In this paper the cellular uptake of sulphonated hypocrellin(S-HB), as well as photodamage on cellular viability,lipid peroxidation and intrinsic fluorescence quenching of membrane protein was studied.,It was found that S-HB suitable dissolved in aqueous solution, its cellular uptake is slower that HB. The photodamage on cellular viability both photosensitizers was close to each other, however the photosensitizers were different in physical and chemical properties. The HB photodamage target of cells was membrane, but the sulphonated HB photodamage target of cells may be part of organelles, besides the membrane. The experiments showed the sulphonated HB would be suggested as a potential advantage for photodynamic therapy of tumor in clinical application.