辐射诱变贝莱斯芽孢杆菌及其对辣椒炭疽病抑菌效果

谢慧; 刘安; 陈亮; 武小芬; 齐慧; 张祺玲; 王振; 邓明; 王克勤; 刘素纯

doi:10.11889/j.1000-3436.2023-0029

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辐射诱变贝莱斯芽孢杆菌及其对辣椒炭疽病抑菌效果

辐射技术的应用 | 更新时间：2023-06-21

- 辐射诱变贝莱斯芽孢杆菌及其对辣椒炭疽病抑菌效果
  封面论文
- Radiation mutagenesis of Bacillus velezensis and its antibacterial effect on Colletotrichum gloeosporioides
- 辐射研究与辐射工艺学报 2023年41卷第3期页码：30401
- 作者机构：
  
  1.湖南农业大学食品科学技术学院长沙 410125
  2.湖南省农业科学院湖南省核农学与航天育种研究所长沙 410125
- 作者简介：
  
  谢慧，女，1998年1月出生，2020年于湖南农业大学东方科技学院获工学学士学位，现为湖南农业大学生物与医药专业硕士研究生
  王克勤，研究员，E-mail: wkq6412@163.com
  刘素纯，教授，E-mail: liusuchun@163.com
- 基金信息：
  
  国家重点研发计划项目(2021YFE0111200-2);湖南省农业科技创新项目(2022CX119)
- DOI：10.11889/j.1000-3436.2023-0029
  中图分类号：
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谢慧, 刘安, 陈亮, 等. 辐射诱变贝莱斯芽孢杆菌及其对辣椒炭疽病抑菌效果[J]. 辐射研究与辐射工艺学报, 2023,41(3):030401.

XIE Hui, LIU An, CHEN Liang, et al. Radiation mutagenesis of Bacillus velezensis and its antibacterial effect on Colletotrichum gloeosporioides[J]. Journal of Radiation Research and Radiation Processing, 2023,41(3):030401.
谢慧, 刘安, 陈亮, 等. 辐射诱变贝莱斯芽孢杆菌及其对辣椒炭疽病抑菌效果[J]. 辐射研究与辐射工艺学报, 2023,41(3):030401. DOI： 10.11889/j.1000-3436.2023-0029.

XIE Hui, LIU An, CHEN Liang, et al. Radiation mutagenesis of Bacillus velezensis and its antibacterial effect on Colletotrichum gloeosporioides[J]. Journal of Radiation Research and Radiation Processing, 2023,41(3):030401. DOI： 10.11889/j.1000-3436.2023-0029.

摘要

采用,60,Co-γ射线和电子束辐射诱变贝莱斯芽孢杆菌选育遗传稳定的突变菌株，用平板对峙法及四点法对突变菌株进行初筛和复筛，研究其对辣椒炭疽病菌的抑菌效果。结果表明：菌悬液经,60,Co-γ射线辐照，贝莱斯芽孢杆菌,D,10,值为2 366 Gy，在100~2 000 Gy诱变剂量范围内芽孢杆菌致死率随着辐照吸收剂量的增加而升高。在2 000 Gy辐照吸收剂量下，贝莱斯芽孢杆菌的致死率为81.8%，并筛选出5株（B004、B112、B114、B117、B118）突变菌株，其对辣椒炭疽病菌抑制率由出发菌株的51.2%分别提高到57.5%、58.0%、57.0%、57.7%和59.9%；贝莱斯芽孢杆菌菌悬液经电子束（10 MeV）辐照，贝莱斯芽孢杆菌,D,10,值为499 Gy，在100~600 Gy诱变剂量范围内，芽孢杆菌致死率随着辐照吸收剂量的增加而升高，在400 Gy辐照吸收剂量下，贝莱斯芽孢杆菌致死率为91.8%，筛选出2株（D115和D243）突变菌株，其对辣椒炭疽病菌抑制率由出发菌株的55.2%分别提高到58.4%和58.1%；B004、B112、B117、B118贝莱斯芽孢杆菌诱变菌株对辣椒炭疽病菌抑菌效果在6代内遗传稳定。研究结果表明：γ射线和电子束辐照在贝莱斯芽孢杆菌的诱变育种中具有潜在的应用价值，本研究可为贝莱斯芽孢杆菌的诱变育种提供基础，为辣椒炭疽病病害的生物防治提供理论参考。

Abstract

We used ,60,Co-γ ray and electron beam irradiation to mutate ,Bacillus velezensis, to breed genetically stable mutant strains. The mutant strains were screened and re-screened by plate confrontation and the four-point method to study their antibacterial effects on ,Colletotrichum gloeosporioides,. The results demonstrated that the ,D,10 ,value of ,Bacillus velezensis, suspension irradiated by ,60,Co-γ ray was 2 366 Gy, and the lethality rate increased with an increase in the irradiation coefficient dose in the range of 100-2 000 Gy. The lethality rate of ,Bacillus velezensis, was 81.8% at 2 000 Gy irradiation coefficient dose, and five mutants (B004, B112, B114, B117, and B118) were screened. The inhibition rate against ,Colletotrichum gloeosporioides, increased from 51.2% of the original strain to 57.5%, 58.0%, 57.0%, 57.7%, and 59.9%, respectively. The ,Bacillus velezensis, suspension was irradiated by an electron beam (10 MeV), the ,D,10, value was 499 Gy, and the lethality rate increased with an increase in the irradiation coefficient dose in the range of 100-600 Gy. The lethality rate of ,Bacillus velezensis, was 91.8% at 400 Gy irradiation coefficient dose. Two mutant strains (D115 and D243) were screened, and their inhibition rates against ,Colletotrichum gloeosporioides, increased from 55.2% of the emitted strains to 58.4 and 58.1%, respectively. The inhibitory effects of B004, B112, B117, and B118 mutants on ,Colletotrichum gloeosporioides, were genetically stable within six generations. In conclusion, γ-ray and electron beam irradiation have potential application value in the mutation breeding of ,Bacillus velezensis,. The results provide a basis for ,Bacillus velezensis, mutation breeding and a theoretical reference for the biological control of ,Colletotrichum gloeosporioides.

关键词

贝莱斯芽孢杆菌辣椒炭疽病菌60Co-γ射线辐射诱变电子束辐射诱变抑菌

Keywords

Bacillus velezensisColletotrichum gloeosporioides60Co-γ ray radiation mutagenesiselectron beam radiation mutagenesisBacteriostasis

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