用UPG染色方法分析低剂量<sup>60</sup>Coγ线诱发离体人外周血淋巴细胞染色体畸变

张鸿源; 潘豫沙; 王兰金

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用UPG染色方法分析低剂量⁶⁰Coγ线诱发离体人外周血淋巴细胞染色体畸变

科研简报 | 更新时间：2023-04-23

- 用UPG染色方法分析低剂量⁶⁰Coγ线诱发离体人外周血淋巴细胞染色体畸变
- APPLICATION OF UPG STAINING FOR ANALYSIS OF CHROMOSOME ABERRATIONS INDUCED BY LOW-DOSE ⁶⁰Co-γ-RAYS IN HUMAN PERIPHERAL BLOOD LYMPHOCYTES IN VITRO
- 辐射研究与辐射工艺学报 1988年6卷第3期页码：44-47
- 作者机构：
  
  1.海军医学研究所，上海
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张鸿源, 潘豫沙, 王兰金. 用UPG染色方法分析低剂量⁶⁰Coγ线诱发离体人外周血淋巴细胞染色体畸变[J]. 辐射研究与辐射工艺学报, 1988,6(3):44-47.

Zhang Hongyuan, Pan Yusha, Wan Lanjin. APPLICATION OF UPG STAINING FOR ANALYSIS OF CHROMOSOME ABERRATIONS INDUCED BY LOW-DOSE ⁶⁰Co-γ-RAYS IN HUMAN PERIPHERAL BLOOD LYMPHOCYTES IN VITRO[J]. Journal of Radiation Research and Radiation Processing, 1988,6(3):44-47.
张鸿源, 潘豫沙, 王兰金. 用UPG染色方法分析低剂量⁶⁰Coγ线诱发离体人外周血淋巴细胞染色体畸变[J]. 辐射研究与辐射工艺学报, 1988,6(3):44-47. DOI：

Zhang Hongyuan, Pan Yusha, Wan Lanjin. APPLICATION OF UPG STAINING FOR ANALYSIS OF CHROMOSOME ABERRATIONS INDUCED BY LOW-DOSE ⁶⁰Co-γ-RAYS IN HUMAN PERIPHERAL BLOOD LYMPHOCYTES IN VITRO[J]. Journal of Radiation Research and Radiation Processing, 1988,6(3):44-47. DOI：

摘要

本研究用0.0498—0.5240 Gy ,60,Co-γ-线照射离体人外周血淋巴细胞，用UPG染色技术分析染色体畸变的剂量效应关系。结果表明：只分析M,1,细胞的染色体畸变比常规方法较切实地反映畸变的计数率；在所照的剂量范围内，双着丝粒体加着丝粒环可较好地拟合线性或线性二次方程，无着丝粒断片可较好地拟合线性方程。最后，就低剂量辐射后用双着丝粒体加着丝粒环和无着丝粒断片作细胞遗传剂量测定的问题进行了讨论。

Abstract

Human peripheral blood lymphocytes in vitro were irradiated by ,60,Co-,γ, -rays with 0.0489-0.524 Gy. By using UPG staining technique, the dose response relationships of chromosome aberrations were studied. The analysis of exclusively M,1, cells, revealed surely aberration scored yields as compared with the results of the conventional method. The data of dicentrics plus centric rings of M,1, cells give a good fit to linear or linearquadratic model in this dose range, and the data of acentics give a good fit to linear model. Finally, the results were discussed with regard to the application of dicentrics olus centric rings and acentrics for a cytogenetic dosimetry after exposure to low levels of ionizing radiation.

关键词

低剂量染色体畸变无着丝粒断片双着丝粒体剂量—效应曲线UPG

Keywords

Low-doseChromosome aberrationAcentric fragmentDicentricsDose-response curveUPG

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APPLICATION OF UPG STAINING FOR ANALYSIS OF CHROMOSOME ABERRATIONS INDUCED BY LOW-DOSE 60Co-γ-RAYS IN HUMAN PERIPHERAL BLOOD LYMPHOCYTES IN VITRO

DOI：

摘要

Abstract

关键词

Keywords

references

用UPG染色方法分析低剂量⁶⁰Coγ线诱发离体人外周血淋巴细胞染色体畸变

APPLICATION OF UPG STAINING FOR ANALYSIS OF CHROMOSOME ABERRATIONS INDUCED BY LOW-DOSE ⁶⁰Co-γ-RAYS IN HUMAN PERIPHERAL BLOOD LYMPHOCYTES IN VITRO