过热对细胞DNA辐射损伤与修复的影响Ⅰ滤膜—荧光法及其在DNA结构检测中的应用

孟祥顺; 郑秀龙

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过热对细胞DNA辐射损伤与修复的影响Ⅰ滤膜—荧光法及其在DNA结构检测中的应用

研究论文与科研成果报告 | 更新时间：2023-04-23

- 过热对细胞DNA辐射损伤与修复的影响Ⅰ滤膜—荧光法及其在DNA结构检测中的应用
- THE EFFECTS OF HYPERTHERMIA ON RADIATION-INDUCED DNA DAMAGE AND ITS REPAIR ⅠThe Method of Alkaline Elution-Fluorometric Assay and Its Application on Studying DNA Structure
- 辐射研究与辐射工艺学报 1984年2卷第3期页码：25-31
- 作者机构：
  
  1.上海第二军医大学
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孟祥顺, 郑秀龙. 过热对细胞DNA辐射损伤与修复的影响Ⅰ滤膜—荧光法及其在DNA结构检测中的应用[J]. 辐射研究与辐射工艺学报, 1984,2(3):25-31.

Meng Xiangshun, Zheng Xiulong. THE EFFECTS OF HYPERTHERMIA ON RADIATION-INDUCED DNA DAMAGE AND ITS REPAIR ⅠThe Method of Alkaline Elution-Fluorometric Assay and Its Application on Studying DNA Structure[J]. Journal of Radiation Research and Radiation Processing, 1984,2(3):25-31.
孟祥顺, 郑秀龙. 过热对细胞DNA辐射损伤与修复的影响Ⅰ滤膜—荧光法及其在DNA结构检测中的应用[J]. 辐射研究与辐射工艺学报, 1984,2(3):25-31. DOI：

Meng Xiangshun, Zheng Xiulong. THE EFFECTS OF HYPERTHERMIA ON RADIATION-INDUCED DNA DAMAGE AND ITS REPAIR ⅠThe Method of Alkaline Elution-Fluorometric Assay and Its Application on Studying DNA Structure[J]. Journal of Radiation Research and Radiation Processing, 1984,2(3):25-31. DOI：

摘要

本文报道了用输液夹代替蠕动泵调控洗脱速度，改进和建立了能用于分裂和不分裂细胞DNA单链断裂检测的微孔滤膜硷洗脱—荧光法。对其中一些影响因素作了较详细的探讨。结果表明：改进后的方法灵敏、简便、重复性较好，可进行批量样品分析。并用此法研究了小鼠淋巴细胞型白血病L,7712,细胞DNA辐射损伤与修复的规律。

Abstract

In this paper we describe an improved alkaline filter elution-fluorometric method for detection cell DNA radiation damage and its repair. We used screw clamp in place of peristaltic pump to adjust elution rate acquired the same results. The influence of some factors on this method was also studied.,Immediately after r-ray irradiation (5~30 Gy) of L,7712, cells, it was shown a significant nagative linear relationship between doses and the percentage of DNA remaining on the filter. The repair of damaged DNA could also be measured by our method. This improved method had good reproducibility, sensitivity and could proceed in batch analysis of samples.

关键词

微孔滤膜硷洗脱荧光法检测35—二氨基苯甲酸盐酸盐L7712细胞DNA单链断裂与重接

Keywords

Millipore filter alkaline elutionFluorometric assay35-Diaminobenzoic acid dihydrochlorideL7712 cellsDNA single strand breaks and repair

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