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- Journal of Radiation Research and Radiation Processing Vol. 31, Issue 5, Pages: 6-030205(2013)
- 作者机构：
  
  1. 1（中国医学科学院放射医学研究所天津市分子核医学重点实验室）,天津,300192
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FU Yue1, WANG Yan1, DU Liqing1, et al. [J]. Journal of Radiation Research and Radiation Processing 31(5):6-030205(2013)
DOI：

FU Yue1, WANG Yan1, DU Liqing1, et al. [J]. Journal of Radiation Research and Radiation Processing 31(5):6-030205(2013) DOI：

摘要

研究Sirt1蛋白对间充质干细胞（MSCs）受照射后产生的炎症因子IL-1β的影响，探讨Sirt1蛋白在辐射防护中的作用。shRNA体外沉默间充质干细胞Sirt1基因转录，检测细胞内Sirt1mRNA表达证实基因沉默效果；同时给予间充质干细胞200 µmol•L−1白藜芦醇，经4 Gy照射后，用ELISA、Western blot和 RT-PCR等方法检测Sirt1沉默对IL-1β分泌和表达的影响。结果表明，照射前沉默Sirt1基因并给予间充质干细胞200 µmol•L−1白藜芦醇，与照射前单纯给予200 µmol•L−1白藜芦醇相比，辐射引起的IL-1β分泌显著升高(t=18.57, p<0.05)，同时细胞内IL-1β蛋白表达和mRNA水平也显著升高（t=8.078, p<0.05）。沉默Sirt1后可明显抑制白藜芦醇的辐射保护作用，提示Sirt1蛋白在辐射后产生炎症的过程中起到关键的调节作用。

Abstract

This paper investigated the effect of Sirt1 on inflammatory factor IL-1β produced in irradiated m esenchymal stem cells (MSCs) and explored the role of Sirt1 in radiation protection. shRNA silenced Sirt1 gene transcription in MSCs, and the intracellular Sirt1mRNA expression were detected to confirm the gene silencing, MSCs were treated with 200 μmol•L−1 resveratrol before irradiation, and the expression of IL-1β was detected by ELISA, western blotting and real time PCR after irradiation. The results show that the levels of IL-1β secretion increase compared with those of the resveratrol group (t=18.57, p<0.05). And meanwhile, the intracellular expression of IL-1β protein and mRNA of RNAi, combined with resveratrol group, increase significantly compared with those of the resveratrol group (t=8.078, p<0.05). Sirt1 gene silence can obviously inhibit the radiation protective effect of resveratrol, which suggests that Sirt1 may play a key role in the process of inflammation induced by irradiation.Cited

关键词

Sirt1蛋白间充质干细胞辐射防护白介素-1&beta

Keywords

Sirt1 proteinMesenchymal stem cellsRadiation protectionIL-1β

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