N<sub>2</sub>O饱和水溶液体系中胸腺嘧啶的辐射交联

赵蓉; 林念芸; 张加山

您当前的位置：

首页 >

文章列表页 >

N₂O饱和水溶液体系中胸腺嘧啶的辐射交联

更新时间：2024-11-05

- N₂O饱和水溶液体系中胸腺嘧啶的辐射交联
- γ-IRRADIATED CROSSLINKING OF THYMINE IN N₂O SATURATED AQUEOUS SOLUTION
- 辐射研究与辐射工艺学报 1990年8卷第1期页码：48-53
- 作者机构：
  
  中国科学院上海原子核研究所，上海
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助项目
- DOI：
  中图分类号：
- 纸质出版日期：1990-02，
  
  收稿日期：1989-03-06，
- 稿件说明：
移动端阅览
赵蓉, 林念芸, 张加山. N₂O饱和水溶液体系中胸腺嘧啶的辐射交联[J]. 辐射研究与辐射工艺学报, 1990,8(1):48-53.

Zhao Rong, Lin Nianyun, Zhang Jiashan. γ-IRRADIATED CROSSLINKING OF THYMINE IN N₂O SATURATED AQUEOUS SOLUTION. [J]. Journal of Radiation Research and Radiation Processing, 1990,8(1):48-53.
赵蓉, 林念芸, 张加山. N₂O饱和水溶液体系中胸腺嘧啶的辐射交联[J]. 辐射研究与辐射工艺学报, 1990,8(1):48-53. DOI：

Zhao Rong, Lin Nianyun, Zhang Jiashan. γ-IRRADIATED CROSSLINKING OF THYMINE IN N₂O SATURATED AQUEOUS SOLUTION. [J]. Journal of Radiation Research and Radiation Processing, 1990,8(1):48-53. DOI：

摘要

本文研究了在N

O饱和水溶液体系中，胸腺嘧啶的γ-辐解产物。用纸层析放射性分析法，测得胸腺嘧啶交联二聚体的产额G=2.6。用高效液相色谱放射性检测法和色谱质谱联用法，检测和鉴定胸腺嘧啶交联二聚体的结构，确定胸腺嘧啶交联二聚体是以嘧啶环上C（5）-C（5）单键连接的化合物，它们的进一步反应，形成各种不同结构的交联产物。

Abstract

In this work radioactive paper chromatograph was used to separate γ-irradiated products of 2-

C labelled thymine in N

O saturated solution and the G value of thymine dimers was determined to be 2.6. The preparative paper chromatography was adoped to isolate the dimers preliminary and then a high performance liquid chromatography equiped with a radiocativity detector was applied to separate both the preliminary isolated dimers and untreated sample. For further structure characterization of dimers a capillary gas chromatography-mass spectrometry (GC-MS)was used and the mass spectra were taken from each GC separated product peak of

-irradiated thymine in N

O saturated solution after trimethylsilylation by BSTFA. TMS derivatives of radiation induced dimers of thymine showed the molecular ion(M

) and a characteristic ion (M- CH

)

at 718

703; 628

613; 538

523; 540

525; These peaks were assigned to the following structures from several kinds of radical combination

such as

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=68635474&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=68635467&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=68635484&type=

The dehydration of this dimer derives a variety of crosslinked products:

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=68635487&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=68635476&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=68635479&type=

TMS derivatives

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=68635481&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=68635480&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=68635482&type=

Another kind of crosslinking is

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=68635499&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=68635498&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=68635500&type=

关键词

胸腺嘧啶辐射交联GC-MSHPLC

Keywords

ThymineRadiation CrosslinkingGC-MSHPLC

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

¹⁸O⁸⁺离子辐照胸腺嘧啶N₂O饱和水溶液的研究

辐射法制备聚乙烯醇（PVA）改性的聚对苯二甲酸乙二醇酯（PET）薄膜及其防雾性能

吸收剂量对二聚酸型聚酰胺结构及性能的影响

高强度氧化石墨烯/高取代羟丙基纤维素/聚乙烯醇复合水凝胶的辐射制备及其机械性能

辐照乙烯-四氟乙烯共聚物的热老化性能

N2O饱和水溶液体系中胸腺嘧啶的辐射交联

γ-IRRADIATED CROSSLINKING OF THYMINE IN N2O SATURATED AQUEOUS SOLUTION

DOI：

摘要

Abstract

关键词

Keywords

references

N₂O饱和水溶液体系中胸腺嘧啶的辐射交联

γ-IRRADIATED CROSSLINKING OF THYMINE IN N₂O SATURATED AQUEOUS SOLUTION