FANG Chuanji, LU Zhaoxin, SUN Lijun, et al. Study on mutation breeding and fermentation of antimicrobial lipopeptides yielding bacterium with 20keV N+ ion beam implantation[J]. Journal of Radiation Research and Radiation Processing, 2006,24(6):333-336.
FANG Chuanji, LU Zhaoxin, SUN Lijun, et al. Study on mutation breeding and fermentation of antimicrobial lipopeptides yielding bacterium with 20keV N+ ion beam implantation[J]. Journal of Radiation Research and Radiation Processing, 2006,24(6):333-336.DOI:
Bacillus amyloliquefaciens, ES-2 was implanted with 20keV N,+, ion beam to breed mutants of high yield of antimicrobial lipopeptides. The dose effects of the nitrogen ion implantation on the survival and positive mutations rate was studied. The mutant strain designated as ,B. amyloliquefaciens, ES-2-4 showing higher yield of antimicrobial lipopeptides was obtained. The concentration of the lipopeptides in fermentation liquid increased by 15.2% compared with the original strain. We also studied the fermentation process between the mutant and the original strain. It indicated that the mutant with shorter lag phase, longer stable phase and higher yield of the lipopeptides, which was suitable for large-scale production.
关键词
离子注入脂肽诱变选育发酵
Keywords
Ion implantationAntimicrobial lipopeptidesMutation breedingFermentation