THE CHEMILUMINESCENCE STUDY OF ONOO<sup>-</sup> GENERATED FROM SIN-1 AND ITS SCAVENGE BY ANTIOXIDANTS

LIANG Xiaofeng; HU Tianxi; ZHAO Yaping; YU Wenli

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THE CHEMILUMINESCENCE STUDY OF ONOO^- GENERATED FROM SIN-1 AND ITS SCAVENGE BY ANTIOXIDANTS

更新时间：2023-03-30

- THE CHEMILUMINESCENCE STUDY OF ONOO^- GENERATED FROM SIN-1 AND ITS SCAVENGE BY ANTIOXIDANTS
- Journal of Radiation Research and Radiation Processing Vol. 21, Issue 3, Pages: 206-209(2003)
- 作者机构：
  
  1.上海交大昂立公司生物医药研究所　上海　200030
  2.上海交通大学化学化工学院　上海　200240
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LIANG Xiaofeng, HU Tianxi, ZHAO Yaping, et al. THE CHEMILUMINESCENCE STUDY OF ONOO^- GENERATED FROM SIN-1 AND ITS SCAVENGE BY ANTIOXIDANTS. [J]. Journal of Radiation Research and Radiation Processing 21(3):206-209(2003)
DOI：

LIANG Xiaofeng, HU Tianxi, ZHAO Yaping, et al. THE CHEMILUMINESCENCE STUDY OF ONOO^- GENERATED FROM SIN-1 AND ITS SCAVENGE BY ANTIOXIDANTS. [J]. Journal of Radiation Research and Radiation Processing 21(3):206-209(2003) DOI：

摘要

采用3−morpholino−sydnonimine（SIN−1）作为过氧亚硝基（ONOO,-,）的发生器，以鲁米诺作为光的扩增剂，建立一个检测ONOO,-,及筛选清除剂的发光体系。在探索pH、温度、SIN−1浓度对ONOO,-,发光强度影响的基础上，确定了ONOO,-,―鲁米诺化学发光体系，测定了水溶性维生素E(Trolox)、茶多酚、槲皮素对ONOO,-,的清除作用。建成SIN−1为0.30mg/mL、鲁米诺为1×10,−4, mol•L,−1,、pH7.4、温度为30℃的发光体系，测得3种清除剂皆有清除ONOO,-,的作用，清除效果槲皮素,>,茶多酚,>,Trolox。SIN−1―鲁米诺化学发光体系测定ONOO,-,的产生和清除是一个快速、简便、灵敏且较稳定的新技术。

Abstract

Using 3-morpholino-sydnonimine (SIN-1) as peroxynitriate anion(ONOO,-,) generator and luminol as enhancer, a chemiluminescent (CL) system for screening ONOO,-, was established. Effects of pH, temperature and SIN-1 concentration on CL intensity were studied and the scavenging effects of trolox, tea polyphenol and quercetin on ONOO,-, were determined. Typical parameters of the CL system for determination ONOO,-, are SIN-1=0.30 mg/mL, luminal=1×10,-4,mol/L, pH7.4, and T=30℃. ONOO,-, scavenging abilities of quercetin, tea polyphenol and trolox were investigated with the CL system, with quercetin being the highest in the ability, whereas trolox being the lowest. The CL technique for determination of the formation and screening of ONOO,-, is quick, convenient, sensitive and steady.

关键词

SIN−1ONOO-抗氧化剂

Keywords

SIN-1ONOO-Antioxidants

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A novel chemiluminescence system for producing and measuring peroxynitrite anions

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School of Life Science, East China Normal University

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THE CHEMILUMINESCENCE STUDY OF ONOO- GENERATED FROM SIN-1 AND ITS SCAVENGE BY ANTIOXIDANTS

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THE CHEMILUMINESCENCE STUDY OF ONOO^- GENERATED FROM SIN-1 AND ITS SCAVENGE BY ANTIOXIDANTS