15 keV N<sup>+</sup> implantation to induce mutation of protopectinase-producing<italic style="font-style: italic"> Aspergillus sp.</italic>

ZHANG Yiqing; LU Zhaoxin; ZOU Xiaokui

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15 keV N⁺ implantation to induce mutation of protopectinase-producing Aspergillus sp.

更新时间：2023-03-30

- 15 keV N⁺ implantation to induce mutation of protopectinase-producing Aspergillus sp.
- Journal of Radiation Research and Radiation Processing Vol. 23, Issue 3, Pages: 140-144(2005)
- 作者机构：
  
  1.南京农业大学食品科技学院　南京　210095
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ZHANG Yiqing, LU Zhaoxin, ZOU Xiaokui. 15 keV N⁺ implantation to induce mutation of protopectinase-producing Aspergillus sp.. [J]. Journal of Radiation Research and Radiation Processing 23(3):140-144(2005)
DOI：

ZHANG Yiqing, LU Zhaoxin, ZOU Xiaokui. 15 keV N⁺ implantation to induce mutation of protopectinase-producing Aspergillus sp.. [J]. Journal of Radiation Research and Radiation Processing 23(3):140-144(2005) DOI：

摘要

原果胶酶是一种具有广泛应用前景的工业酶类，为获得高活性原果胶酶的菌株，用能量为15keV、注量为20×2.6×10,13,—180×2.6×10,13,cm,-2,的N,+,注入原果胶酶产生菌黑曲霉,Aspergillus sp., XZ-131的成熟孢子，其存活率曲线为典型的“马鞍型”剂量-效应曲线。经筛选确定高产诱变菌株Z-25，经传代培养，高产性状稳定遗传。发酵过程中比原始菌株提前24h进入生长旺盛期，随后在稳定期期间大量产酶，发酵至72h时酶活性达到最高，比原始菌株提高了179%，每克干细胞性比原始菌株提高了84%。

Abstract

Protopectinase will be of potential use in industry. The strain of protopectinase-producing, Aspergillus sp., XZ-131 was mutagenized by 15keV N,+, ion implantation, with a dose range of 0.52×10,15, ion/cm,2, to 4.68×10,15, ion/cm,2,. From the ion implanted ,Aspergillus sp., XZ-131, the Strain Z-25, which yields high activity protopectinase, was screened out. The protopectinase activity increased by 179% and the protopectinase (U/g dry cell) increased by 84%. In addition, the exponential phase of, Aspergillus sp., Z-25 was 24 hours shorter than the Strain XZ-131. The ,Aspergillus sp., Z-25 yielded high protopectinase activity during stationary phase, the highest protopectinase activity was achieved after 72 h fermentation.

关键词

离子注入原果胶酶诱变筛选育种发酵

Keywords

Ion implantationProtopectinaseMutationScreeningBreedingFermentation process

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15 keV N+ implantation to induce mutation of protopectinase-producing Aspergillus sp.

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摘要

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15 keV N⁺ implantation to induce mutation of protopectinase-producing Aspergillus sp.