p16 gene transcription and protein expression in EL-4 cells irradiated with X-rays in vitro

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p16 gene transcription and protein expression in EL-4 cells irradiated with X-rays in vitro

更新时间：2023-03-30

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- p16 gene transcription and protein expression in EL-4 cells irradiated with X-rays in vitro
- Journal of Radiation Research and Radiation Processing Vol. 22, Issue 2, Pages: 123-125(2004)
- 作者机构：
  
  1.汕头大学医学院生物教研室　汕头　515031
  2.吉林大学卫生部放射生物重点实验室　长春　130021
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WANG Xiaomei, JU Guizhi, MEI Shujiang, et al. p16 gene transcription and protein expression in EL-4 cells irradiated with X-rays in vitro. [J]. Journal of Radiation Research and Radiation Processing 22(2):123-125(2004)
DOI：

WANG Xiaomei, JU Guizhi, MEI Shujiang, et al. p16 gene transcription and protein expression in EL-4 cells irradiated with X-rays in vitro. [J]. Journal of Radiation Research and Radiation Processing 22(2):123-125(2004) DOI：

摘要

采用RT−PCR（逆转录聚合酶链反应）半定量方法检测p16基因转录水平的变化，采用免疫细胞化学方法检测蛋白表达，观察X射线照射对离体培养的EL−4细胞p16基因转录及蛋白表达的影响，证实p16基因转录表达在辐射诱导EL−4细胞G,1,期阻滞中的重要作用。结果表明，2.0Gy X射线照射后2—48h，EL−4 细胞p16mRNA 水平增高，照射后72h恢复正常水平。P16蛋白表达于照射后2h开始增高，照射后12h P16蛋白表达非常显著高于对照组（,p,<,0.01）。剂量−效应实验表明，0.5—6.0Gy X射线照射后12h，EL−4细胞p16mRNA水平明显提高。2.0、4.0、6.0Gy组P16蛋白表达均非常显著增加（分别,p,<,0.01）。研究证实，电离辐射能够诱导EL−4细胞p16基因转录及蛋白表达增高，其增幅呈现明显的剂量依赖性。提示辐射诱导p16基因转录表达增加在辐射诱导EL−4细胞G,1,期阻滞中发挥重要作用。

Abstract

To investigate the effect of ionizing radiation on p16 gene transcription and protein expression in EL-4 cells in vitro, RT-PCR semi-quantity method was applied to determine the p16mRNA level and immunocytochemistry (ICC) was employed to measure the p16 protein expression. The time-course experiments showed that the p16 mRNA level increased at 2—48h in EL-4 cells, and the p16 protein expression increased significantly in EL-4 cells (,p,<,0.01) at 12h after 2.0Gy X-irradiation. The dose-effect experiments showed that the p16mRNA level increased with the dose, and the p16 protein expression increased significantly (,p,<,0.01) with the dose in 2.0 - 6.0Gy range. The results suggested that the increase of p16 gene transcription and protein expression in EL-4 cells might play role in G1 arrest induced by ionizing radiation in vitro.

关键词

X射线G1期阻滞p16蛋白p16mRMA

Keywords

X-raysG1 arrestp16 proteinp16 mRNA

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