3969/j.issn.1006-1533.2019.09.025
王钢, 王丹, 何毅, 等. 麦冬、大黄饮片电子束辐照灭菌工艺研究[J]. 核农学报, 2022, 36(8): 1579-1588. DOI: 10.11869/j.issn.100-8551.2022.08.1579http://dx.doi.org/10.11869/j.issn.100-8551.2022.08.1579.
WANG Gang, WANG Dan, HE Yi, et al. Study on the decontamination process of decoction pieces of Ophiopogon japonicus and Rheum palmatum L. by electron beam irradiation[J]. Journal of Nuclear Agricultural Sciences, 2022, 36(8): 1579-1588. DOI: 10. 11869/j.issn.100-8551.2022.08.1579http://dx.doi.org/10.11869/j.issn.100-8551.2022.08.1579.
孔瑶, 姜鹏, 詹常森. 60Co-γ射线辐照灭菌对药用大黄粉指纹图谱及有效成分的影响[J]. 中国药师, 2022, 25(4): 702-708. DOI: 10.19962/j.cnki.issn1008-049X.2022.04.026http://dx.doi.org/10.19962/j.cnki.issn1008-049X.2022.04.026.
KONG Yao, JIANG Peng, ZHAN Changsen. Effects of 60Co-γ ray irradiation sterilization on the fingerprint and active ingredients of radixet rhizoma rhei powder[J]. China Pharmacist, 2022, 25(4): 702-708. DOI: 10.19962/j.cnki.issn1008-049X.2022.04.026http://dx.doi.org/10.19962/j.cnki.issn1008-049X.2022.04.026.
国家药典委员会. 中华人民共和国药典(2020年版). 四部[M]. 北京: 中国医药科技出版社, 2020: 205.
Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China 2020. Four parts[M]. Beijing: China Medical Science Press, 2020: 205.
胥双, 彭艳梅, 苏文俏, 等. 不同灭菌方法对天麻首乌片生药粉的灭菌效果及有效成分含量的影响[J]. 湖南中医杂志, 2019, 35(12): 115-116. DOI: 10.16808/j.cnki.issn1003-7705.2019.12.047http://dx.doi.org/10.16808/j.cnki.issn1003-7705.2019.12.047.
XU Shuang, PENG Yanmei, SU Wenqiao, et al. Sterilizing effect of different sterilizing methods on crude powder of Tianma Shouwu Tablets and their influence on the content of effective constituents[J]. Hunan Journal of Traditional Chinese Medicine, 2019, 35(12): 115-116. DOI: 10.16808/j.cnki.issn1003-7705.2019.12.047http://dx.doi.org/10.16808/j.cnki.issn1003-7705.2019.12.047.
蒋桃. 60Co-γ射线辐照灭菌对六味安消散中指纹图谱和有效成分含量的影响[J]. 药物分析杂志, 2019, 39(9): 1704-1712. DOI: 10.16155/j.0254-1793.2019.09.23http://dx.doi.org/10.16155/j.0254-1793.2019.09.23.
JIANG Tao. Influence of 60Co-γ radiation sterilization on the fingerprint and contents of effective constituents in Liuwei Anxiao powder[J]. Chinese Journal of Pharmaceutical Analysis, 2019, 39(9): 1704-1712. DOI: 10.16155/j.0254-1793.2019.09.23http://dx.doi.org/10.16155/j.0254-1793.2019.09.23.
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