12C6+ ion beam was used to irradiate glutamic acid-producing strains, and the strains producing higher glutamate were screened by three selective breeding mediums, and then the mutants were fermented by shake flask experiments. Finally the mutants were measured by several fermentation experiments. The results showed that the survival rate of corynebacterium glutamicum changed significantly after irradiation with 80 MeV/u 12C6+ ion beam, and a high-yield strain GM006 was finally obtained through the two screening steps of selecting plate and shaking flask fermentation. The growth rate and glutamic acid yielding of GM006 were remarkably improved. The conversion rate of glucose was 60.55%, which increased by 10.09% compared with the original strain. The highest content of glutamic acid reached 121.10 g/L, and increased by 10.09 % compared with the original strain. This mutant will have broad application prospect as a glutamic acid producing strain, and it is also proved that the heavy ions beam is an efficient radiation resource for microorganism breeding.Cited