The rape pollen contains many biologically active and nutritive substances that are easily spoilt by bacteria because there are abundant nutritive substances. In the present study on disinfection, the rape pollen was irradiated with 2.5 kGy ,60,Coγ-ray. We used micronuciei, sister chromatid exchanges (SCE) of bone marrow cells and chromoscmal aterrations of meiotic cells in mice as an indicater of chromosomal damage to study the mutagenicity of irradiated rape pollen. The results are as follows:,(1). Micronuclei: The frequency of micronuclei in polychromatic erythrocytes is 2.00‰; nucleated cells is 0.85‰ in control group. In the numbers of polychromatic erythrocytes and nucleated cells with micronuclei, there is no obviously difference in irradiated and unirradiated groups.,(2) SCE: SCE incidence of control group is 2.01±0.12 /cell. No significant difference in the frequency of SCE exists between non irradited rape pollen and the control groups. But the frequency of SCE in irradiated rape pollen group (3000 mg/kg/day×7) is 2.36±0.12/cell; high dose group (6000 mg/kg/day×7) is 2.96± 0.14/cell. In comparison with control group, there is significant difference.,(3). The meiotic chromosomal aberration: The chromatid breaks, fragments, and univalents in primary spermatocytes have been obseved. The frequencies of chromosomal aberration showed no obviously difference among irradiated and non irradiated rape pollen groups. The univalent frequencies of X-Y chromosomes in the experimental groups didn't found the difference with the control group which is 9.2% and ovre similar to that of the reference.