A hydroxyl methacrylate monomer,o,-hydroxycyclohexyl methacrylate (HCMA), was synthesized by opening ring reaction of cyclohexene oxide with methacrylic acid. Real time infrared spectroscopy (RTIR) was used to study the effect of molecule structure, light intensity, various photo-initiator and initiator concentration on photo-polymerization kinetics. HCMA and HEMA as comonomers were utilized to prepare hydrogel by photo-polymerization method. The mechanical strength of poly (hydroxyethyl methacrylate) (HEMA) and poly (HEMA-co-HCMA) hydrogel was conducted by dynamic mechanical analysis (DMA). The results demonstrated that increasing of light intensity and photo-initiator concentration escalate final double bond conversion. And the storage module and loss module of poly (HEMA-co-HCMA) hydrogel were higher than those of poly (HEMA) hydrogel.