The apoptosis of CHO cells induced by X-rays

LU Zhaohong; ZHAO Jingyong; ZHU Mingqing; SHI Xijin; WANG Chunlei

您当前的位置：

首页 >

文章列表页 >

The apoptosis of CHO cells induced by X-rays

更新时间：2023-03-30

- The apoptosis of CHO cells induced by X-rays
- Journal of Radiation Research and Radiation Processing Vol. 22, Issue 5, Pages: 307-310(2004)
- 作者机构：
  
  1.苏州大学放射医学与公共卫生学院　苏州　215007
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
LU Zhaohong, ZHAO Jingyong, ZHU Mingqing, et al. The apoptosis of CHO cells induced by X-rays. [J]. Journal of Radiation Research and Radiation Processing 22(5):307-310(2004)
DOI：

LU Zhaohong, ZHAO Jingyong, ZHU Mingqing, et al. The apoptosis of CHO cells induced by X-rays. [J]. Journal of Radiation Research and Radiation Processing 22(5):307-310(2004) DOI：

摘要

为研究X射线致中国仓鼠卵巢（CHO）细胞凋亡，探讨电离辐射的生殖毒性机理，用2—20Gy X射线照射CHO细胞株，通过荧光显微镜、Annexin V/PI双染色法结合流式细胞仪，检测和观察细胞凋亡及其形态学改变。结果表明，X射线照射后24、48、72h均出现细胞凋亡，并随照射剂量增加凋亡细胞数亦增加。但照射后48h和72h细胞凋亡更为明显（,p,<,0.05）。凋亡的CHO细胞在荧光显微镜下，观察到细胞核内染色质的不规则聚集、核固缩和周边化，有的呈月牙形，甚至出现凋亡小体等一系列凋亡细胞形态特征。结果显示：电离辐射引起生殖细胞严重的DNA损伤，因无法修复继而导致细胞凋亡。

Abstract

The work is to study the mechanism of toxic effects on reproductive system and apoptosis of Chinese hamster ovary (CHO) cells induced by X-rays. CHO cell was exposed to X-rays 2 to 20Gy, Apoptosis and morphological changes of the cells were observed by fluorescent microscopy and flow cytometry analyzer with double staining with Annexin V/ PI. The apoptosis could be observed at 24, 48 and 72h after the exposure, but it was more obvious 48 and 72 h after the exposure. Rate of the apoptosis increased along with radiation dose were elevated. Some morphologyical changes, such as irregular agglomerate of chromatins, pycnosis and periphery distribution of nuclei, crescent-moon-like cell, small apoptosis body, were observed. Radiation results DNA damage in the CHO cells, and the demage cannot be repaired, hence the induced cell apoptosis.

关键词

电离辐射中国仓鼠卵巢细胞细胞凋亡

Keywords

Ionizing radiationCHO cellsApoptosis

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Quercetin promotes apoptosis in ionizing radiation-induced senescent cells

INFLUENCES OF IONIZING RADIATION ON APOPTOSIS AND CELL CYCLE OF HelaS₃ CELL LINES

EFFECT OF IONIZING RADIATION ON APOPTOSIS IN MOUSE PEYER'S PATCHES

THE EFFECTS OF p53 AND Bcl — 2/Bax ON APOPTOSIS OF THYMOCYTES INDUCED BY IONIZING RADIATION

Preparation of hydrogel-based photocatalytic composite materials by radiation technology

Related Author

No data

Related Institution

School of Pharmacy, Lanzhou University

Key Laboratory of Space Radiobiology of Gansu Province, Institute of Modern Physics, Chinese Academy of Science

University of Chinese Academy of Sciences

MH Radiobiology Research Unit, Normen Bathune University of Medical Sciences

Department of Histology and Embryology Norman Bethune University of Medical Sciences

⁰