On the basis of practical application combined with current domestic study, to provide evidences for the selection of appropriate dose setting method. In our test 148 samples collected were from 52 manufacturers all over China. After bioburden estimate, the dose setting was performed with ISO11137 method 1 along with other two method (Ig method and VD,max(-1), method). The bioburden on product ranges from 7.17 to 1.98×10,5,cfu/unit. On the same level of bioburden, the sterilization dose from ISOl1137 method 1 is more than that from Ig method. Both of them are less than that from VD,max, (-1). It is thought that the VD,max,(-1) method is perfect for product with less contamination, ISO11137 method 1 is perfect for produced regularly. The dose safety of Ig method which used in china is suspicious.