PREDICATION OF CELL RADIOSENSTITIVITY USING THE ALKALINE SINGLE CELL ELECTROPHORESIS

ZHANG Junning; HONG Chengjiao; ZHU Shoupeng

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PREDICATION OF CELL RADIOSENSTITIVITY USING THE ALKALINE SINGLE CELL ELECTROPHORESIS

更新时间：2023-03-30

- PREDICATION OF CELL RADIOSENSTITIVITY USING THE ALKALINE SINGLE CELL ELECTROPHORESIS
- Journal of Radiation Research and Radiation Processing Vol. 21, Issue 2, Pages: 106-109(2003)
- 作者机构：
  
  1.苏州大学附属第一医院　苏州　215006
  2.苏州大学核医学院　苏州　215007
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ZHANG Junning, HONG Chengjiao, ZHU Shoupeng. PREDICATION OF CELL RADIOSENSTITIVITY USING THE ALKALINE SINGLE CELL ELECTROPHORESIS. [J]. Journal of Radiation Research and Radiation Processing 21(2):106-109(2003)
DOI：

ZHANG Junning, HONG Chengjiao, ZHU Shoupeng. PREDICATION OF CELL RADIOSENSTITIVITY USING THE ALKALINE SINGLE CELL ELECTROPHORESIS. [J]. Journal of Radiation Research and Radiation Processing 21(2):106-109(2003) DOI：

摘要

应用克隆形成法和碱性单细胞凝胶电泳技术检测辐射诱导的人红白血病细胞株K,562,、人结肠腺癌细胞株LS-T-117和鼠胶质瘤细胞株C,6,的初始DNA单链断裂数及单链断裂后的修复与细胞内在放射敏感性之间的关系。结果表明，3种细胞系的放射敏感性依次为K,562,>,LS-T-117,>,C,6,；3种细胞系的DNA迁移距离都随着照射剂量的增加而增大，呈良好的剂量-效应关系。在相同剂量下，辐射诱导的DNA单链的初始断裂数目也依次为K,562,>,LS-T-117,>,C,6,；3种细胞系经10Gy X射线照射并在PBS中培养不同时间后DNA迁移距离都有较大幅度的下降，但下降幅度依次为C,6,>,LS-T-117,>,K,562,，在相同剂量下辐射诱导的DNA单链断裂后的修复能力也依次为C,6,>,LS-T-117,>, K,562,。结果显示，辐射诱导的DNA单链断裂及修复与细胞内在放射敏感性有很好的相关性，可用于人体肿瘤细胞内在放射敏感性的预测。

Abstract

The relationship between intrinsic radiosensitivity of three kinds of tumor cells, K,562, LS-T-117 and C,6, and the radiation-induced initial DNA single-strand breaks(SSBs) and SSB repair in these cells were investigateded by the alkaline comet assay.It was found that there was a close correlation between the initial SSBs and the delivered dose to cells: the larger the dose, the severer the SSB.For the same single dose, the different initial SSBs and intrinsic radiosensitivity of the cells measured by means of the clonogenic assay ranked as in a descending order: K,562,>, LS-T-117,>,C,6, while the capacity of SSB repair did as C,6,>,LS-T-117,>,K,562,. The results suggested that both radiation-induced initial SSBs and SSB repair correlated with the intrinsic radiosensitivity of cells to a certain extent, which could be used as potential predictors for the intrinsic radiosensitivity of tumor cells.

关键词

肿瘤细胞放射敏感性碱性单细胞凝胶电泳DNA单链断裂DNA单链断裂修复

Keywords

Radiosensitivity for tumor cellsAlkaline comet assayDNA single-strand breaksDNA single-strand breaks repair

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Nuclear Medical College, Suzhou University

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