THE CHARACTERISTICS AND MECHANISM OF APOPTOSIS IN K<sub>562</sub> CELLS INDUCED BY RADIONUCLIDES STRONTIUM-89

ZHANG Junning; HONG Chengjiao; ZHU Shoupeng

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THE CHARACTERISTICS AND MECHANISM OF APOPTOSIS IN K₅₆₂ CELLS INDUCED BY RADIONUCLIDES STRONTIUM-89

更新时间：2023-03-30

- THE CHARACTERISTICS AND MECHANISM OF APOPTOSIS IN K₅₆₂ CELLS INDUCED BY RADIONUCLIDES STRONTIUM-89
- Journal of Radiation Research and Radiation Processing Vol. 21, Issue 1, Pages: 77-80(2003)
- 作者机构：
  
  1.苏州大学附属第一医院　苏州　215006
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ZHANG Junning, HONG Chengjiao, ZHU Shoupeng. THE CHARACTERISTICS AND MECHANISM OF APOPTOSIS IN K₅₆₂ CELLS INDUCED BY RADIONUCLIDES STRONTIUM-89. [J]. Journal of Radiation Research and Radiation Processing 21(1):77-80(2003)
DOI：

ZHANG Junning, HONG Chengjiao, ZHU Shoupeng. THE CHARACTERISTICS AND MECHANISM OF APOPTOSIS IN K₅₆₂ CELLS INDUCED BY RADIONUCLIDES STRONTIUM-89. [J]. Journal of Radiation Research and Radiation Processing 21(1):77-80(2003) DOI：

摘要

采用分子病理和定量病理技术研究放射性核素,89,Sr诱导的K,562,癌细胞凋亡、剂量效应关系和重要相关基因bcl-2和bax在其中的表达。结果表明,K,562,细胞经,89,Sr作用达6h和24h,提取DNA，琼脂糖凝胶电泳图谱上出现典型的“阶梯状”区带，伴有“拖尾”,而对照组未见“阶梯状”区带；荧光双标记流式细胞仪法定量检测见,89,Sr作用6、9、12、24和48h后,K,562,细胞凋亡率、K,562,细胞坏死率均较对照组有明显的升高（,p,<,0.01）。免疫组织化学染色显示，对照组K,562,细胞中bcl-2 、bax均有表达，bcl-2表达阳性细胞率82.8%，bax表达阳性细胞率44.4%, bcl-2/bax比值1.86，,89,Sr作用24h后的K,562,细胞中, bcl-2表达阳性细胞率31.4%，bax表达阳性细胞率38.2%, bcl-2/bax比值0.82,与对照组相比，差异具有显著性（,p,<,0.05）；结果显示，放射性核素,89,Sr照射K,562,细胞后，细胞凋亡与细胞坏死并存，且,89,Sr诱导K,562,细胞的凋亡可能是通过bcl-2 表达蛋白降低，bcl-2/ bax比值下降而调控的。

Abstract

In this paper, apoptosis and expressions of bcl-2 and bax protein in K,562, cells exposed to different doses of strontium-89 were studied. Six hours after the irradiation, characteristics of apoptosis and necrosis appeared in the K,562, cells. The apoptosis and necrosis in the cells irradiated for 6, 9, 12, 24 and 48h were significantly higher than the control. The expression of bcl-2 decreased after the irradiation, whereas bax had no obvious changes. The results suggested that the apoptosis of K,562, cells induced by exposure to ,89,Sr might be regulated by reduced expression of bcl-2 and bcl-2/bax ratio.

关键词

89SrK562细胞凋亡bcl-2Bax

Keywords

Strontium-89K562 cellsApoptosisBcl-2Bax

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Department of Pathology, First Military Medical University

Institute of Radiation Medicine, Academy of Military Medical Sciences

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THE CHARACTERISTICS AND MECHANISM OF APOPTOSIS IN K562 CELLS INDUCED BY RADIONUCLIDES STRONTIUM-89

DOI：

摘要

Abstract

关键词

Keywords

references

THE CHARACTERISTICS AND MECHANISM OF APOPTOSIS IN K₅₆₂ CELLS INDUCED BY RADIONUCLIDES STRONTIUM-89