Breeding of citric acid-producing bacteria by 15 keV N<sup>+</sup> ion implantation

YU Long; PAN Tao; ZHOU Jian

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Breeding of citric acid-producing bacteria by 15 keV N⁺ ion implantation

更新时间：2023-03-30

- Breeding of citric acid-producing bacteria by 15 keV N⁺ ion implantation
- Journal of Radiation Research and Radiation Processing Vol. 23, Issue 4, Pages: 233-236(2005)
- 作者机构：
  
  1.南京工业大学制药与生命科学学院　南京　210009
  2.江苏广播电视大学　南京　210036
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YU Long, PAN Tao, ZHOU Jian. Breeding of citric acid-producing bacteria by 15 keV N⁺ ion implantation. [J]. Journal of Radiation Research and Radiation Processing 23(4):233-236(2005)
DOI：

YU Long, PAN Tao, ZHOU Jian. Breeding of citric acid-producing bacteria by 15 keV N⁺ ion implantation. [J]. Journal of Radiation Research and Radiation Processing 23(4):233-236(2005) DOI：

摘要

用15keV氮离子注入对柠檬酸生产菌进行诱变选育，对该低能离子与微生物间的作用规律进行了初步探索，并获得三株高产突变菌株。其中，以木薯为底物的突变菌株的柠檬酸产率提高14.2%，以玉米粉为底物的两株突变菌株的柠檬酸产率分别提高15.9%和17.0%，经多次传代实验表明，这三种突变菌株均有较好遗传稳定性。

Abstract

Aspergillus niger, strains CK1−16, the bacteria commonly used in industrial production of citric acid, were implanted with 3.10×10,14, to 2.58×10,15, ions/cm,2, of 15 keV N,+, ions . Suevival rate of the bacteria at different doses was investigated, and mutagenic effects of the microbe were studied. From the ion-implanted specimens, we were able to obtain three mutant strains that produce increased yield of citric acid. The 71 hours yield of Mutant Strain 4#−8−1incubated in cassava and corn flour media is 14% higher than the CK1-16 strain, while Mutant Strains 4#−8−7 and 4#−8−7, incubated in corn flour media are 15.9% and 17.0% higher than the CK1-16 strain, respectively. The results also show that the mutant strains have high genetic stability.

关键词

离子注入柠檬酸诱变选育

Keywords

Ion implantationCitric acidMutationBreed

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Breeding of citric acid-producing bacteria by 15 keV N+ ion implantation

DOI：

摘要

Abstract

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Breeding of citric acid-producing bacteria by 15 keV N⁺ ion implantation