S-adenosyl-L-methionine (SAM) and glutathione (GSH) are both applied extensively in the fields of medicine, cosmetics and health maintenance for their biological functions multiplicity in vivo. In this paper, a complex mutagenesis was carried out to attenuate the contradiction so as to obtain a positive mutant which can produce SAM and GSH effectively. The parent strain ,Candida utilis, SZU 07-01 was treated by two turns of UV and once γ-rays irradiation for complex mutagenesis, following by cultivating and screening on the selective medium (YPD containing 1 g/L L-Met, 0.5 g/L ethionine and 2 g/L NaHSO,3,) for a positive mutant ,C. utilis, SUGS-01. The proliferation of ,C. utilis, SUGS-01 was then carried out in a flask, and the productions of S-adenosyl-L-methionine and glutathione were 324.0 mg/L and 220.5 mg/L, respectively, which were 131.4% and 26.0% higher than those of ,C. utilis, SZU 07-01. In addition,C. utilis, SUGS-01 shows high genetic stability even after 10 generations of cultivation, which indicates that the mutant ,C. utilis, SUGS-01 obtained in this study has stable capacity for high co-production of SAM and GSH, and can be used as industrial strain in the near future.