以PCC技术分析染色体辐射损伤的修复

杨新海; 饶用清

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以PCC技术分析染色体辐射损伤的修复

研究论文 | 更新时间：2023-04-26

- 以PCC技术分析染色体辐射损伤的修复
- ANALYSIS OF THE CHROMOSOME DAMAGE AND REPAIR KINETICS IN CHL CELLS AFTER ⁶⁰Co γ-IRRADIATION BY PCC TECHNIQUE
- 辐射研究与辐射工艺学报 1996年14卷第2期页码：104-106
- 作者机构：
  
  1.北京医科大学放射医学基础教研室，北京 100083
- 作者简介：
- 基金信息：
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杨新海, 饶用清. 以PCC技术分析染色体辐射损伤的修复[J]. 辐射研究与辐射工艺学报, 1996,14(2):104-106.

Yang Xinhai, Rao Yongqing. ANALYSIS OF THE CHROMOSOME DAMAGE AND REPAIR KINETICS IN CHL CELLS AFTER ⁶⁰Co γ-IRRADIATION BY PCC TECHNIQUE[J]. Journal of Radiation Research and Radiation Processing, 1996,14(2):104-106.
杨新海, 饶用清. 以PCC技术分析染色体辐射损伤的修复[J]. 辐射研究与辐射工艺学报, 1996,14(2):104-106. DOI：

Yang Xinhai, Rao Yongqing. ANALYSIS OF THE CHROMOSOME DAMAGE AND REPAIR KINETICS IN CHL CELLS AFTER ⁶⁰Co γ-IRRADIATION BY PCC TECHNIQUE[J]. Journal of Radiation Research and Radiation Processing, 1996,14(2):104-106. DOI：

摘要

采用PCC技术，观察CHL（Chinese hamster fibroblast cell line）细胞受,60,Co ,γ,射线照射后，染色体的损伤及修复动力学。结果表明，染色体损伤的修复分为两个时期，快修复在照射后的30 min左右开始，半修复时间约为6 h。与FADU（Fluorometric analysis of DNA unwinding）技术观察DNA损伤修复动力学比较，DNA损伤修复在照射后5 min内即已开始，70%以上的DNA损伤修复发生在15 min内。由两者损伤修复动力学不同推测两者修复机制有区别。

Abstract

The kinetics of chromosome damage and repair was determined by PCC. Chromosome damage repair was not detectable until 30 min after irradiation and nearly half of chromosome damage was repaired by 6 h. In contrast, significant DNA repair occured at 5 min after γ-irradiation measured by FADU technique. These results suggest that the early repairing DNA SSBs are not important in the formation of chromosome aberration, the different repair kinetics between DNA and chromosome damages might reflect their different repair mechanism.

关键词

PCC技术FADU技术DNA损伤和修复染色体损伤及修复

Keywords

Chromosome darage and repairPCC and FADUDNA damage and repair

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