小鼠扁桃体免疫细胞受照射后DNA双链断裂损伤修复的忠实性

吴玮; 屈昌民; 吴代民

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小鼠扁桃体免疫细胞受照射后DNA双链断裂损伤修复的忠实性

研究论文 | 更新时间：2023-04-07

- 小鼠扁桃体免疫细胞受照射后DNA双链断裂损伤修复的忠实性
- THE EFFICIENCY AND FIDELITY OF REJOINING OF THE DNA DOUBLE STRAND BREAK IN THE IMMUNOCYTES OF TONSILS OF IRRADIATED MICE
- 辐射研究与辐射工艺学报 2002年20卷第2期页码：146-150
- 作者机构：
  
  1.中国人民解放军第三0六医院　北京　100101
- 作者简介：
  
  吴玮，女，1963年8月出生，1998年毕业于第二军医大学博士，临床免疫专业，副主任医师
- 基金信息：
  
  总装备部医药卫生重点科研课题基金(299004)
- DOI：
  中图分类号：
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吴玮, 屈昌民, 吴代民. 小鼠扁桃体免疫细胞受照射后DNA双链断裂损伤修复的忠实性[J]. 辐射研究与辐射工艺学报, 2002,20(2):146-150.

WU Wei, QU Changmin, WU Daimin. THE EFFICIENCY AND FIDELITY OF REJOINING OF THE DNA DOUBLE STRAND BREAK IN THE IMMUNOCYTES OF TONSILS OF IRRADIATED MICE[J]. Journal of Radiation Research and Radiation Processing, 2002,20(2):146-150.
吴玮, 屈昌民, 吴代民. 小鼠扁桃体免疫细胞受照射后DNA双链断裂损伤修复的忠实性[J]. 辐射研究与辐射工艺学报, 2002,20(2):146-150. DOI：

WU Wei, QU Changmin, WU Daimin. THE EFFICIENCY AND FIDELITY OF REJOINING OF THE DNA DOUBLE STRAND BREAK IN THE IMMUNOCYTES OF TONSILS OF IRRADIATED MICE[J]. Journal of Radiation Research and Radiation Processing, 2002,20(2):146-150. DOI：

摘要

观察了受照射小鼠瓦尔代尔扁桃体环（WRE）在体细胞出现DNA双链断裂修复的忠实性，用,60,Co ,γ,射线照射后观察WRE细胞凋亡的最高峰，在此时间点处死小鼠，取WRE细胞分别用脂质体和电穿孔方法介导的基因转染技术，将双标记基因质粒pPMH16导入细胞。研究WRE细胞DNA双链修复的忠实性。结果表明，未照射WRE细胞的抗G418转化克隆有69.75%的gpt基因表达，说明大部分断裂的gpt基因被忠实性地修复；2Gy、4Gy射线照射的WRE细胞的转化克隆只有36.05%和21.50%的克隆能正确表达gpt基因功能，说明WRE细胞照射后DNA双链断裂的修复显著低于正常细胞，且剂量越大，修复的忠实性越低。脂质体和电穿孔方法介导的基因转染技术可用于原代细胞基因转染的研究，照射后的WRE细胞易发生DNA双链断裂错误重接。

Abstract

To examine the efficiency and fidelity of rejoining of the double strand break(dsb) DNA of the irradiated mouse Waldeyeer s Ring Equivalent (WRE) cells. With lipofectamine or electroporation, the linearized plasmid pPMH16 with two marker genes (neo and gpt)were transferred into the WRE cells from irradiated mice. Through ordinal G418 screen and XHAYM screen, the rejoining efficiency of the broken gpt gene wes evaluated. The rejoining efficiency of gpt gene was 69.75% for control WRE cells, and 36.05% for the WRE cells gotten form the irradiated mice 4h after exposure to 2Gy γ irradiated and 21.50% 4h after exposure to 4Gy γ irradiated. The results showed that the fidelity of the rejoining of dsb of DNA for the irratiated mouse WRE cells was lower than that for control, and as the radiation dose increased,the fidelity decreased. Both the lipofectamine or electroporation could be successfully used in gene transfection for primary culture of mouse WRE cells. And the wrong repair of dsb of DNA in irradiated mouse WRE cells might be caused frequently.

关键词

DNA修复忠实性基因转染WRE细胞辐射损伤

Keywords

Fidelity of DNA repairGene transferredWaldeyer's—Ring Equivalent cellsRadiation injury

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