To investigate the effect of artesunate on radio-sensitivity of human cervical cancer cells in vitro, The human cervical cancer cells HeLa and Siha were used as the experimental cells. MTT assay was used to determine the most appropriate drug concentration in the subsequent experiment, and the effect of human cervical cancer cells treated with artesunate and irradiation of ,60,Co γ-rays was studied by using conventional chromosomal aberration analysis and cytokinesis block method (CB method). The results show that when the concentration of artesunate in this experiment was 2.0 μmol/mL for HeLa cell and 4.0 μmol/mL for Siha cell respectively, the chromosome aberration, micronuclei cell and micronuclei rates of HeLa cells treated with artesunate were more serious than that of the only irradiation, but there is almost no change with Siha cells.