用人外周血线粒体DNA 4977bp缺失检测辐射损伤初探

封江彬; 陆雪; 陈德清; 陈晓穗; 刘青杰

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用人外周血线粒体DNA 4977bp缺失检测辐射损伤初探

研究论文 | 更新时间：2023-03-30

- 用人外周血线粒体DNA 4977bp缺失检测辐射损伤初探
- The primary study on radiation damage induced by ionizing radiation with human peripheral blood mitochondrial dna common deletion analysis
- 辐射研究与辐射工艺学报 2004年22卷第5期页码：311-314
- 作者机构：
  
  1.中国疾病预防控制中心辐射防护与核安全医学所　北京　100088
  2.海军总医院　北京　100037
- 作者简介：
  
  封江彬，女，1963年11月出生，1991年毕业于北京职工医学院医学检验专业，主管技师，放射生物学专业
- 基金信息：
  
  国家人事部留学回国人员择优资助项目（2002年优秀类）
- DOI：
  中图分类号：
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封江彬, 陆雪, 陈德清, 等. 用人外周血线粒体DNA 4977bp缺失检测辐射损伤初探[J]. 辐射研究与辐射工艺学报, 2004,22(5):311-314.

FENG Jiangbin, LU Xue, CHEN Deqing, et al. The primary study on radiation damage induced by ionizing radiation with human peripheral blood mitochondrial dna common deletion analysis[J]. Journal of Radiation Research and Radiation Processing, 2004,22(5):311-314.
封江彬, 陆雪, 陈德清, 等. 用人外周血线粒体DNA 4977bp缺失检测辐射损伤初探[J]. 辐射研究与辐射工艺学报, 2004,22(5):311-314. DOI：

FENG Jiangbin, LU Xue, CHEN Deqing, et al. The primary study on radiation damage induced by ionizing radiation with human peripheral blood mitochondrial dna common deletion analysis[J]. Journal of Radiation Research and Radiation Processing, 2004,22(5):311-314. DOI：

摘要

初步探索用聚合酶链反应（PCR）方法进行辐射诱导的线粒体DNA4977bp缺失分析的可行性。对正常人外周血进行2Gy ,60,Co γ射线的照射，照射后提取包含线粒体DNA的全基因组DNA，用PCR方法进行线粒体DNA 4977bp缺失分析，同时扩增很少缺失的线粒体DNA片段（16S−ND1）；进而比较照射前后线粒体DNA 4977bp缺失水平。结果表明，用于分析线粒体DNA 4977bp缺失的引物对在最佳试验条件下，可特异性扩增出所有2Gy照射诱导的线粒体DNA 4977bp缺失；该扩增产物经纯化、测序和核苷酸同源性分析，证实线粒体缺失发生在线粒体DNA序列的8470−13446bp。用于扩增16S−ND1的引物对在很大的温度变动范围内，均能扩出目的DNA片段。所有外周血样品在照射前无线粒体DNA 4977bp缺失，2Gy ,60,Coγ射线照射后特异诱导出此缺失。说明用本研究中建立的PCR方法，可用来分析电离辐射诱导的外周血有核细胞线粒体DNA 4977bp缺失水平。

Abstract

This study is try to explore if the polymerase chain reaction (PCR) could be used to analyze the mitochondrial DNA 4977bp deletion (common deletion) induced by ionizing radiation. Peripheral blood samples were exposed to 2Gy ,60,Co γ-rays in vitro, and genomic DNA, including mitochondrial DNA, was extracted. A PCR method was tried to analyze the common deletion, including seldom deleted 16S-ND1 gene. The common deletions before and after the 2Gy exposure were analyzed with the PCR method. The results showed that the common deletion PCR products were detected after exposure to 2Gy in each peripheral blood sample. The purified PCR products were sequenced and carried out BLAST on standard human mitochondrial genome sequence database. The result showed that the common deletion flanked between 8470bp and 13446bp. Before exposed to ,60,Co γ-rays, no common deletion were not detected in the samples.These observations suggest that the PCR method, established in this study, could be used to detect the common deletion induced by ionizing radiation on peripheral lymphocytes samples.

关键词

60Co γ射线线粒体DNA常见缺失PCR扩增

Keywords

60Co γ-raysMitochondrial DNA common deletionPCR amplification

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