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- Journal of Radiation Research and Radiation Processing Vol. 31, Issue 4, Pages: 8-040207(2013)
- 作者机构：
  
  1. (苏州大学医学部放射医学与防护学院江苏省放射医学与防护重点实验室 ),苏州,215123
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LI Dongmei, HONG Chengjiao, ZHANG Baoguo. [J]. Journal of Radiation Research and Radiation Processing 31(4):8-040207(2013)
DOI：

LI Dongmei, HONG Chengjiao, ZHANG Baoguo. [J]. Journal of Radiation Research and Radiation Processing 31(4):8-040207(2013) DOI：

摘要

以宫颈癌细胞株HeLa为研究对象，采用CCK-8法检测AS1411对HeLa细胞生长的增殖-毒性作用，并利用克隆形成实验观察AS1411对Hela细胞放射敏感性的影响。结果表明：AS1411的细胞毒性很小，不同浓度（0 nmol/L、50 nmol/L、100 nmol/L、250 nmol/L、500 nmol/L）AS1411在24 h、48 h、72 h时间点的HeLa细胞存活率均>95%；克隆形成实验显示AS1411联合X射线能够降低照射后HeLa细胞的存活率，且HeLa细胞在药物浓度为100 nmol/L、250 nmol/L、500 nmol/L时的放射增敏比（SER）分别为1.06、1.58和1.89。本实验研究证实AS1411对HeLa细胞具有放射增敏作用。

Abstract

To investigate the effects of AS1411 on cell proliferation-toxic and radiosensitivity on cervical cancer HeLa cells, CCK-8 assay was used to determine cell proliferation-toxic effects on HeLa cells, and the effect of AS1411 on the radiosensitivity of the cells was observed by clonogenic assay. The results showed that the cell toxicity of AS1411 was very small, and all the survival rates of HeLa cells were more than 95% under the condition of different concentration of AS1411 (0 nmol/L, 50 nmol/L, 100 nmol/L, 250 nmol/L and 500 nmol/L) and the cells for 24 h, 48 h and 72 h, respectively. Clonogenic assay result shows that AS1411 combined with X ray irradiation can decrease the clonogenic survival rate of HeLa cells, and the sensitive enhancement ratio (SER) of HeLa cells are 1.06, 1.58 and 1.89, respectively with different drug concentrations of 100 nmol/L, 250 nmol/L and 500 nmol/L. This study proves AS1411 might enhance the radiosensitivity of the HeLa cells.Cited

关键词

AS1411放射敏感性HeLa细胞

Keywords

AS1411RadiosensitivityHeLa cell

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