We established a chemiluminescent system for determination of lipid peroxidation and screening antioxidants. The lipid containing unsaturated fatly acids was injected into a glass tube. Luminol solution and the deionized water were added into it too. The glass tube was put into a preincubation box to incubate it for 0.5h at 37℃. AAPH solution was injected into the tube for immediate measurement in a biochimiluminometer at 38—39℃. The pulses / 6s (CP6s) were determined with T-2 program. Chemiluminescent dynamic and lipid peroxidation changes were observed continuously. Once the CL intensity of lipid peroxidation got peak, the antioxidant which has different concentration was added immediately in situ. A certain CL intensity (CP6s) was chosen as evaluation index to compare the activity of antioxidants. A luminol chemiluminescent system for determination of lipid peroxidation has been made. It was found that Vit.C, teapolyphenol, and glutathione have effects on scavenging lipid free radicals. The new method is quick, sensitive, and simple for determination of lipid peroxidation and screening antioxidants.