·OH reacts with carbonate to yield carbonate radical anion (CO,3,·,−,) and chemiluminescence. Based on this reaction, chemiluminescence behavior of the system was investigated. Experimental conditions for the method were optimized. A chemiluminescence system for determination of CO,3,·,−, was developed. It was composed of 2mmol/L CuCl solution 80μL, 6.5mmol/L H,2,O,2, solution 110μL and 75mmol/L carbonate buffer 810μL (pH9.8). The volume of reaction was 1.0mL. This system was characterized by its simplicity, rapidity, sensitivity and inexpensiveness. The recommended method had been successfully applied to determination of the ability of polyphenol, rutin, and quercetin scavenging CO,3,·,−, with satisfactory results.