Construction of Egr−IFNγ and it’s expression in B16 cells induced by ionizing irradiation

Wu Congmei; Li Xiuyi; Tian mei; Liu Shuzheng

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Construction of Egr−IFNγ and it’s expression in B16 cells induced by ionizing irradiation

更新时间：2023-03-30

- Construction of Egr−IFNγ and it’s expression in B16 cells induced by ionizing irradiation
- Journal of Radiation Research and Radiation Processing Vol. 21, Issue 4, Pages: 268-271(2003)
- 作者机构：
  
  1.汕头大学医学院　汕头　515031
  2.吉林大学放射生物重点实验室　长春　130021
- 作者简介：
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Wu Congmei, Li Xiuyi, Tian mei, et al. Construction of Egr−IFNγ and it’s expression in B16 cells induced by ionizing irradiation. [J]. Journal of Radiation Research and Radiation Processing 21(4):268-271(2003)
DOI：

Wu Congmei, Li Xiuyi, Tian mei, et al. Construction of Egr−IFNγ and it’s expression in B16 cells induced by ionizing irradiation. [J]. Journal of Radiation Research and Radiation Processing 21(4):268-271(2003) DOI：

摘要

小鼠IFNγ cDNA基因连接到Egr-1启动子的下游构建成Egr−IFNγ重组质粒，利用质脂体介导转染B16细胞，用ELISA方法检测X射线照射后IFNγ表达的剂量效应和时程。结果表明，实验组的表达水平明显高于对照组，其中20Gy和2Gy照射组高于其它实验组（,p<,0.01）。2Gy照射后6h IFNγ表达量最高（,p<,0.01）。转染后的B16细胞每隔24h接受2Gy X射线照射，结果显示第一次照射后表达量最高（,p<,0.01），然后逐渐降低。结果提示电离辐射可激活Egr−IFNγ重组质粒，并调节IFNγ基因表达，这项研究可能对肿瘤治疗的研究有一定的意义。

Abstract

Mouse IFNγ cDNA was ligated to downstream of Egr−1 promoter to construct Egr−IFNγ plasmid. The recombined plasmids were transfected into B16 cells with lipsome, then the expression of IFNγ after different doses of X−ray irradiation and the time course of expression were detected by ELISA. The results showed that the expression levels in experimental groups were higher than that in the control group obviously (,p<,0.01); 6h after 2Gy X−ray irradiation, the expression of IFNγ was highest (,p<,0.01); The B16 cells transfected were given 2Gy X−ray irradiation every 24h, the expression level was highest after first irradiation (,p<,0.01)and then decrease gradually. The results indicated that ionizing irradiation can activate the Egr−IFNγ recombined plasmid and regulate the expression of IFNγ gene in B16 cells. The observation may be of potential significance in tumor therapy.

关键词

Egr−1启动子X射线照射IFNγ表达ELISA

Keywords

Egr−1 promoterX−ray irradiationIFNγ expressionELISA

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