3D structural changes and DNA damages to γ ray irradiated C6, SGH-44 and NCI-H446 tumor cells

LIU Fenju; CHEN Jian; NING Ping; HUANG Hui; LI Bingyan

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3D structural changes and DNA damages to γ ray irradiated C6, SGH-44 and NCI-H446 tumor cells

Radiation Bio-medicine | 更新时间：2023-03-30

- 3D structural changes and DNA damages to γ ray irradiated C6, SGH-44 and NCI-H446 tumor cells
- Journal of Radiation Research and Radiation Processing Vol. 23, Issue 2, Pages: 86-87(2005)
- 作者机构：
  
  1.苏州大学放射医学与公共卫生学院　苏州　215007
  2.放射医学与防护省重点实验室　苏州　215006
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LIU Fenju, CHEN Jian, NING Ping, et al. 3D structural changes and DNA damages to γ ray irradiated C6, SGH-44 and NCI-H446 tumor cells. [J]. Journal of Radiation Research and Radiation Processing 23(2):86-87(2005)
DOI：

LIU Fenju, CHEN Jian, NING Ping, et al. 3D structural changes and DNA damages to γ ray irradiated C6, SGH-44 and NCI-H446 tumor cells. [J]. Journal of Radiation Research and Radiation Processing 23(2):86-87(2005) DOI：

摘要

脑胶质瘤是常见恶性肿瘤，我国脑胶质瘤发病率占脑肿瘤40%以上，占全部肿瘤的1%—3%，由于血-脑屏障作用，神经胶质瘤对射线并不敏感，放疗的治疗比值较小，化疗也不理想。寻找神经胶质瘤细胞的耐辐射基因，探讨其耐辐射机制，可望为脑胶质瘤放疗增敏提供指导，提高患者术后的生存率。本研究用小鼠脑胶质瘤（C6）、人脑胶质瘤（SHG-44）和小细胞肺癌（NCI-H446）等细胞株、用单细胞凝胶电泳技术和激光共聚扫描显微镜（Laser scanning confocal microscopy，LSCM），观察γ射线照射细胞的三维图象，研究γ射线所致DNA链断裂及“DNA/RNA”补英文全称和中文说明。脱氧核糖核酸与核糖核酸的比值（Deoxyribonucleic acid / ribonucleic acid，DNA/RNA）含量比值的变化。

Abstract

Because of its low sensitivity to radiations, therapeutic ratio of neuroglioma is relatively small. And chemotherapy is not ideal to the disease, either. Therefore, studies are needed towards finding radio-resistance gene. Human cerebral glioma SHG-44, mouse cerebral glioma C6, and small cell lung cancer NCI-H446 (for comparison), were irradiated by ,60,Co γ-rays to 1, 3 and 8Gy, and changes of DNA/RNA ratios in the cells were studied with LSCM (laser scanning confocal microscopy). It was found that the fluorescence dye of DNA in three kinds of cells in the control group is significantly higher than the RNA fluorescence dye (,p,<,0.01). Degree of the damages to the irradiated NCI-H446 cells increased gradually with the dose, and damages to the cerebral glioma cells was less severe at the same radiation dose as the NCI-H446 cells.

关键词

共聚焦显微镜细胞株（CNI-H446、C6、SHG-44）脱氧核糖核酸核糖核酸60Co γ辐照

Keywords

Confocal microscopeCell strains (CNI- H446C6SHG-44)Deoxyribonucleic acid (DNA)Ribonucleic acid (RNA)60 Co γ-irradiation

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