
1.苏州医学院,苏州
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朱寿彭, 汪源长. 核素147Pm在细胞内滞留的电镜放射自显影研究[J]. 辐射研究与辐射工艺学报, 1992,10(1):12-19.
Zhu Shoupeng, Wang Yuanchang. ACCUMULATION OF RADIONUCLIDE 147Pm IN SUBCELLULAR LEVEL BY ELECTRON MICROSCOPIC AUTORADIOGRAPHY[J]. Journal of Radiation Research and Radiation Processing, 1992,10(1):12-19.
本文用电镜放射自显影术研究了重核裂片,147,Pm在体内亚细胞水平滞留动态。发现,147,Pm的内污染沉积早期在血细胞和血管内皮细胞中,尤以在红细胞的沉积为多。,147,Pm呈选择性滞留于肝细胞亚微结构中,可较多的掺入枯否细胞、肝细胞核和核仁内,在胞质中的糙面内质网、线粒体、微体和糖元中亦有滞留,且主要沉积于糙面内质网和线粒体,在高尔基复合体与糙面内质网所芽生的转移小泡融合处,亦有,147,Pm滞留的放射自显影象。而在肾小体足细胞的线粒体和溶酶体中,有浓集的放射自显影径迹呈现。在肾近端小管曲部上皮细胞核中和胞质的线粒体和微体中,亦有较多的,147,Pm滞留。,随着观察时间的延续,,147,Pm在骨有机质亚微结构中呈现选择性、持续性的沉积增多。可浓集于破骨细胞和成骨细胞核中。此外在骨组织细胞间质中的胶原纤维部位,亦有较多的,147,Pm沉积,难以排除。
This study investigated the subcellular localization of radionuclide ,147,Pm in tissue cells by electron microscopic autoradiography. The early risk of internal contaminated accumulation of ,147,Pm was in blood cells and endothelial cells, especially in red blood cells where more numerous of autoradiographic tracks appeared. Then ,147,Pm was selectively deposited in ultrastructure of liver cells, such as in nucleus, nucleous, rough endoplasmic reticulum, mitochondria and microbodies. In organelleg ,147,Pm was mainly accumulated in rough endoplasmic reticulum and mitochondria. Autoradiographic tracks especially localized in combined point between Golgi complex and transitive vesicle of rough endoplasmic reticulum. Autoradiographic study also demonstrated, where dense tracks appeared in mitochondria and lysosome of podal cells within renal corpuscle. In nucleus as well as in mitochondria and microbodies within epicyte of kidney near-convoluted tubule where also more numerous tracks of ,147,Pm accumulated. With the prolongation of observing time,147,Pm was selectively and steadily deposited in subcellular level of organic component bone. Electron microscopic autoradiography showed, that substential amounts of ,147,Pm was taken up into the nuclear fraction of osteoclasts and osteoblasts. In addition, where more numerous tracks of ,147,Pm was deposited in collagenous fibre within interstitial of bone cells and was hardly excreted.
电镜放射自显影术滞留147Pm亚细胞水平
Electron microscopic autoradiographyAccumulation147PmSubcellular level
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