EXPERIMENTAL STUDY ON ANTI-TUMOR EFFECT OF pEgr-IFNγ GENE-RADIOTHERAPY IN TUMOR-BEARING MICE

YANG Wei; LI Xiuyi; LI Xiujuan; TIAN Mei; GONG Shouliang

您当前的位置：

首页 >

文章列表页 >

EXPERIMENTAL STUDY ON ANTI-TUMOR EFFECT OF pEgr-IFNγ GENE-RADIOTHERAPY IN TUMOR-BEARING MICE

更新时间：2023-03-30

- EXPERIMENTAL STUDY ON ANTI-TUMOR EFFECT OF pEgr-IFNγ GENE-RADIOTHERAPY IN TUMOR-BEARING MICE
- Journal of Radiation Research and Radiation Processing Vol. 21, Issue 3, Pages: 193-197(2003)
- 作者机构：
  
  1.吉林大学公共卫生学院卫生部放射生物学重点实验室　长春　130021
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
YANG Wei, LI Xiuyi, LI Xiujuan, et al. EXPERIMENTAL STUDY ON ANTI-TUMOR EFFECT OF pEgr-IFNγ GENE-RADIOTHERAPY IN TUMOR-BEARING MICE. [J]. Journal of Radiation Research and Radiation Processing 21(3):193-197(2003)
DOI：

YANG Wei, LI Xiuyi, LI Xiujuan, et al. EXPERIMENTAL STUDY ON ANTI-TUMOR EFFECT OF pEgr-IFNγ GENE-RADIOTHERAPY IN TUMOR-BEARING MICE. [J]. Journal of Radiation Research and Radiation Processing 21(3):193-197(2003) DOI：

摘要

探讨pEgr−IFNγ基因−放射治疗小鼠体内抗肿瘤作用的适宜照射剂量和质粒注入量。观察不同剂量X射线照射及瘤内不同量pEgr−IFNγ 重组质粒注入，基因−放射治疗在接种B16 黑色素瘤小鼠体内抑瘤效应的差异，并用酶联免疫吸附法（ELISA）法检测各组小鼠外周血IFNγ 含量。结果表明，质粒注入后接受20Gy X射线照射组照后3—27d，肿瘤生长速率明显低于2Gy 和10Gy 照射组（,p,<,0.001），且小鼠平均生存时间明显延长（,p,<,0.001）。质粒注入量为20μg 或30μg 的联合治疗组照后3—27d，肿瘤生长速率明显低于注入量为10μg的联合治疗组（,p,<,0.05—0.001），且平均生存时间明显延长（,p,<,0.01）。基因−放射联合治疗组受照射后第1天和第3天外周血IFNγ 含量明显高于单纯基因治疗组均为（,p,<,0.05）和对照组，分别为（,p,<,0.01，,p,<,0.05）。结果显示，pEgr−IFNγ 基因−放射治疗小鼠体内抗肿瘤作用的适宜照射剂量为20Gy，质粒注入量为20μg；联合治疗组可能通过诱导瘤内IFNγ 基因表达增强，使血液中的IFNγ 浓度升高，进而增强机体免疫功能和抗肿瘤能力。

Abstract

To investigate the optimal doses of X-ray irradiation and plasmid injection in the anti-tumor effect of the pEgr-IFN γ gene–radiotherapy in vivo, we observed the anti-tumor effect of the pEgr-IFN γ gene–radiotherapy with different doses of X-ray irradiation and plasmid injection in mice with B16 melanoma and detected the concentration of IFN γ in peripheral blood by ELISA. The results showed that the tumor growth rate of the mice irradiated locally with 20Gy X-rays after the plasmid injection was significantly lower (,p,<,0.001) than that of the mice irradiated locally with 2Gy or 10Gy X-ray from the third day after irradiation and the mean survival period was longer (,p,<,0.001). The results also showed that the tumor growth rate of the mice injected locally with 20μg or 30μg plasmid was significantly lower (,p,<,0.05—0.001) than that of the mice injected locally with 10μg plasmid and the mean survival period was longer (,p,<,0.01). It was found that the concentration of IFN γ in peripheral blood of the mice treated with gene-radiotherapy was higher than that of the mice of plasmid-treated group (,p,<,0.05,p,<,0.05) or control group (,p,<,0.01,p,<,0.05) on the first day and the third day after irradiation. These results suggested that the optimal dose of X-ray irradiation of the pEgr-IFN γ gene–radiotherapy in vivo was 20Gy and the optimal dose of plasmid injected into tumors was 20μg. By inducing the higher expression of IFN γ gene in the tumor, the pEgr-IFN γ gene–radiotherapy could increase the concentration of IFN γ in peripheral blood, so as to enhance the body’s immunologic function and anti-tumor ability.

关键词

pEgr−IFNγ重组质粒基因−放射治疗B16黑色素瘤X射线

Keywords

pEgr-IFN γ reconstruction plasmidGene –radiotherapyB16 melanomaX-ray

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Study on the dose uniformity of multi-channel parallel diode

Numerical simulation of the radiation field parameters of different composite anode targets

anti-tumor effect of silencing Hif-1α and Survivin genes combined with X-ray irradiation on hypoxic tumor cells in vitro

Expression and localization of Smad4 protein in mouse testis after whole-body X-ray irradiation

COMPARISON OF X-AND γ-RADIATION DAMAGE ON OPERATIONAL AMPLIFIERS

Related Author

No data

Related Institution

National Key Laboratory of Intense Pulsed Radiation Simulation and Effect, Northwest Institute of Nuclear Technology, Xi´an

Department of Radiobiology, School of Radiological Medicine and Public Health, Soochow University

Department of orthopedics, PLA 403th Hospital

Department of Histology and Embryology, Fourth Military University

Department ofRadiotherapy, Tangdu Hospital, the Fourth Military University

⁰